Plant Crispr Database

Plant Information

Name: CRT0137-19-1-14-3

Seed stock: 5039
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0137-19-1-14

Transformation: CRT0137
transformation CRT0137 to M82 sp+

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0137-19-1-14-3 (T3 )	CRT0137	CTG0137-48	CGR0137-48	gene : Solyc10g085120 (LRRXII-RLK)	azygous Is tDNA present: No Mutation sequence: CCAAGCAACATACTCCTCGATGAAGATATGTCTGCCCATGTTGGTGACTTTGGATTAGCAACATTTCTCCTCGACACATC AAGTAATTCTTGGAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAACAGGTACAACGATGTCAT TAGTTTACATTTTAATATTCAGTCATATATCATGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGC CAACCATCCACACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGTTGTTCATCTGTAAAAGACCAACTGATGC AATTTTCAACGAGAGTCTAAACATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGTAGACCCCT TATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATCAAGCAAGAAGAGTGGAAGAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2575 GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTG 2624 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTG 50 Wildtype 2625 GAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAA 2674 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 GAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAA 100 Wildtype 2675 CAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCA 2724 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 CAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCA 150 Wildtype 2725 TGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCA 2774 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 TGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCA 200 gRNA GGCGGCCA Wildtype 2775 ACCATCCACACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGT 2824 \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 ACCATCCA-ACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGT 249 gRNA GGCGGCCAACCATCCACACT Wildtype 2825 TGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAAC 2874 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 250 TGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAAC 299 Wildtype 2875 ATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGT 2924 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 300 ATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGT 349 Wildtype 2925 AGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATC 2974 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 350 AGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATC 399 Wildtype 2975 AAGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG 3021 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 400 AAGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG 446

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0137-19-1-14-3 (T3 )

CRT0137

CTG0137-48

CGR0137-48

gene : Solyc10g085120 (LRRXII-RLK)

azygous

Is tDNA present: No

Mutation sequence:

CCAAGCAACATACTCCTCGATGAAGATATGTCTGCCCATGTTGGTGACTTTGGATTAGCAACATTTCTCCTCGACACATC
AAGTAATTCTTGGAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAACAGGTACAACGATGTCAT
TAGTTTACATTTTAATATTCAGTCATATATCATGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGC
CAACCATCCACACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGTTGTTCATCTGTAAAAGACCAACTGATGC
AATTTTCAACGAGAGTCTAAACATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGTAGACCCCT
TATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATCAAGCAAGAAGAGTGGAAGAAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2575 GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTG   2624
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTG     50

Wildtype        2625 GAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAA   2674
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAA    100

Wildtype        2675 CAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCA   2724
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCA    150

Wildtype        2725 TGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCA   2774
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCA    200
gRNA                                                           GGCGGCCA

Wildtype        2775 ACCATCCACACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGT   2824
                     |||||||| |||||||||||||||||||||||||||||||||||||||||
Mutation         201 ACCATCCA-ACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGT    249
gRNA                 GGCGGCCAACCATCCACACT

Wildtype        2825 TGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAAC   2874
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 TGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAAC    299

Wildtype        2875 ATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGT   2924
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 ATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGT    349

Wildtype        2925 AGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATC   2974
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 AGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATC    399

Wildtype        2975 AAGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG   3021
                     |||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 AAGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG    446