Plant Crispr Database

Plant Information

Name: CRT0137-4-2

Seed stock: 4768
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0137-4
Progeny: CRT0137-4-2-1
Progeny: CRT0137-4-2-2

Transformation: CRT0137
transformation CRT0137 to M82 sp+

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0137-4-2 (T1 )	CRT0137	CTG0137-14	CGR0137-14	gene : Solyc03g118330 (LRRXII-RLK)	-1 bp/-2112 bp
CRT0137-4-2 (T1 )	CRT0137	CTG0137-24	CGR0137-24	gene : Solyc04g014900 (LRRXII-RLK)	-1 bp/-1 bp Is tDNA present: No Mutation sequence: GTGTTGGAGGAATGGGACCTGTTAATTGATTATCTCTCAAGGATAAAAATGCAAGCTGGCTCAGATTACCGACCTCCGTT GGAATGTTGCCGCTGACTCTATTCCCAGACAAACTGAAGTTCATCATTTTTGTGGCATTTCCAACAGAAGGGGGGATTAT ACCGGTGAGGCTATTTTTCCTGAGATTTAAGACTCTGAGTTGGGGTACATACCATGGCCCTCTCCACATTTCACCCAATG AAATTACTTCAACTCTACGGTTTTGAAATAGACTTGTTGGTATACTTCCGTTGAGCTGATTGTTTTTAACATCAATCACT CGCAAGCGAGGCAGGTGACCAAGTCCTAAGGGATGCCACCGTGGAAGCTGTTATTCTGGAGATTGAGAACACTGAGGAAG GACAAATTGGCCAAAGACGGGGAAATTGTGCCTTGAAGTTGCAAATTAGGAAGATCCAAGGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1418 GTGTTGGAGGAATGGGACCTGTTAATTGATTATCTCTCAAGGATAAAAAT 1467 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 GTGTTGGAGGAATGGGACCTGTTAATTGATTATCTCTCAAGGATAAAAAT 50 Wildtype 1468 GCAAGCTGGCTCAGATTACCGACCTCCGTTGGAATGTTGCCGCTGACTCT 1517 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 GCAAGCTGGCTCAGATTACCGACCTCCGTTGGAATGTTGCCGCTGACTCT 100 Wildtype 1518 ATTCCCAGACAAACTGAAGTTCATCATTTTTGTGGCATTTCCAACAGAAG 1567 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 ATTCCCAGACAAACTGAAGTTCATCATTTTTGTGGCATTTCCAACAGAAG 150 Wildtype 1568 GGGGGATTATACCGGTGAGGCTATTTTTCCTGAGATTTAAGACTCTGAGT 1617 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 GGGGGATTATACCGGTGAGGCTATTTTTCCTGAGATTTAAGACTCTGAGT 200 Wildtype 1618 TGGGGTACATACCATGGCCCTCTCCACATTTCACCCAATGAAATTACTTC 1667 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 TGGGGTACATACCATGGCCCTCTCCACATTTCACCCAATGAAATTACTTC 250 Wildtype 1668 AACTCTACGGTTTTGAAATAGACTTGTTGGTATACTTCCGTTGAGCTGAT 1717 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 AACTCTACGGTTTTGAAATAGACTTGTTGGTATACTTCCGTTGAGCTGAT 300 Wildtype 1718 TGTTTTTAACATCAATCACTCGCAAGCGAGGCAGGTGACCAAGTCCATAA 1767 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Mutation 301 TGTTTTTAACATCAATCACTCGCAAGCGAGGCAGGTGACCAAGTCC-TAA 349 gRNA GCAGGTGACCAAGTCCATAA Wildtype 1768 GGGATGCCACCGTGGAAGCTGTTATTCTGGAGATTGAGAACACTGAGGAA 1817 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 350 GGGATGCCACCGTGGAAGCTGTTATTCTGGAGATTGAGAACACTGAGGAA 399 Wildtype 1818 GGACAAATTGGCCAAAGACGGGGAAATTGTGCCTTGAAGTTGCAAATTAG 1867 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 400 GGACAAATTGGCCAAAGACGGGGAAATTGTGCCTTGAAGTTGCAAATTAG 449 Wildtype 1868 GAAGATCCAAGGCA 1881 \|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 450 GAAGATCCAAGGCA 463

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0137-4-2 (T1 )

CRT0137

CTG0137-14

CGR0137-14

gene : Solyc03g118330 (LRRXII-RLK)

-1 bp/-2112 bp

CRT0137-4-2 (T1 )

CRT0137

CTG0137-24

CGR0137-24

gene : Solyc04g014900 (LRRXII-RLK)

-1 bp/-1 bp

Is tDNA present: No

Mutation sequence:

GTGTTGGAGGAATGGGACCTGTTAATTGATTATCTCTCAAGGATAAAAATGCAAGCTGGCTCAGATTACCGACCTCCGTT
GGAATGTTGCCGCTGACTCTATTCCCAGACAAACTGAAGTTCATCATTTTTGTGGCATTTCCAACAGAAGGGGGGATTAT
ACCGGTGAGGCTATTTTTCCTGAGATTTAAGACTCTGAGTTGGGGTACATACCATGGCCCTCTCCACATTTCACCCAATG
AAATTACTTCAACTCTACGGTTTTGAAATAGACTTGTTGGTATACTTCCGTTGAGCTGATTGTTTTTAACATCAATCACT
CGCAAGCGAGGCAGGTGACCAAGTCCTAAGGGATGCCACCGTGGAAGCTGTTATTCTGGAGATTGAGAACACTGAGGAAG
GACAAATTGGCCAAAGACGGGGAAATTGTGCCTTGAAGTTGCAAATTAGGAAGATCCAAGGCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1418 GTGTTGGAGGAATGGGACCTGTTAATTGATTATCTCTCAAGGATAAAAAT   1467
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 GTGTTGGAGGAATGGGACCTGTTAATTGATTATCTCTCAAGGATAAAAAT     50

Wildtype        1468 GCAAGCTGGCTCAGATTACCGACCTCCGTTGGAATGTTGCCGCTGACTCT   1517
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GCAAGCTGGCTCAGATTACCGACCTCCGTTGGAATGTTGCCGCTGACTCT    100

Wildtype        1518 ATTCCCAGACAAACTGAAGTTCATCATTTTTGTGGCATTTCCAACAGAAG   1567
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATTCCCAGACAAACTGAAGTTCATCATTTTTGTGGCATTTCCAACAGAAG    150

Wildtype        1568 GGGGGATTATACCGGTGAGGCTATTTTTCCTGAGATTTAAGACTCTGAGT   1617
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GGGGGATTATACCGGTGAGGCTATTTTTCCTGAGATTTAAGACTCTGAGT    200

Wildtype        1618 TGGGGTACATACCATGGCCCTCTCCACATTTCACCCAATGAAATTACTTC   1667
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TGGGGTACATACCATGGCCCTCTCCACATTTCACCCAATGAAATTACTTC    250

Wildtype        1668 AACTCTACGGTTTTGAAATAGACTTGTTGGTATACTTCCGTTGAGCTGAT   1717
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AACTCTACGGTTTTGAAATAGACTTGTTGGTATACTTCCGTTGAGCTGAT    300

Wildtype        1718 TGTTTTTAACATCAATCACTCGCAAGCGAGGCAGGTGACCAAGTCCATAA   1767
                     |||||||||||||||||||||||||||||||||||||||||||||| |||
Mutation         301 TGTTTTTAACATCAATCACTCGCAAGCGAGGCAGGTGACCAAGTCC-TAA    349
gRNA                                               GCAGGTGACCAAGTCCATAA

Wildtype        1768 GGGATGCCACCGTGGAAGCTGTTATTCTGGAGATTGAGAACACTGAGGAA   1817
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 GGGATGCCACCGTGGAAGCTGTTATTCTGGAGATTGAGAACACTGAGGAA    399

Wildtype        1818 GGACAAATTGGCCAAAGACGGGGAAATTGTGCCTTGAAGTTGCAAATTAG   1867
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 GGACAAATTGGCCAAAGACGGGGAAATTGTGCCTTGAAGTTGCAAATTAG    449

Wildtype        1868 GAAGATCCAAGGCA   1881
                     ||||||||||||||
Mutation         450 GAAGATCCAAGGCA    463