Plant Crispr Database

Plant Information

Name: CRT0137-8-3

Seed stock: 4778
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0137-8
Progeny: CRT0137-8-3-4
Progeny: CRT0137-8-3-3
Progeny: CRT0137-8-3-1

Transformation: CRT0137
transformation CRT0137 to M82 sp+

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0137-8-3 (T1 )	CRT0137	CTG0137-10	CGR0137-10	gene : Solyc03g006100 (LRRXII-RLK)	-5 bp/-5 bp Is tDNA present: No Mutation sequence: ACTGCCATTGCAGTTAAAGTGTTCAATCTGCAACTGGAAGCGGCATTCAAGAGTTTTGATACAGAATGTGAAGTGTTGTG CAGCCTTCGCCACAGGAATCTCGTGAAAGTCATCACTAGTTGCTCCAACCTGGATTTTAAGGCTTTAGTGCTCGAGTATA TGCCTAATGGAAGTCTTGACAAGTATTTGTATTCGCACAACTACTTCCTAGATATCAGCCAGAGACTAAGCATTATGATA GATGTGGCATGTGCTTTGGAATAACCACGGATGCTCCTCGCCCGTGATTCACTGTGATCTAAAGCCCAGTAACATCTTGT TGGATGAGGATATGGTTGCTCACCTAGGTGACTTTGGTATTTCAAAACTGCTTGGTGAAGATGAGAGTGATTTATACACA AAAACCTTAGCAACATTTGGTTATATTGCACCGGGTACGTCTCTTTGTTTTTGCTAATTTGAGCATTGATTTTTCATCTT TTT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2720 ACTGCCATTGCAGTTAAAGTGTTCAATCTGCAACTGGAAGCGGCATTCAA 2769 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 ACTGCCATTGCAGTTAAAGTGTTCAATCTGCAACTGGAAGCGGCATTCAA 50 Wildtype 2770 GAGTTTTGATACAGAATGTGAAGTGTTGTGCAGCCTTCGCCACAGGAATC 2819 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 GAGTTTTGATACAGAATGTGAAGTGTTGTGCAGCCTTCGCCACAGGAATC 100 Wildtype 2820 TCGTGAAAGTCATCACTAGTTGCTCCAACCTGGATTTTAAGGCTTTAGTG 2869 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TCGTGAAAGTCATCACTAGTTGCTCCAACCTGGATTTTAAGGCTTTAGTG 150 Wildtype 2870 CTCGAGTATATGCCTAATGGAAGTCTTGACAAGTATTTGTATTCGCACAA 2919 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 CTCGAGTATATGCCTAATGGAAGTCTTGACAAGTATTTGTATTCGCACAA 200 Wildtype 2920 CTACTTCCTAGATATCAGCCAGAGACTAAGCATTATGATAGATGTGGCAT 2969 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 CTACTTCCTAGATATCAGCCAGAGACTAAGCATTATGATAGATGTGGCAT 250 Wildtype 2970 GTGCTTTGGAATATCTTCACCACGGATGCTCCTCGCCCGTGATTCACTGT 3019 \|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 GTGCTTTGGAATA-----ACCACGGATGCTCCTCGCCCGTGATTCACTGT 295 gRNA GCTTTGGAATATCTTCACCA Wildtype 3020 GATCTAAAGCCCAGTAACATCTTGTTGGATGAGGATATGGTTGCTCACCT 3069 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 296 GATCTAAAGCCCAGTAACATCTTGTTGGATGAGGATATGGTTGCTCACCT 345 Wildtype 3070 AGGTGACTTTGGTATTTCAAAACTGCTTGGTGAAGATGAGAGTGATTTAT 3119 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 346 AGGTGACTTTGGTATTTCAAAACTGCTTGGTGAAGATGAGAGTGATTTAT 395 Wildtype 3120 ACACAAAAACCTTAGCAACATTTGGTTATATTGCACCGGGTACGTCTCTT 3169 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 396 ACACAAAAACCTTAGCAACATTTGGTTATATTGCACCGGGTACGTCTCTT 445 Wildtype 3170 TGTTTTTGCTAATTTGAGCATTGATTTTTCATCTTTTT 3207 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 446 TGTTTTTGCTAATTTGAGCATTGATTTTTCATCTTTTT 483	Morphology : Serrated cotyledons

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0137-8-3 (T1 )

CRT0137

CTG0137-10

CGR0137-10

gene : Solyc03g006100 (LRRXII-RLK)

-5 bp/-5 bp

Is tDNA present: No

Mutation sequence:

ACTGCCATTGCAGTTAAAGTGTTCAATCTGCAACTGGAAGCGGCATTCAAGAGTTTTGATACAGAATGTGAAGTGTTGTG
CAGCCTTCGCCACAGGAATCTCGTGAAAGTCATCACTAGTTGCTCCAACCTGGATTTTAAGGCTTTAGTGCTCGAGTATA
TGCCTAATGGAAGTCTTGACAAGTATTTGTATTCGCACAACTACTTCCTAGATATCAGCCAGAGACTAAGCATTATGATA
GATGTGGCATGTGCTTTGGAATAACCACGGATGCTCCTCGCCCGTGATTCACTGTGATCTAAAGCCCAGTAACATCTTGT
TGGATGAGGATATGGTTGCTCACCTAGGTGACTTTGGTATTTCAAAACTGCTTGGTGAAGATGAGAGTGATTTATACACA
AAAACCTTAGCAACATTTGGTTATATTGCACCGGGTACGTCTCTTTGTTTTTGCTAATTTGAGCATTGATTTTTCATCTT
TTT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2720 ACTGCCATTGCAGTTAAAGTGTTCAATCTGCAACTGGAAGCGGCATTCAA   2769
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ACTGCCATTGCAGTTAAAGTGTTCAATCTGCAACTGGAAGCGGCATTCAA     50

Wildtype        2770 GAGTTTTGATACAGAATGTGAAGTGTTGTGCAGCCTTCGCCACAGGAATC   2819
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GAGTTTTGATACAGAATGTGAAGTGTTGTGCAGCCTTCGCCACAGGAATC    100

Wildtype        2820 TCGTGAAAGTCATCACTAGTTGCTCCAACCTGGATTTTAAGGCTTTAGTG   2869
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCGTGAAAGTCATCACTAGTTGCTCCAACCTGGATTTTAAGGCTTTAGTG    150

Wildtype        2870 CTCGAGTATATGCCTAATGGAAGTCTTGACAAGTATTTGTATTCGCACAA   2919
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCGAGTATATGCCTAATGGAAGTCTTGACAAGTATTTGTATTCGCACAA    200

Wildtype        2920 CTACTTCCTAGATATCAGCCAGAGACTAAGCATTATGATAGATGTGGCAT   2969
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CTACTTCCTAGATATCAGCCAGAGACTAAGCATTATGATAGATGTGGCAT    250

Wildtype        2970 GTGCTTTGGAATATCTTCACCACGGATGCTCCTCGCCCGTGATTCACTGT   3019
                     |||||||||||||     ||||||||||||||||||||||||||||||||
Mutation         251 GTGCTTTGGAATA-----ACCACGGATGCTCCTCGCCCGTGATTCACTGT    295
gRNA                   GCTTTGGAATATCTTCACCA

Wildtype        3020 GATCTAAAGCCCAGTAACATCTTGTTGGATGAGGATATGGTTGCTCACCT   3069
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         296 GATCTAAAGCCCAGTAACATCTTGTTGGATGAGGATATGGTTGCTCACCT    345

Wildtype        3070 AGGTGACTTTGGTATTTCAAAACTGCTTGGTGAAGATGAGAGTGATTTAT   3119
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         346 AGGTGACTTTGGTATTTCAAAACTGCTTGGTGAAGATGAGAGTGATTTAT    395

Wildtype        3120 ACACAAAAACCTTAGCAACATTTGGTTATATTGCACCGGGTACGTCTCTT   3169
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         396 ACACAAAAACCTTAGCAACATTTGGTTATATTGCACCGGGTACGTCTCTT    445

Wildtype        3170 TGTTTTTGCTAATTTGAGCATTGATTTTTCATCTTTTT   3207
                     ||||||||||||||||||||||||||||||||||||||
Mutation         446 TGTTTTTGCTAATTTGAGCATTGATTTTTCATCTTTTT    483

Morphology : Serrated cotyledons