Plant Crispr Database

Plant Information

Name: CRT0138-1-4

Seed stock: 4969
Seed availability: No

Relationship:
Parent: CRT0138-1
Progeny: CRT0138-1-4-1

Transformation: CRT0138
transformation CRT0138 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0138-1-4 (T1 )	CRT0138	CTG0138-27	CGR0138-27	gene : Solyc08g082990 (Transporter gene)	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: AAAGAACGTAGTTATGTCGTTGCCACCAATCTGGTCTATATCGATAAACAACAAAGCCAGACAAGAACCCAACGATGATC CAGGTTGTGTAGTTCACAGCAGTGGCTGGTGGCATCATCCCTGTTGCTCCTATTAGCACTGGCATGTTAATCAGTTTGAT CCATTCTTGCTTAGGAAATGCCCTCGCTGCTAACCACACAATACCGGTGCTATCGCGCCAAATAAGAAGAACCAATTCAC CATCCCATAAGTTCCCAAGTCTCCGAAAATCCTTCTTGGTGCAATCAAACCCCAAATCACTGATGCATCATAAAACACAT GATCCGAAGGACAAGTCCACACCGTGTTGGACAACGTCTTGTCGCATATGTCTGGAATCGTTTCCATTAACCACCACGCT GTTCCCAAATACACAAACCCTGCTATTAGAGTACCTACTACCTGCAGTGTCCATTTTCTACTATTGTAAATACGTAAGAG AATCATCTATATTATCCATTATAGCGAGTAGCTTACCTGAGCCATAAACATTATTCTTGGAGGAATTTTCATGTAATGGC CAAGCTTGAAGTCTTGGAGGAAAGTTATAGCTTGAGTCATACTAATGTAACCATAGACCTTGAAGCACATGTTAGCGACC GGATATCCTGGATAAAGGTATCCTATGATATATTCCGTGATGACATTCAACCCTGGTGTCTGCAATGTTCAAAAATCATA TATATAAGTAATTAAGTGACTTTTCTGTTACAGATAGTTGATTGATCCTTGTTACCTGGTTGGTAATGGCGGTGATGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2322 TCATCACCGCCATTACCAACCAGGTAACAAGGATCAATCAACTATCTGTA 2371 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TCATCACCGCCATTACCAACCAGGTAACAAGGATCAATCAACTATCTGTA 50 Wildtype 2372 ACAGAAAAGTCACTTAATTACTTATATATATGATTTTTGAACATTGCAGA 2421 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 ACAGAAAAGTCACTTAATTACTTATATATATGATTTTTGAACATTGCAGA 100 Wildtype 2422 CACCAGGGTTGAATGTCATCACGGAATATATCATAGGATACCTTTATCCA 2471 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CACCAGGGTTGAATGTCATCACGGAATATATCATAGGATACCTTTATCCA 150 Wildtype 2472 GGATATCCGGTCGCTAACATGTGCTTCAAGGTCTATGGTTACATTAGTAT 2521 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GGATATCCGGTCGCTAACATGTGCTTCAAGGTCTATGGTTACATTAGTAT 200 Wildtype 2522 GACTCAAGCTATAACTTTCCTCCAAGACTTCAAGCTTGGCCATTACATGA 2571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GACTCAAGCTATAACTTTCCTCCAAGACTTCAAGCTTGGCCATTACATGA 250 Wildtype 2572 AAATTCCTCCAAGAATAATGTTTATGGCTCAGGTAAGCTACTCGCTATAA 2621 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 AAATTCCTCCAAGAATAATGTTTATGGCTCAGGTAAGCTACTCGCTATAA 300 Wildtype 2622 TGGATAATATAGATGATTCTCTTACGTATTTACAATAGTAGAAAATGGAC 2671 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 TGGATAATATAGATGATTCTCTTACGTATTTACAATAGTAGAAAATGGAC 350 Wildtype 2672 ACTGCAGGTAGTAGGTACTCTAATAGCAGGGTTTGTGTATTTGGGAACAG 2721 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 ACTGCAGGTAGTAGGTACTCTAATAGCAGGGTTTGTGTATTTGGGAACAG 400 Wildtype 2722 CGTGGTGGTTAATGGAAACGATTCCAGACATATGCGACAAGACGTTGTCC 2771 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 CGTGGTGGTTAATGGAAACGATTCCAGACATATGCGACAAGACGTTGTCC 450 Wildtype 2772 AACACGGTGTGGACTTGTCCTTCGGATCATGTGTTTTATGATGCATCAGT 2821 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 AACACGGTGTGGACTTGTCCTTCGGATCATGTGTTTTATGATGCATCAGT 500 Wildtype 2822 GATTTGGGGTTTGATTGCACCAAGAAGGATTTTCGGAGACTTGGGAACTT 2871 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 GATTTGGGGTTTGATTGCACCAAGAAGGATTTTCGGAGACTTGGGAACTT 550 Wildtype 2872 ATGGGATGGTGAATTGGTTCTTCTTATTTGGCGCGATAGCACCGGTACTT 2921 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| MutationRev 551 ATGGGATGGTGAATTGGTTCTTCTTATTTGGCGCGATAGCACCGGTA-TT 599 gRNA GTACTT Wildtype 2922 GTGTGGTTAGCAGCGAGGGCATTTCCTAAGCAAGAATGGATCAAACTGAT 2971 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 600 GTGTGGTTAGCAGCGAGGGCATTTCCTAAGCAAGAATGGATCAAACTGAT 649 gRNA GTACTTGTGTGGTTAGCAGC Wildtype 2972 TAACATGCCAGTGCTAATAGGAGCAACAGGGATGATGCCACCAGCCACTG 3021 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 650 TAACATGCCAGTGCTAATAGGAGCAACAGGGATGATGCCACCAGCCACTG 699 Wildtype 3022 CTGTGAACTACACAACCTGGATCATCGTTGGGTTCTTGTCTGGCTTTGTT 3071 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 700 CTGTGAACTACACAACCTGGATCATCGTTGGGTTCTTGTCTGGCTTTGTT 749 Wildtype 3072 GTTTATCGATATAGACCAGATTGGTGGCAACGACATAACTACGTTCTTT 3120 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 750 GTTTATCGATATAGACCAGATTGGTGGCAACGACATAACTACGTTCTTT 798	Morphology : Low germination (~10%); retarded growth

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0138-1-4 (T1 )

CRT0138

CTG0138-27

CGR0138-27

gene : Solyc08g082990 (Transporter gene)

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

AAAGAACGTAGTTATGTCGTTGCCACCAATCTGGTCTATATCGATAAACAACAAAGCCAGACAAGAACCCAACGATGATC
CAGGTTGTGTAGTTCACAGCAGTGGCTGGTGGCATCATCCCTGTTGCTCCTATTAGCACTGGCATGTTAATCAGTTTGAT
CCATTCTTGCTTAGGAAATGCCCTCGCTGCTAACCACACAATACCGGTGCTATCGCGCCAAATAAGAAGAACCAATTCAC
CATCCCATAAGTTCCCAAGTCTCCGAAAATCCTTCTTGGTGCAATCAAACCCCAAATCACTGATGCATCATAAAACACAT
GATCCGAAGGACAAGTCCACACCGTGTTGGACAACGTCTTGTCGCATATGTCTGGAATCGTTTCCATTAACCACCACGCT
GTTCCCAAATACACAAACCCTGCTATTAGAGTACCTACTACCTGCAGTGTCCATTTTCTACTATTGTAAATACGTAAGAG
AATCATCTATATTATCCATTATAGCGAGTAGCTTACCTGAGCCATAAACATTATTCTTGGAGGAATTTTCATGTAATGGC
CAAGCTTGAAGTCTTGGAGGAAAGTTATAGCTTGAGTCATACTAATGTAACCATAGACCTTGAAGCACATGTTAGCGACC
GGATATCCTGGATAAAGGTATCCTATGATATATTCCGTGATGACATTCAACCCTGGTGTCTGCAATGTTCAAAAATCATA
TATATAAGTAATTAAGTGACTTTTCTGTTACAGATAGTTGATTGATCCTTGTTACCTGGTTGGTAATGGCGGTGATGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2322 TCATCACCGCCATTACCAACCAGGTAACAAGGATCAATCAACTATCTGTA   2371
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TCATCACCGCCATTACCAACCAGGTAACAAGGATCAATCAACTATCTGTA     50

Wildtype        2372 ACAGAAAAGTCACTTAATTACTTATATATATGATTTTTGAACATTGCAGA   2421
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 ACAGAAAAGTCACTTAATTACTTATATATATGATTTTTGAACATTGCAGA    100

Wildtype        2422 CACCAGGGTTGAATGTCATCACGGAATATATCATAGGATACCTTTATCCA   2471
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 CACCAGGGTTGAATGTCATCACGGAATATATCATAGGATACCTTTATCCA    150

Wildtype        2472 GGATATCCGGTCGCTAACATGTGCTTCAAGGTCTATGGTTACATTAGTAT   2521
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GGATATCCGGTCGCTAACATGTGCTTCAAGGTCTATGGTTACATTAGTAT    200

Wildtype        2522 GACTCAAGCTATAACTTTCCTCCAAGACTTCAAGCTTGGCCATTACATGA   2571
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GACTCAAGCTATAACTTTCCTCCAAGACTTCAAGCTTGGCCATTACATGA    250

Wildtype        2572 AAATTCCTCCAAGAATAATGTTTATGGCTCAGGTAAGCTACTCGCTATAA   2621
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 AAATTCCTCCAAGAATAATGTTTATGGCTCAGGTAAGCTACTCGCTATAA    300

Wildtype        2622 TGGATAATATAGATGATTCTCTTACGTATTTACAATAGTAGAAAATGGAC   2671
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TGGATAATATAGATGATTCTCTTACGTATTTACAATAGTAGAAAATGGAC    350

Wildtype        2672 ACTGCAGGTAGTAGGTACTCTAATAGCAGGGTTTGTGTATTTGGGAACAG   2721
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 ACTGCAGGTAGTAGGTACTCTAATAGCAGGGTTTGTGTATTTGGGAACAG    400

Wildtype        2722 CGTGGTGGTTAATGGAAACGATTCCAGACATATGCGACAAGACGTTGTCC   2771
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 CGTGGTGGTTAATGGAAACGATTCCAGACATATGCGACAAGACGTTGTCC    450

Wildtype        2772 AACACGGTGTGGACTTGTCCTTCGGATCATGTGTTTTATGATGCATCAGT   2821
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 AACACGGTGTGGACTTGTCCTTCGGATCATGTGTTTTATGATGCATCAGT    500

Wildtype        2822 GATTTGGGGTTTGATTGCACCAAGAAGGATTTTCGGAGACTTGGGAACTT   2871
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 GATTTGGGGTTTGATTGCACCAAGAAGGATTTTCGGAGACTTGGGAACTT    550

Wildtype        2872 ATGGGATGGTGAATTGGTTCTTCTTATTTGGCGCGATAGCACCGGTACTT   2921
                     ||||||||||||||||||||||||||||||||||||||||||||||| ||
MutationRev      551 ATGGGATGGTGAATTGGTTCTTCTTATTTGGCGCGATAGCACCGGTA-TT    599
gRNA                                                             GTACTT

Wildtype        2922 GTGTGGTTAGCAGCGAGGGCATTTCCTAAGCAAGAATGGATCAAACTGAT   2971
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      600 GTGTGGTTAGCAGCGAGGGCATTTCCTAAGCAAGAATGGATCAAACTGAT    649
gRNA                 GTACTTGTGTGGTTAGCAGC

Wildtype        2972 TAACATGCCAGTGCTAATAGGAGCAACAGGGATGATGCCACCAGCCACTG   3021
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      650 TAACATGCCAGTGCTAATAGGAGCAACAGGGATGATGCCACCAGCCACTG    699

Wildtype        3022 CTGTGAACTACACAACCTGGATCATCGTTGGGTTCTTGTCTGGCTTTGTT   3071
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      700 CTGTGAACTACACAACCTGGATCATCGTTGGGTTCTTGTCTGGCTTTGTT    749

Wildtype        3072 GTTTATCGATATAGACCAGATTGGTGGCAACGACATAACTACGTTCTTT   3120
                     |||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      750 GTTTATCGATATAGACCAGATTGGTGGCAACGACATAACTACGTTCTTT    798

Morphology : Low germination (~10%); retarded growth