Plant Crispr Database

Plant Information

Name: CRT0138-19-2

Seed stock: 4973
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0138-19

Transformation: CRT0138
transformation CRT0138 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0138-19-2 (T1 )	CRT0138	CTG0138-12	CGR0138-12	gene : Solyc05g005910 (Peptide Transporter 1)	-1 bp/-1 bp Is tDNA present: No Mutation sequence: GTGTATTCATGATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGTCCCTTTGCTATCAAAATATACT GGACAGCCAAGAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCTGTATGTCTATGGCGTTGTCAGC AATAACAGAAAGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCAAGTGCGTTAGTGAACATGTCTG CTATGTGGCTCGTACCACAGTATGCACTACTCGGAGTGGTGAGGGTTCCCATTCTGTTGGACAGATTGAGTTCTTCTACT CTCTGTTACCCAAAAGCATGTCCAGCATGGCATCAGCTATGTACACTGTTGGGACTGCTGTGTCGAGTTTGGTTGTAAGT ATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAACTGGAGGCAAAACTAGCTGGCTTTCGAGCAACATTAATAGGGGTCA CCTAGATTACTATTTCTGGCTACTTACTTTTTTGAGTTTGCTCAACTTCTTCTATTTTCTGCTCATCTGCCGGTTATATG AACCTG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 175 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA 224 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA 50 Wildtype 225 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC 274 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC 100 Wildtype 275 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA 324 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA 150 Wildtype 325 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC 374 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC 200 Wildtype 375 AGTCCCAACAGTGTACATAGCTGATGCCATGCTGGACATGCTTTTGGGTA 424 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AGTCCCAACAGTGTACATAGCTGATGCCATGCTG---------------- 234 Wildtype 425 ACAGAGAGTAGAAGAACTCAATCTGTCCAACAGAATGGGAACCCTCAGCC 474 MutationRev 234 -------------------------------------------------- 234 gRNA TCAGCC Wildtype 475 ACTCCGAGTAGTGCATACTGTGGTACGAGCCACATAGCAGACATGTTCAC 524 \|\|\|\|\|\|\|\|\|\|\| MutationRev 235 ---------------------------------------GACATGTTCAC 245 gRNA TCAGCCACTCCGAGTAGTGC Wildtype 525 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC 574 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 246 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC 295 Wildtype 575 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT 624 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 296 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT 345 Wildtype 625 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT 674 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 346 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT 395 Wildtype 675 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA 724 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 396 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA 445 Wildtype 725 TTGTTAAAGCAATAATC 741 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 446 TTGTTAAAGCAATAATC 462	Morphology : Stunted growth; very branchy; smaller; Germination ~60%
CRT0138-19-2 (T1 )	CRT0138	CTG0138-14	CGR0138-14	gene : Solyc05g005940 (Solute Transporter 1)	5 nucleotides substitution Is tDNA present: No Mutation sequence: TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAATA TAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAGCC AGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTC CCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCC TCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGGTCGTCCTCATG CCCTTCTGTGATTGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACC CAATTCCCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCG TAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 149 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT 198 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT 50 Wildtype 199 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA 248 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA 100 Wildtype 249 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG 298 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG 150 Wildtype 299 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT 348 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT 200 Wildtype 349 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGTGT 398 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGT-- 248 Wildtype 399 ACATAGCTGACGCGATGCTTGCCATGCTTTTCGGTAATAGAGTGTAGAAA 448 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 249 ------CTG---------TTGCCATGCTTTTCGGTAATAGAGTGTAGAAA 283 Wildtype 449 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC 498 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 284 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC 333 Wildtype 499 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT 548 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 334 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT 383 Wildtype 549 CGTCTTCATGCCCTTCCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTT 598 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 384 CGTCTTCATGCCCTTCC------GCCCTTTTTCGTCTTATGCTTTCTGTT 427 gRNA TTCCGCTATTGCCCTTTTTC Wildtype 599 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT 648 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 428 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT 477 Wildtype 649 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA 698 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 478 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA 527 Wildtype 699 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA 748 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 528 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA 577 Wildtype 749 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT 798 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 578 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT 627 Wildtype 799 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA 848 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 628 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA 677 Wildtype 849 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA 898 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 678 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA 727 Wildtype 899 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC 948 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 728 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC 777 Wildtype 949 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC 998 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 778 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC 827 Wildtype 999 AAGCTCTAT 1007 \|\|\|\|\|\|\|\|\| Mutation 828 AAGCTCTAT 836	Morphology : Stunted growth; very branchy; smaller; Germination ~60%

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0138-19-2 (T1 )

CRT0138

CTG0138-12

CGR0138-12

gene : Solyc05g005910 (Peptide Transporter 1)

-1 bp/-1 bp

Is tDNA present: No

Mutation sequence:

GTGTATTCATGATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGTCCCTTTGCTATCAAAATATACT
GGACAGCCAAGAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCTGTATGTCTATGGCGTTGTCAGC
AATAACAGAAAGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCAAGTGCGTTAGTGAACATGTCTG
CTATGTGGCTCGTACCACAGTATGCACTACTCGGAGTGGTGAGGGTTCCCATTCTGTTGGACAGATTGAGTTCTTCTACT
CTCTGTTACCCAAAAGCATGTCCAGCATGGCATCAGCTATGTACACTGTTGGGACTGCTGTGTCGAGTTTGGTTGTAAGT
ATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAACTGGAGGCAAAACTAGCTGGCTTTCGAGCAACATTAATAGGGGTCA
CCTAGATTACTATTTCTGGCTACTTACTTTTTTGAGTTTGCTCAACTTCTTCTATTTTCTGCTCATCTGCCGGTTATATG
AACCTG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         175 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA    224
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA     50

Wildtype         225 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC    274
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC    100

Wildtype         275 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA    324
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA    150

Wildtype         325 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC    374
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC    200

Wildtype         375 AGTCCCAACAGTGTACATAGCTGATGCCATGCTGGACATGCTTTTGGGTA    424
                     ||||||||||||||||||||||||||||||||||                
MutationRev      201 AGTCCCAACAGTGTACATAGCTGATGCCATGCTG----------------    234

Wildtype         425 ACAGAGAGTAGAAGAACTCAATCTGTCCAACAGAATGGGAACCCTCAGCC    474
                                                                       
MutationRev      234 --------------------------------------------------    234
gRNA                                                             TCAGCC

Wildtype         475 ACTCCGAGTAGTGCATACTGTGGTACGAGCCACATAGCAGACATGTTCAC    524
                                                            |||||||||||
MutationRev      235 ---------------------------------------GACATGTTCAC    245
gRNA                 TCAGCCACTCCGAGTAGTGC

Wildtype         525 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC    574
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      246 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC    295

Wildtype         575 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT    624
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      296 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT    345

Wildtype         625 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT    674
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      346 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT    395

Wildtype         675 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA    724
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      396 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA    445

Wildtype         725 TTGTTAAAGCAATAATC    741
                     |||||||||||||||||
MutationRev      446 TTGTTAAAGCAATAATC    462

Morphology : Stunted growth; very branchy; smaller; Germination ~60%

CRT0138-19-2 (T1 )

CRT0138

CTG0138-14

CGR0138-14

gene : Solyc05g005940 (Solute Transporter 1)

5 nucleotides substitution

Is tDNA present: No

Mutation sequence:

TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAATA
TAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAGCC
AGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTC
CCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCC
TCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGGTCGTCCTCATG
CCCTTCTGTGATTGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACC
CAATTCCCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCG
TAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT
ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         149 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT    198
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT     50

Wildtype         199 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA    248
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA    100

Wildtype         249 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG    298
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG    150

Wildtype         299 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT    348
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT    200

Wildtype         349 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGTGT    398
                     ||||||||||||||||||||||||||||||||||||||||||||||||  
Mutation         201 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGT--    248

Wildtype         399 ACATAGCTGACGCGATGCTTGCCATGCTTTTCGGTAATAGAGTGTAGAAA    448
                           |||         ||||||||||||||||||||||||||||||||
Mutation         249 ------CTG---------TTGCCATGCTTTTCGGTAATAGAGTGTAGAAA    283

Wildtype         449 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC    498
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         284 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC    333

Wildtype         499 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT    548
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         334 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT    383

Wildtype         549 CGTCTTCATGCCCTTCCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTT    598
                     |||||||||||||||||      |||||||||||||||||||||||||||
Mutation         384 CGTCTTCATGCCCTTCC------GCCCTTTTTCGTCTTATGCTTTCTGTT    427
gRNA                              TTCCGCTATTGCCCTTTTTC

Wildtype         599 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT    648
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         428 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT    477

Wildtype         649 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA    698
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         478 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA    527

Wildtype         699 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA    748
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         528 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA    577

Wildtype         749 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT    798
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         578 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT    627

Wildtype         799 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA    848
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         628 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA    677

Wildtype         849 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA    898
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         678 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA    727

Wildtype         899 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC    948
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         728 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC    777

Wildtype         949 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC    998
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         778 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC    827

Wildtype         999 AAGCTCTAT   1007
                     |||||||||
Mutation         828 AAGCTCTAT    836

Morphology : Stunted growth; very branchy; smaller; Germination ~60%