Is tDNA present: No

Mutation sequence:

CTGACACTTTTATCACAAGTTCTTATACTTGCTCACTTACTCATCAGTGAGGAATTCAAGATTATACGGCTTATTCATGT
TTTTCATCTTTAGAACTTGTTTAGTTGTAGAGGCTTATTCCTTTTTATCTCAACAGGGAACGTGTATTCTTTGGCTCACC
ACAATAATGCCACAACTCCGGCCTACCTTGCGGTCAGCATCAACATGTTTGTGATGGAACAACAGCAGCCCAACTTGCTA
TACTTTTGTGTTCTTTGGGGCTTATGTCCATTGGTACTGGTTTTAACAGACCTTGTTCCATAGCATTTGGTGCTGACCAA
TTGGAGAACAAAGAAAATCCAGATAACGAGAGGCTTATAGATAGCTATTTCAACTGGTATTATGCCAGTGTAGGAGTTTC
ACTTGTTCTTGCAGTTACTGTAATTGTTTATATCCAAGACCATTTTGGTTGGCAAATTGGGTTTGGTGTCCCTGCTTTGC
TCATGCTTATATCTGTTTCCATTTTCTTGATCGGATCTCCTCTTTATATCAAAGCAAAAGCTAGAGGGAGTTTGTTCACT
GGATTGTTTCAAGTAGCAGTGGCAGCCTTCAGAAAAAGACATATCAATGTTGAGTCAAATTATATTGCTGACTGCTACTA
TAAAGAACCTGAATCGAACCTGCTCGAACCATCTGCCGACTTCAGGTATAGGTGCCTCCATCTTTTCTTCAATGGTATAG
ACATCTTCAACATCCATTCATGCACTTAGTATCTTGCAATACTAATTTGTTATACTGTATTACATGATGAACTCGTGTGA
T

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1633 ATCACACGAGTTCATCATGTAATACAGTATAACAAATTAGTATTGCAAGA   1682
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ATCACACGAGTTCATCATGTAATACAGTATAACAAATTAGTATTGCAAGA     50

Wildtype        1683 TACTAAGTGCATGAATGGATGTTGAAGATGTCTATACCATTGAAGAAAAG   1732
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TACTAAGTGCATGAATGGATGTTGAAGATGTCTATACCATTGAAGAAAAG    100

Wildtype        1733 ATGGAGGCACCTATACCTGAAGTCGGCAGATGGTTCGAGCAGGTTCGATT   1782
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATGGAGGCACCTATACCTGAAGTCGGCAGATGGTTCGAGCAGGTTCGATT    150

Wildtype        1783 CAGGTTCTTTATAGTAGCAGTCAGCAATATAATTTGACTCAACATTGATA   1832
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 CAGGTTCTTTATAGTAGCAGTCAGCAATATAATTTGACTCAACATTGATA    200

Wildtype        1833 TGTCTTTTTCTGAAGGCTGCCACTGCTACTTGAAACAATCCAGTGAACAA   1882
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TGTCTTTTTCTGAAGGCTGCCACTGCTACTTGAAACAATCCAGTGAACAA    250

Wildtype        1883 ACTCCCTCTAGCTTTTGCTTTGATATAAAGAGGAGATCCGATCAAGAAAA   1932
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 ACTCCCTCTAGCTTTTGCTTTGATATAAAGAGGAGATCCGATCAAGAAAA    300

Wildtype        1933 TGGAAACAGATATAAGCATGAGCAAAGCAGGGACACCAAACCCAATTTGC   1982
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TGGAAACAGATATAAGCATGAGCAAAGCAGGGACACCAAACCCAATTTGC    350

Wildtype        1983 CAACCAAAATGGTCTTGGATATAAACAATTACAGTAACTGCAAGAACAAG   2032
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 CAACCAAAATGGTCTTGGATATAAACAATTACAGTAACTGCAAGAACAAG    400

Wildtype        2033 TGAAACTCCTACACTGGCATAATACCAGTTGAAATAGCTATCTATAAGCC   2082
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 TGAAACTCCTACACTGGCATAATACCAGTTGAAATAGCTATCTATAAGCC    450

Wildtype        2083 TCTCGTTATCTGGATTTTCTTTGTTCTCCAATTGGTCAGCACCAAATGCT   2132
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 TCTCGTTATCTGGATTTTCTTTGTTCTCCAATTGGTCAGCACCAAATGCT    500

Wildtype        2133 ATGGAACAAGGTCTGTTAAAACCAGTACCAATGGACATAAGCCCCAAAGA   2182
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 ATGGAACAAGGTCTGTTAAAACCAGTACCAATGGACATAAGCCCCAAAGA    550

Wildtype        2183 ACACAAAAGTATAGCAAGTTGGGCTGCTGTTGTTCCATCACAAACATGTT   2232
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      551 ACACAAAAGTATAGCAAGTTGGGCTGCTGTTGTTCCATCACAAACATGTT    600

Wildtype        2233 GATGCTGACCGCAAGGTAAAGGCCGGAGTTGTGGCATTATTGTGGTGAGC   2282
                     |||||||||||||||||  |||||||||||||||||||||||||||||||
MutationRev      601 GATGCTGACCGCAAGGT--AGGCCGGAGTTGTGGCATTATTGTGGTGAGC    648
gRNA                    GCTGACCGCAAGGTAAAGGC

Wildtype        2283 CAAAGAATACACGTTCCCTGTTGAGATAAAAAGGAATAAGCCTCTACAAC   2332
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      649 CAAAGAATACACGTTCCCTGTTGAGATAAAAAGGAATAAGCCTCTACAAC    698

Wildtype        2333 TAAACAAGTTCTAAAGATGAAAAACATGAATAAGCCGTATAATCTTGAAT   2382
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      699 TAAACAAGTTCTAAAGATGAAAAACATGAATAAGCCGTATAATCTTGAAT    748

Wildtype        2383 TCCTCACTGATGAGTAAGTGAGCAAGTATAAGAACTTGTGATAAAAGTGT   2432
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      749 TCCTCACTGATGAGTAAGTGAGCAAGTATAAGAACTTGTGATAAAAGTGT    798

Wildtype        2433 CAG   2435
                     |||
MutationRev      799 CAG    801

Is tDNA present: No

Mutation sequence:

ATTCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAA
TATAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAG
CCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAG
TCCCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAG
CCTCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGATCGTCTTCA
TGCCCTTCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAAC
CCAATTCCCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATC
GTAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGA
TATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCATGTCTACAAACATCATAAAACCAGTAGAC
CACATAGGAAGTATTCTAATAAGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATCCGAAACTGA
TCCATCAGGATTCAAATCTCTTTCAGGATCTTC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         149 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT    198
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT     50

Wildtype         199 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA    248
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA    100

Wildtype         249 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG    298
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG    150

Wildtype         299 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT    348
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT    200

Wildtype         349 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGTGT    398
                     ||||||||||||||||||||||||||||||||||||||||||||||||  
Mutation         201 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGT--    248

Wildtype         399 ACATAGCTGACGCGATGCTTGCCATGCTTTTCGGTAATAGAGTGTAGAAA    448
                           |||         ||||||||||||||||||||||||||||||||
Mutation         249 ------CTG---------TTGCCATGCTTTTCGGTAATAGAGTGTAGAAA    283

Wildtype         449 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC    498
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         284 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC    333

Wildtype         499 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT    548
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         334 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT    383

Wildtype         549 CGTCTTCATGCCCTTCCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTT    598
                     |||||||||||||||||      |||||||||||||||||||||||||||
Mutation         384 CGTCTTCATGCCCTTCC------GCCCTTTTTCGTCTTATGCTTTCTGTT    427
gRNA                              TTCCGCTATTGCCCTTTTTC

Wildtype         599 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT    648
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         428 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT    477

Wildtype         649 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA    698
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         478 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA    527

Wildtype         699 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA    748
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         528 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA    577

Wildtype         749 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT    798
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         578 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT    627

Wildtype         799 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA    848
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         628 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA    677

Wildtype         849 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA    898
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         678 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA    727

Wildtype         899 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC    948
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         728 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC    777

Wildtype         949 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC    998
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         778 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC    827

Wildtype         999 AAGCTCTAT   1007
                     |||||||||
Mutation         828 AAGCTCTAT    836