Plant Crispr Database

Plant Information

Name: CRT0138-28-2

Seed stock: 4974
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0138-28

Transformation: CRT0138
transformation CRT0138 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0138-28-2 (T1 )	CRT0138	CTG0138-14	CGR0138-14	gene : Solyc05g005940 (Solute Transporter 1)	-6 bp/-6 bp Is tDNA present: Yes Mutation sequence: TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAATA TAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAGCC AGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTC CCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCC TCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGATCGTCTTCATG CCCTTCCGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTC CCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCGTAGAAA GTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGATATGTCT ATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCATGTCTACAAACATCATAAAACCAGTAGACCACATAG GAAGTATTCTAATAAGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATCCGAAACTGATCCATCA GGATTCAAATCTCTTTCAGGATCTTCAATCATACAAGCTCTAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 149 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT 198 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT 50 Wildtype 199 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA 248 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA 100 Wildtype 249 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG 298 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG 150 Wildtype 299 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT 348 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT 200 Wildtype 349 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGTGT 398 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGT-- 248 Wildtype 399 ACATAGCTGACGCGATGCTTGCCATGCTTTTCGGTAATAGAGTGTAGAAA 448 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 249 ------CTG---------TTGCCATGCTTTTCGGTAATAGAGTGTAGAAA 283 Wildtype 449 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC 498 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 284 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC 333 Wildtype 499 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT 548 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 334 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT 383 Wildtype 549 CGTCTTCATGCCCTTCCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTT 598 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 384 CGTCTTCATGCCCTTCC------GCCCTTTTTCGTCTTATGCTTTCTGTT 427 gRNA TTCCGCTATTGCCCTTTTTC Wildtype 599 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT 648 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 428 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT 477 Wildtype 649 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA 698 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 478 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA 527 Wildtype 699 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA 748 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 528 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA 577 Wildtype 749 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT 798 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 578 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT 627 Wildtype 799 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA 848 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 628 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA 677 Wildtype 849 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA 898 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 678 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA 727 Wildtype 899 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC 948 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 728 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC 777 Wildtype 949 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC 998 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 778 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC 827 Wildtype 999 AAGCTCTAT 1007 \|\|\|\|\|\|\|\|\| Mutation 828 AAGCTCTAT 836
CRT0138-28-2 (T1 )	CRT0138	CTG0138-26	CGR0138-26	gene : Solyc06g084140 (Transporter gene)	-2 bp/-2 bp Is tDNA present: Yes Mutation sequence: AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCAATATTTATTTCCAATTTTTGAATGGGGTTC AAGATACAGTTTTGGTTTCTTCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCTCAAGGAATAA GCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACTATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAG GTAAAAAATTTATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCCACCATTAGTGTACGCATTAA TGGGAAGTTCAAGAGATTTAGCAGTGGGAACCGTTTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTGAA TCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCACTTTCTTCACCGGGTTATTCGAACTAGCACTTG GATTTTTCAGGTACGTAATGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTATTTTTAGGCTGGG ATTTATAGTGGATTTTCTATCACATGCAACCATTGTGGGATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAAT TAAAAGGAATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTTACGTTCTGTATTTGCACAAACG CACGCGGTACG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 224 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA 273 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA 50 Wildtype 274 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT 323 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT 100 Wildtype 324 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT 373 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT 150 Wildtype 374 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT 423 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT 200 Wildtype 424 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT 473 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT 250 Wildtype 474 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC 523 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC 300 Wildtype 524 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA 573 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 301 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA 350 gRNA GCAGTGGGAA Wildtype 574 CCGTGGCTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG 623 \|\|\|\| .\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 351 CCGT--TTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG 398 gRNA GCAGTGGGAACCGTGGCTGT Wildtype 624 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC 673 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 399 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC 448 Wildtype 674 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA 723 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 449 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA 498 Wildtype 724 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT 773 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 499 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT 548 Wildtype 774 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG 823 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 549 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG 598 Wildtype 824 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA 873 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 599 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA 648 Wildtype 874 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT 923 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 649 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT 698 Wildtype 924 ACGTTCTGTATTTGCACAAACGCACGCGGTACG 956 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 699 ACGTTCTGTATTTGCACAAACGCACGCGGTACG 731

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0138-28-2 (T1 )

CRT0138

CTG0138-14

CGR0138-14

gene : Solyc05g005940 (Solute Transporter 1)

-6 bp/-6 bp

Is tDNA present: Yes

Mutation sequence:

TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAATA
TAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAGCC
AGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTC
CCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCC
TCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGATCGTCTTCATG
CCCTTCCGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTC
CCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCGTAGAAA
GTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGATATGTCT
ATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCATGTCTACAAACATCATAAAACCAGTAGACCACATAG
GAAGTATTCTAATAAGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATCCGAAACTGATCCATCA
GGATTCAAATCTCTTTCAGGATCTTCAATCATACAAGCTCTAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         149 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT    198
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGT     50

Wildtype         199 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA    248
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATCATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCA    100

Wildtype         249 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG    298
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTG    150

Wildtype         299 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT    348
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACT    200

Wildtype         349 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGTGT    398
                     ||||||||||||||||||||||||||||||||||||||||||||||||  
Mutation         201 ACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGT--    248

Wildtype         399 ACATAGCTGACGCGATGCTTGCCATGCTTTTCGGTAATAGAGTGTAGAAA    448
                           |||         ||||||||||||||||||||||||||||||||
Mutation         249 ------CTG---------TTGCCATGCTTTTCGGTAATAGAGTGTAGAAA    283

Wildtype         449 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC    498
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         284 AACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGC    333

Wildtype         499 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT    548
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         334 ATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGAT    383

Wildtype         549 CGTCTTCATGCCCTTCCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTT    598
                     |||||||||||||||||      |||||||||||||||||||||||||||
Mutation         384 CGTCTTCATGCCCTTCC------GCCCTTTTTCGTCTTATGCTTTCTGTT    427
gRNA                              TTCCGCTATTGCCCTTTTTC

Wildtype         599 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT    648
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         428 ATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCAT    477

Wildtype         649 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA    698
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         478 TCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCA    527

Wildtype         699 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA    748
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         528 AAGGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATA    577

Wildtype         749 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT    798
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         578 ATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT    627

Wildtype         799 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA    848
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         628 ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCA    677

Wildtype         849 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA    898
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         678 TGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATA    727

Wildtype         899 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC    948
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         728 AGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATC    777

Wildtype         949 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC    998
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         778 CGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATAC    827

Wildtype         999 AAGCTCTAT   1007
                     |||||||||
Mutation         828 AAGCTCTAT    836

CRT0138-28-2 (T1 )

CRT0138

CTG0138-26

CGR0138-26

gene : Solyc06g084140 (Transporter gene)

-2 bp/-2 bp

Is tDNA present: Yes

Mutation sequence:

AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCAATATTTATTTCCAATTTTTGAATGGGGTTC
AAGATACAGTTTTGGTTTCTTCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCTCAAGGAATAA
GCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACTATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAG
GTAAAAAATTTATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCCACCATTAGTGTACGCATTAA
TGGGAAGTTCAAGAGATTTAGCAGTGGGAACCGTTTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTGAA
TCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCACTTTCTTCACCGGGTTATTCGAACTAGCACTTG
GATTTTTCAGGTACGTAATGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTATTTTTAGGCTGGG
ATTTATAGTGGATTTTCTATCACATGCAACCATTGTGGGATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAAT
TAAAAGGAATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTTACGTTCTGTATTTGCACAAACG
CACGCGGTACG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         224 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA    273
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA     50

Wildtype         274 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT    323
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT    100

Wildtype         324 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT    373
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT    150

Wildtype         374 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT    423
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT    200

Wildtype         424 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT    473
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT    250

Wildtype         474 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC    523
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC    300

Wildtype         524 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA    573
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA    350
gRNA                                                         GCAGTGGGAA

Wildtype         574 CCGTGGCTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG    623
                     ||||  .|||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CCGT--TTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG    398
gRNA                 GCAGTGGGAACCGTGGCTGT

Wildtype         624 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC    673
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         399 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC    448

Wildtype         674 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA    723
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         449 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA    498

Wildtype         724 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT    773
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         499 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT    548

Wildtype         774 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG    823
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         549 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG    598

Wildtype         824 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA    873
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         599 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA    648

Wildtype         874 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT    923
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         649 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT    698

Wildtype         924 ACGTTCTGTATTTGCACAAACGCACGCGGTACG    956
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Mutation         699 ACGTTCTGTATTTGCACAAACGCACGCGGTACG    731