Plant Crispr Database

Plant Information

Name: CRT0138-32-11

Seed stock: 4976
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0138-32
Progeny: CRT0138-32-11-15

Transformation: CRT0138
transformation CRT0138 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0138-32-11 (T1 )	CRT0138	CTG0138-3	CGR0138-3	gene : Solyc03g005820 (Permease Transporter )	-2 bp/-2 bp Is tDNA present: Yes Mutation sequence: TGCCACAACATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTCCCTAATTGGTACTTTTCCATCT CCATGCTTAAATTCTTCCAATCTCCACTTATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAATT GACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGATACGAAAATTCCGTTAAAGAATACATCAAGGA CCCACCAATCGTAGAAAAAAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCACTCGTTATTATGAACCACAAGAATG ACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACTATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGAT AATACTAAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGTACTTTAGACCGATCGAGTTGAAAA CACTAACCCCACCAAGTAACACACCTAGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCCTTTG ATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGAAAGGAAGAAGAATTGGGAACCCTGCATTTTGCAC TAAGGAGACTAACCATATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTACTACTTCTCCAATTA TAGTAAAAACGGTAAAAATGGCAACTTGGTACCACCATTTATGCTTGGCAAAGTAGGAACAACAATTAGTTGATCCACTT GATTCAATGTTGGAATTTTCATCCTCACATATTTCTTCATCTGTCAAAAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 392 CATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTC 441 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 CATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTC 50 Wildtype 442 CCTAATTGGTACTTTTCCATCTCCATGCTTAAATTCTTCCAATCTCCACT 491 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 CCTAATTGGTACTTTTCCATCTCCATGCTTAAATTCTTCCAATCTCCACT 100 Wildtype 492 TATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAA 541 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAA 150 Wildtype 542 TTGACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGA 591 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 TTGACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGA 200 Wildtype 592 TACGAAAATTCCGTTAAAGAATACATCAAGGACCCACCAATCGTAGAAAA 641 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 TACGAAAATTCCGTTAAAGAATACATCAAGGACCCACCAATCGTAGAAAA 250 Wildtype 642 AAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCAGACTCGTTATTAT 691 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Mutation 251 AAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCAG-------ATTAT 293 gRNA CAGACTCGTTATTAT Wildtype 692 GAACCACAAGAATGACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACT 741 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 294 GAACCACAAGAATGACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACT 343 gRNA CAGACTCGTTATTATGAACC Wildtype 742 ATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGATAATACT 791 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 344 ATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGATAATACT 393 Wildtype 792 AAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGT 841 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 394 AAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGT 443 Wildtype 842 ACTTTAGACCGATCGAGTTGAAAACACTAACCCCACCAAGTAACACACCT 891 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 444 ACTTTAGACCGATCGAGTTGAAAACACTAACCCCACCAAGTAACACACCT 493 Wildtype 892 AGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCC 941 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 494 AGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCC 543 Wildtype 942 TTTGATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGA 991 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 544 TTTGATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGA 593 Wildtype 992 AAGGAAGAAGAATTGGGAACCCTGCATTTTGCACTAAGGAGACTAACCAT 1041 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 594 AAGGAAGAAGAATTGGGAACCCTGCATTTTGCACTAAGGAGACTAACCAT 643 Wildtype 1042 ATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTAC 1091 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 644 ATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTAC 693 Wildtype 1092 TACTTCTCCAATTATAGTAAAAACGGTAAAAATGGCAACTTGGTACCACC 1141 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 694 TACTTCTCCAATTATAGTAAAAACGGTAAAAATGGCAACTTGGTACCACC 743 Wildtype 1142 ATTTA 1146 \|\|\|\|\| Mutation 744 ATTTA 748

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0138-32-11 (T1 )

CRT0138

CTG0138-3

CGR0138-3

gene : Solyc03g005820 (Permease Transporter )

-2 bp/-2 bp

Is tDNA present: Yes

Mutation sequence:

TGCCACAACATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTCCCTAATTGGTACTTTTCCATCT
CCATGCTTAAATTCTTCCAATCTCCACTTATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAATT
GACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGATACGAAAATTCCGTTAAAGAATACATCAAGGA
CCCACCAATCGTAGAAAAAAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCACTCGTTATTATGAACCACAAGAATG
ACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACTATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGAT
AATACTAAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGTACTTTAGACCGATCGAGTTGAAAA
CACTAACCCCACCAAGTAACACACCTAGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCCTTTG
ATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGAAAGGAAGAAGAATTGGGAACCCTGCATTTTGCAC
TAAGGAGACTAACCATATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTACTACTTCTCCAATTA
TAGTAAAAACGGTAAAAATGGCAACTTGGTACCACCATTTATGCTTGGCAAAGTAGGAACAACAATTAGTTGATCCACTT
GATTCAATGTTGGAATTTTCATCCTCACATATTTCTTCATCTGTCAAAAG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         392 CATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTC    441
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTC     50

Wildtype         442 CCTAATTGGTACTTTTCCATCTCCATGCTTAAATTCTTCCAATCTCCACT    491
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CCTAATTGGTACTTTTCCATCTCCATGCTTAAATTCTTCCAATCTCCACT    100

Wildtype         492 TATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAA    541
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAA    150

Wildtype         542 TTGACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGA    591
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TTGACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGA    200

Wildtype         592 TACGAAAATTCCGTTAAAGAATACATCAAGGACCCACCAATCGTAGAAAA    641
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TACGAAAATTCCGTTAAAGAATACATCAAGGACCCACCAATCGTAGAAAA    250

Wildtype         642 AAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCAGACTCGTTATTAT    691
                     ||||||||||||||||||||||||||||||||||||||       |||||
Mutation         251 AAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCAG-------ATTAT    293
gRNA                                                    CAGACTCGTTATTAT

Wildtype         692 GAACCACAAGAATGACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACT    741
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         294 GAACCACAAGAATGACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACT    343
gRNA                 CAGACTCGTTATTATGAACC

Wildtype         742 ATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGATAATACT    791
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         344 ATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGATAATACT    393

Wildtype         792 AAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGT    841
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         394 AAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGT    443

Wildtype         842 ACTTTAGACCGATCGAGTTGAAAACACTAACCCCACCAAGTAACACACCT    891
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         444 ACTTTAGACCGATCGAGTTGAAAACACTAACCCCACCAAGTAACACACCT    493

Wildtype         892 AGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCC    941
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         494 AGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCC    543

Wildtype         942 TTTGATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGA    991
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         544 TTTGATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGA    593

Wildtype         992 AAGGAAGAAGAATTGGGAACCCTGCATTTTGCACTAAGGAGACTAACCAT   1041
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         594 AAGGAAGAAGAATTGGGAACCCTGCATTTTGCACTAAGGAGACTAACCAT    643

Wildtype        1042 ATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTAC   1091
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         644 ATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTAC    693

Wildtype        1092 TACTTCTCCAATTATAGTAAAAACGGTAAAAATGGCAACTTGGTACCACC   1141
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         694 TACTTCTCCAATTATAGTAAAAACGGTAAAAATGGCAACTTGGTACCACC    743

Wildtype        1142 ATTTA   1146
                     |||||
Mutation         744 ATTTA    748