Is tDNA present: No

Mutation sequence:

TGCCACAACATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTCCCTAATTGGTACTTTTCCATCT
CCATGCTTAAATTCTTCCAATCTCCACTTATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAATT
GACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGATACGAAAATTCCGTTAAAGAATACATCAAGGA
CCCACCAATCGTAGAAAAAAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCACTCGTTATTATGAACCACAAGAATG
ACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACTATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGAT
AATACTAAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGTACTTTAGACCGATCGAGTTGAAAA
CACTAACCCCACCAAGTAACACACCTAGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCCTTTG
ATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGAAAGGAAGAAGAATTGGGAACCCTGCATTTTGCAC
TAAGGAGACTAACCATATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTACTACTTCTCCAATTA
TAGTAAAAACGGTAAAAATGGCAACTTGGTACCACCATTTATGCTTGGCAAAGTAGGAACAACAATTAGTTGATCCACTT
GATTCAATGTTGGAATTTTCATCCTCACATATTTCTTCATCTGTCAAAAG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         392 CATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTC    441
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CATTTAATTGACATGACATGGCTATAAAAGACAAGATGATGACTAATTTC     50

Wildtype         442 CCTAATTGGTACTTTTCCATCTCCATGCTTAAATTCTTCCAATCTCCACT    491
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CCTAATTGGTACTTTTCCATCTCCATGCTTAAATTCTTCCAATCTCCACT    100

Wildtype         492 TATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAA    541
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TATAAAAAAACCTAGTAGGATCACTATGCTTGCAACTAGGGTTTGGTAAA    150

Wildtype         542 TTGACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGA    591
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TTGACATCTCTATAATATCTATGAACTCCCTAGCCTTTATTATTTTTCGA    200

Wildtype         592 TACGAAAATTCCGTTAAAGAATACATCAAGGACCCACCAATCGTAGAAAA    641
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TACGAAAATTCCGTTAAAGAATACATCAAGGACCCACCAATCGTAGAAAA    250

Wildtype         642 AAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCAGACTCGTTATTAT    691
                     ||||||||||||||||||||||||||||||||||||||       |||||
Mutation         251 AAACCCTAGTAAAAACTTTCCGCTAGACGTTTCCCCAG-------ATTAT    293
gRNA                                                    CAGACTCGTTATTAT

Wildtype         692 GAACCACAAGAATGACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACT    741
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         294 GAACCACAAGAATGACTGAAGCAGCTGTTACAAGAACTAGAGAGTTTACT    343
gRNA                 CAGACTCGTTATTATGAACC

Wildtype         742 ATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGATAATACT    791
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         344 ATAACTGGGGTGAACTTATGTGCATTGTGGAATAAAGAGAAGATAATACT    393

Wildtype         792 AAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGT    841
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         394 AAACCCTAGTCGAGTGGTACTAGTGAGAGAATGTGTTGATACTTGAAGGT    443

Wildtype         842 ACTTTAGACCGATCGAGTTGAAAACACTAACCCCACCAAGTAACACACCT    891
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         444 ACTTTAGACCGATCGAGTTGAAAACACTAACCCCACCAAGTAACACACCT    493

Wildtype         892 AGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCC    941
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         494 AGAACAGCATAAACTAAAAAGATTATGCAAATATGGGGTTCTTGATTTCC    543

Wildtype         942 TTTGATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGA    991
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         544 TTTGATCACCTCAATTTGGCTATGATTTTTGAGTGACACAGTGTATAGGA    593

Wildtype         992 AAGGAAGAAGAATTGGGAACCCTGCATTTTGCACTAAGGAGACTAACCAT   1041
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         594 AAGGAAGAAGAATTGGGAACCCTGCATTTTGCACTAAGGAGACTAACCAT    643

Wildtype        1042 ATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTAC   1091
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         644 ATACTTTTACCACCTTTGGTAAAATATGTTTTAACCAATAGTGATGTTAC    693

Wildtype        1092 TACTTCTCCAATTATAGTAAAAACGGTAAAAATGGCAACTTGGTACCACC   1141
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         694 TACTTCTCCAATTATAGTAAAAACGGTAAAAATGGCAACTTGGTACCACC    743

Wildtype        1142 ATTTA   1146
                     |||||
Mutation         744 ATTTA    748