Plant Crispr Database

Plant Information

Name: CRT0138-6-11

Seed stock: 4970
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0138-6

Transformation: CRT0138
transformation CRT0138 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0138-6-11 (T1 )	CRT0138	CTG0138-16	CGR0138-16	gene : Solyc05g005950 (Solute Transporter 2)	-3 bp/-3 bp Is tDNA present: No Mutation sequence: GACCATGCCATTCATCATTTGTACTACTCTCACTCTACTCTTTTACTTCAAATTGTTTTGCAATGTGTTAATTCTAATAT CAATTTTTTGTTATTGTTGTTGCTATTGTTTTCAGTGAATGAATCATTTGAAAGGATAGCAAGTTATGGTTCACAAACAA ACATGATAATTTATTTGATGACATATTATAACATGAGTGCTGCTACTGGCACTAGTATTGGTATATGGGGTGCACTTTCA AGTGGATTGGCTATTGTTGGGGCGATTATTGCTGATTGTTATTGGGGTCGATATAATGCTGTTGCCTATGGAACCATCTT CACTTTTATTGTGAGTTGTGCTAATTTAACAACATTTTTTTTAAAATTTGAACCAGCATATCTGTTATGGCTTAACATTC TTCTTGGACAGGGAATGGTTATTCTCTGGCTTACATCAATGATTCCGCAACTCACAACTTTGGCATGTTCTCATTTTCAA CATGTTTGTAATGGACCAACAGCATTCCAGCTTGCTGTTCTGTTTACATCTTTTGTGTTTACGTCTATCGGAGCTGGTTT TGTTAGACCTTGTTCTATAATGTTTGGTGCTGATCAATTGGAACAAAAAGGCAACCCTGAAAACAAGAAGATTGTTGAGA GCTATTTCAATTGGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1441 ACCAATTGAAATAGCTCTCAACAATCTTCTTGTTTTCAGGGTTGCCTTTT 1490 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ACCAATTGAAATAGCTCTCAACAATCTTCTTGTTTTCAGGGTTGCCTTTT 50 Wildtype 1491 TGTTCCAATTGATCAGCACCAAACATTATAGAACAAGGTCTAACAAAACC 1540 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TGTTCCAATTGATCAGCACCAAACATTATAGAACAAGGTCTAACAAAACC 100 Wildtype 1541 AGCTCCGATAGACGTAAACACAAAAGATGTAAACAGAACAGCAAGCTGGA 1590 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AGCTCCGATAGACGTAAACACAAAAGATGTAAACAGAACAGCAAGCTGGA 150 Wildtype 1591 ATGCTGTTGGTCCATTACAAACATGTTGAAAATGAGAACATGCCAAAGTT 1640 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 ATGCTGTTGGTCCATTACAAACATGTTGAAAATGAGAACATGCCAAAGTT 200 Wildtype 1641 GTGAGTTGCGGAATCATTGATGTAAGCCAGAGAATAACCATTCCCTGTCC 1690 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GTGAGTTGCGGAATCATTGATGTAAGCCAGAGAATAACCATTCCCTGTCC 250 Wildtype 1691 AAGAAGAATGTTAAGCCATAACAGATATGCTGGTTCAAATTTTAAAAAAA 1740 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 AAGAAGAATGTTAAGCCATAACAGATATGCTGGTTCAAATTTTAAAAAAA 300 Wildtype 1741 ATGTTGTTAAATTAGCACAACTCACAATAAAAGTGAAGATGGTTCCATAG 1790 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 ATGTTGTTAAATTAGCACAACTCACAATAAAAGTGAAGATGGTTCCATAG 350 Wildtype 1791 GCAACAGCATTATATCGACCCCAATAACAATCAGCAATAATCGCCCCAAC 1840 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GCAACAGCATTATATCGACCCCAATAACAATCAGCAATAATCGCCCCAAC 400 Wildtype 1841 AATAGCCAATCCACTTGAAAGTGCACCCCATATACCAATAATACTAGTGC 1890 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\| MutationRev 401 AATAGCCAATCCACTTGAAAGTGCACCCCATATACC---AATACTAGTGC 447 gRNA ATAATACTAGTGC Wildtype 1891 CAGTAGCAGCACTCATGTTATAATATGTCATCAAATAAATTATCATGTTT 1940 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 448 CAGTAGCAGCACTCATGTTATAATATGTCATCAAATAAATTATCATGTTT 497 gRNA ATAATACTAGTGCCAGTAGC Wildtype 1941 GTTTGTGAACCATAACTTGCTATCCTTTCAAATGATTCATTCACTGAAAA 1990 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 498 GTTTGTGAACCATAACTTGCTATCCTTTCAAATGATTCATTCACTGAAAA 547 Wildtype 1991 CAATAGCAACAACAATAACAAAAAATTGATATTAGAATTAACACATTGCA 2040 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 548 CAATAGCAACAACAATAACAAAAAATTGATATTAGAATTAACACATTGCA 597 Wildtype 2041 AAACAATTTGAAGTAAAAGAGTAGAGTGAGAGTAGTACAAATGATGAATG 2090 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 598 AAACAATTTGAAGTAAAAGAGTAGAGTGAGAGTAGTACAAATGATGAATG 647 Wildtype 2091 GCATGGTC 2098 \|\|\|\|\|\|\|\| MutationRev 648 GCATGGTC 655
CRT0138-6-11 (T1 )	CRT0138	CTG0138-18	CGR0138-18	gene : Solyc05g009500 (Peptide Transporter 3)	-64 bp/-64 bp Is tDNA present: No Mutation sequence: CTTTGACTATTGAGTGAGTTAACTAAGACGTTTATTACGTGACCATCTCATTTAAACGTTTAATTTTCAGATTGTTGGAC AGAGCAGCAATAGTAACAGAGGAGGACAACCCTAAATCTCCGAATCTATGGAGGCTAAACACAGTTCATCGCGTAGAAGA ACTCAACAATCCACATTCTCAGTCCAACAAGCCAAAACAATGAACAGACACCTACTAAACTCCTTCGAAATCCCAGCTGC CTCGATGACTGTCTTCACATTAACGACAATGCTATGCACCATTGCCTTCTATGACCGCGTATTTGTACCTATTGCACGTA AGTTCACTGGACTCGAACGAGGAATCTCATTCCTAAGTAGAATGGGAATTGGATTCTTCATATCTGTTCTAGCTACATTA GTAGCTGGATTTATTGAAGTTAAACGAAAAAATGTTGCATCGACTTATGGACTAGTCGATAAACCTAAATCACTAGTTCC AATTTCAGTATTTTGGCTAGTGCCACAGTATTGTTTACATGGTATAGCTGAGGCATTTATGTCAATTGGACATCTTGAAT TTTTCTATGATCAAGCTCCAGAGAGTATGAGAAGTACAGCAACCGCGTTATTTTGGACATCGATTTCAGCTGGGAACTAT TTGAGTACATTTCTGGTTTCGTTAGTTCATAAATTTACCAATTGGCTGCCTGATAATAACTTGAATG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 389 CATTCAAGTTATTATCAGGCAGCCAATTGGTAAATTTATGAACTAACGAA 438 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 CATTCAAGTTATTATCAGGCAGCCAATTGGTAAATTTATGAACTAACGAA 50 Wildtype 439 ACCAGAAATGTACTCAAATAGTTCCCAGCTGAAATCGATGTCCAAAATAA 488 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 ACCAGAAATGTACTCAAATAGTTCCCAGCTGAAATCGATGTCCAAAATAA 100 Wildtype 489 CGCGGTTGCTGTACTTCTCATACTCTCTGGAGCTTGATCATAGAAAAATT 538 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CGCGGTTGCTGTACTTCTCATACTCTCTGGAGCTTGATCATAGAAAAATT 150 Wildtype 539 CAAGATGTCCAATTGACATAAATGCCTCAGCTATACCATGTAAACAATAC 588 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CAAGATGTCCAATTGACATAAATGCCTCAGCTATACCATGTAAACAATAC 200 Wildtype 589 TGTGGCACTAGCCAAAATACTGAAATTGGAACTAGTGATTTAGGTTTATC 638 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TGTGGCACTAGCCAAAATACTGAAATTGGAACTAGTGATTTAGGTTTATC 250 Wildtype 639 GACTAGTCCATAAGTCGATGCAACATTTTTTCGTTTAACTTCAATAAATC 688 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GACTAGTCCATAAGTCGATGCAACATTTTTTCGTTTAACTTCAATAAATC 300 Wildtype 689 CAGCTACTAATGTAGCTAGAACAGAAATGAAGAATCCAATTCCCATTCTA 738 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CAGCTACTAATGTAGCTAGAACAGATATGAAGAATCCAATTCCCATTCTA 350 Wildtype 739 CTTAGGAATGAGATTCCTCGTTCGAGTCCAGTGAACTTACGTGCAATAGG 788 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 CTTAGGAATGAGATTCCTCGTTCGAGTCCAGTGAACTTACGTGCAATAGG 400 Wildtype 789 TACAAATACGCGGTCATAGAAGGCAATGGTGCATAGCATTGTCGTTAATG 838 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 TACAAATACGCGGTCATAGAAGGCAATGGTGCATAGCATTGTCGTTAATG 450 Wildtype 839 TGAAGACAGTCATCGAGGCAGCTGGGATTTCGAAGGAGTTTAGTAGGTGT 888 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 TGAAGACAGTCATCGAGGCAGCTGGGATTTCGAAGGAGTTTAGTAGGTGT 500 Wildtype 889 CTGTTCATTGTTTTGGCTTGTTGGACTGAGAATGTGGATTGTTGAGCGTA 938 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 CTGTTCATTGTTTTGGCTTGTTGGACTGAGAATGTGGATTG--------- 541 gRNA GTTGAGCGTA Wildtype 939 TGCTGTGATCAGGATGATCCCTGATGCCCATATTGGACCCATTCTTATGA 988 MutationRev 541 -------------------------------------------------- 541 gRNA GTTGAGCGTATGCTGTGATC Wildtype 989 TCGATTTGAGTTCTTCTACGCGATGAACTGTGTTTAGCCTCCATAGATTC 1038 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 542 -----TTGAGTTCTTCTACGCGATGAACTGTGTTTAGCCTCCATAGATTC 586 Wildtype 1039 GGAGATTTAGGGTTGTCCTCCTCTGTTACTATTGCTGCTCTGTCCAACAA 1088 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 587 GGAGATTTAGGGTTGTCCTCCTCTGTTACTATTGCTGCTCTGTCCAACAA 636 Wildtype 1089 TCTGAAAATTAAACTTTTAAATGAGATGGTCACGTAATAAACGTCTTAGT 1138 \|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 637 TCTGAAAATTAAACGTTTAAATGAGATGGTCACGTAATAAACGTCTTAGT 686 Wildtype 1139 TAACTCACTCAATAGTCAAAG 1159 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 687 TAACTCACTCAATAGTCAAAG 707

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0138-6-11 (T1 )

CRT0138

CTG0138-16

CGR0138-16

gene : Solyc05g005950 (Solute Transporter 2)

-3 bp/-3 bp

Is tDNA present: No

Mutation sequence:

GACCATGCCATTCATCATTTGTACTACTCTCACTCTACTCTTTTACTTCAAATTGTTTTGCAATGTGTTAATTCTAATAT
CAATTTTTTGTTATTGTTGTTGCTATTGTTTTCAGTGAATGAATCATTTGAAAGGATAGCAAGTTATGGTTCACAAACAA
ACATGATAATTTATTTGATGACATATTATAACATGAGTGCTGCTACTGGCACTAGTATTGGTATATGGGGTGCACTTTCA
AGTGGATTGGCTATTGTTGGGGCGATTATTGCTGATTGTTATTGGGGTCGATATAATGCTGTTGCCTATGGAACCATCTT
CACTTTTATTGTGAGTTGTGCTAATTTAACAACATTTTTTTTAAAATTTGAACCAGCATATCTGTTATGGCTTAACATTC
TTCTTGGACAGGGAATGGTTATTCTCTGGCTTACATCAATGATTCCGCAACTCACAACTTTGGCATGTTCTCATTTTCAA
CATGTTTGTAATGGACCAACAGCATTCCAGCTTGCTGTTCTGTTTACATCTTTTGTGTTTACGTCTATCGGAGCTGGTTT
TGTTAGACCTTGTTCTATAATGTTTGGTGCTGATCAATTGGAACAAAAAGGCAACCCTGAAAACAAGAAGATTGTTGAGA
GCTATTTCAATTGGT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1441 ACCAATTGAAATAGCTCTCAACAATCTTCTTGTTTTCAGGGTTGCCTTTT   1490
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ACCAATTGAAATAGCTCTCAACAATCTTCTTGTTTTCAGGGTTGCCTTTT     50

Wildtype        1491 TGTTCCAATTGATCAGCACCAAACATTATAGAACAAGGTCTAACAAAACC   1540
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TGTTCCAATTGATCAGCACCAAACATTATAGAACAAGGTCTAACAAAACC    100

Wildtype        1541 AGCTCCGATAGACGTAAACACAAAAGATGTAAACAGAACAGCAAGCTGGA   1590
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AGCTCCGATAGACGTAAACACAAAAGATGTAAACAGAACAGCAAGCTGGA    150

Wildtype        1591 ATGCTGTTGGTCCATTACAAACATGTTGAAAATGAGAACATGCCAAAGTT   1640
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 ATGCTGTTGGTCCATTACAAACATGTTGAAAATGAGAACATGCCAAAGTT    200

Wildtype        1641 GTGAGTTGCGGAATCATTGATGTAAGCCAGAGAATAACCATTCCCTGTCC   1690
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GTGAGTTGCGGAATCATTGATGTAAGCCAGAGAATAACCATTCCCTGTCC    250

Wildtype        1691 AAGAAGAATGTTAAGCCATAACAGATATGCTGGTTCAAATTTTAAAAAAA   1740
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 AAGAAGAATGTTAAGCCATAACAGATATGCTGGTTCAAATTTTAAAAAAA    300

Wildtype        1741 ATGTTGTTAAATTAGCACAACTCACAATAAAAGTGAAGATGGTTCCATAG   1790
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 ATGTTGTTAAATTAGCACAACTCACAATAAAAGTGAAGATGGTTCCATAG    350

Wildtype        1791 GCAACAGCATTATATCGACCCCAATAACAATCAGCAATAATCGCCCCAAC   1840
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 GCAACAGCATTATATCGACCCCAATAACAATCAGCAATAATCGCCCCAAC    400

Wildtype        1841 AATAGCCAATCCACTTGAAAGTGCACCCCATATACCAATAATACTAGTGC   1890
                     ||||||||||||||||||||||||||||||||||||   |||||||||||
MutationRev      401 AATAGCCAATCCACTTGAAAGTGCACCCCATATACC---AATACTAGTGC    447
gRNA                                                      ATAATACTAGTGC

Wildtype        1891 CAGTAGCAGCACTCATGTTATAATATGTCATCAAATAAATTATCATGTTT   1940
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      448 CAGTAGCAGCACTCATGTTATAATATGTCATCAAATAAATTATCATGTTT    497
gRNA                 ATAATACTAGTGCCAGTAGC

Wildtype        1941 GTTTGTGAACCATAACTTGCTATCCTTTCAAATGATTCATTCACTGAAAA   1990
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      498 GTTTGTGAACCATAACTTGCTATCCTTTCAAATGATTCATTCACTGAAAA    547

Wildtype        1991 CAATAGCAACAACAATAACAAAAAATTGATATTAGAATTAACACATTGCA   2040
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      548 CAATAGCAACAACAATAACAAAAAATTGATATTAGAATTAACACATTGCA    597

Wildtype        2041 AAACAATTTGAAGTAAAAGAGTAGAGTGAGAGTAGTACAAATGATGAATG   2090
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      598 AAACAATTTGAAGTAAAAGAGTAGAGTGAGAGTAGTACAAATGATGAATG    647

Wildtype        2091 GCATGGTC   2098
                     ||||||||
MutationRev      648 GCATGGTC    655

CRT0138-6-11 (T1 )

CRT0138

CTG0138-18

CGR0138-18

gene : Solyc05g009500 (Peptide Transporter 3)

-64 bp/-64 bp

Is tDNA present: No

Mutation sequence:

CTTTGACTATTGAGTGAGTTAACTAAGACGTTTATTACGTGACCATCTCATTTAAACGTTTAATTTTCAGATTGTTGGAC
AGAGCAGCAATAGTAACAGAGGAGGACAACCCTAAATCTCCGAATCTATGGAGGCTAAACACAGTTCATCGCGTAGAAGA
ACTCAACAATCCACATTCTCAGTCCAACAAGCCAAAACAATGAACAGACACCTACTAAACTCCTTCGAAATCCCAGCTGC
CTCGATGACTGTCTTCACATTAACGACAATGCTATGCACCATTGCCTTCTATGACCGCGTATTTGTACCTATTGCACGTA
AGTTCACTGGACTCGAACGAGGAATCTCATTCCTAAGTAGAATGGGAATTGGATTCTTCATATCTGTTCTAGCTACATTA
GTAGCTGGATTTATTGAAGTTAAACGAAAAAATGTTGCATCGACTTATGGACTAGTCGATAAACCTAAATCACTAGTTCC
AATTTCAGTATTTTGGCTAGTGCCACAGTATTGTTTACATGGTATAGCTGAGGCATTTATGTCAATTGGACATCTTGAAT
TTTTCTATGATCAAGCTCCAGAGAGTATGAGAAGTACAGCAACCGCGTTATTTTGGACATCGATTTCAGCTGGGAACTAT
TTGAGTACATTTCTGGTTTCGTTAGTTCATAAATTTACCAATTGGCTGCCTGATAATAACTTGAATG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         389 CATTCAAGTTATTATCAGGCAGCCAATTGGTAAATTTATGAACTAACGAA    438
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 CATTCAAGTTATTATCAGGCAGCCAATTGGTAAATTTATGAACTAACGAA     50

Wildtype         439 ACCAGAAATGTACTCAAATAGTTCCCAGCTGAAATCGATGTCCAAAATAA    488
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 ACCAGAAATGTACTCAAATAGTTCCCAGCTGAAATCGATGTCCAAAATAA    100

Wildtype         489 CGCGGTTGCTGTACTTCTCATACTCTCTGGAGCTTGATCATAGAAAAATT    538
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 CGCGGTTGCTGTACTTCTCATACTCTCTGGAGCTTGATCATAGAAAAATT    150

Wildtype         539 CAAGATGTCCAATTGACATAAATGCCTCAGCTATACCATGTAAACAATAC    588
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 CAAGATGTCCAATTGACATAAATGCCTCAGCTATACCATGTAAACAATAC    200

Wildtype         589 TGTGGCACTAGCCAAAATACTGAAATTGGAACTAGTGATTTAGGTTTATC    638
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TGTGGCACTAGCCAAAATACTGAAATTGGAACTAGTGATTTAGGTTTATC    250

Wildtype         639 GACTAGTCCATAAGTCGATGCAACATTTTTTCGTTTAACTTCAATAAATC    688
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 GACTAGTCCATAAGTCGATGCAACATTTTTTCGTTTAACTTCAATAAATC    300

Wildtype         689 CAGCTACTAATGTAGCTAGAACAGAAATGAAGAATCCAATTCCCATTCTA    738
                     |||||||||||||||||||||||||.||||||||||||||||||||||||
MutationRev      301 CAGCTACTAATGTAGCTAGAACAGATATGAAGAATCCAATTCCCATTCTA    350

Wildtype         739 CTTAGGAATGAGATTCCTCGTTCGAGTCCAGTGAACTTACGTGCAATAGG    788
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 CTTAGGAATGAGATTCCTCGTTCGAGTCCAGTGAACTTACGTGCAATAGG    400

Wildtype         789 TACAAATACGCGGTCATAGAAGGCAATGGTGCATAGCATTGTCGTTAATG    838
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 TACAAATACGCGGTCATAGAAGGCAATGGTGCATAGCATTGTCGTTAATG    450

Wildtype         839 TGAAGACAGTCATCGAGGCAGCTGGGATTTCGAAGGAGTTTAGTAGGTGT    888
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 TGAAGACAGTCATCGAGGCAGCTGGGATTTCGAAGGAGTTTAGTAGGTGT    500

Wildtype         889 CTGTTCATTGTTTTGGCTTGTTGGACTGAGAATGTGGATTGTTGAGCGTA    938
                     |||||||||||||||||||||||||||||||||||||||||         
MutationRev      501 CTGTTCATTGTTTTGGCTTGTTGGACTGAGAATGTGGATTG---------    541
gRNA                                                         GTTGAGCGTA

Wildtype         939 TGCTGTGATCAGGATGATCCCTGATGCCCATATTGGACCCATTCTTATGA    988
                                                                       
MutationRev      541 --------------------------------------------------    541
gRNA                 GTTGAGCGTATGCTGTGATC

Wildtype         989 TCGATTTGAGTTCTTCTACGCGATGAACTGTGTTTAGCCTCCATAGATTC   1038
                          |||||||||||||||||||||||||||||||||||||||||||||
MutationRev      542 -----TTGAGTTCTTCTACGCGATGAACTGTGTTTAGCCTCCATAGATTC    586

Wildtype        1039 GGAGATTTAGGGTTGTCCTCCTCTGTTACTATTGCTGCTCTGTCCAACAA   1088
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      587 GGAGATTTAGGGTTGTCCTCCTCTGTTACTATTGCTGCTCTGTCCAACAA    636

Wildtype        1089 TCTGAAAATTAAACTTTTAAATGAGATGGTCACGTAATAAACGTCTTAGT   1138
                     ||||||||||||||.|||||||||||||||||||||||||||||||||||
MutationRev      637 TCTGAAAATTAAACGTTTAAATGAGATGGTCACGTAATAAACGTCTTAGT    686

Wildtype        1139 TAACTCACTCAATAGTCAAAG   1159
                     |||||||||||||||||||||
MutationRev      687 TAACTCACTCAATAGTCAAAG    707