Plant Crispr Database

Plant Information

Name: CRT0082-2-4-7

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0082-2-4

Transformation: CRT0082
transformation CRT0082 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0082-2-4-7 (T2 )	CRT0082	CTG0082-1	CGR0082-1	gene : Solyc02g065110 (MKKK15)	+1 bp (A/T) and 1 bp substitution Is tDNA present: Yes Mutation sequence: GGGTGATTTTTTTAACTCCATATTTGAATTTGTGGCTGTGATACATCAAGTTCATGCAATGCATTGGTGGCCGAGCTTCT CATCTTCCACCCGTGCCTCTACCTCCGAAAGAAAAGTTCATTCCGATGATAGCGTGATCGTCACGCGCGGGGGCGTTCGT TCGGCCCTGAAATCAGCTCTCTTCGGCACCCGTAGCCGTATGCGCCAAATATCCCGGAAGAAGAAGAGGCCCCAACACCG TGGTGGTGACCACCATAGTGTCGANCGTCGGAGATGATTGGCACTCTCAGTATTCGTATGAAAGTTCTCTACAGGAGGAA CGTCCGCCGCCGCAACCCCTCCCTTTGCCGGAATTACATATGATGCTCCGTCAACATCCTAATTTGGTTCAAGCCCCCAG CGTCCCACTTCCTTCGCCTAGTGAAGCAATTTCGCATCATAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4832 CGAAATTGCTTCACTAGGCGAAGGAAGTGGGACGCTGGGGGCTTGAACCA 4881 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 CGAAATTGCTTCACTAGGCGAAGGAAGTGGGACGCTGGGGGCTTGAACCA 50 Wildtype 4882 AATTAGGATGTTGACGGAGCATCATATGTAATTCCGGCAAAGGGAGGGGT 4931 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 AATTAGGATGTTGACGGAGCATCATATGTAATTCCGGCAAAGGGAGGGGT 100 Wildtype 4932 TGCGGCGGCGGACGTTCCTCCTGTAGAGAACTTTCATACGAATACTGAGA 4981 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TGCGGCGGCGGACGTTCCTCCTGTAGAGAACTTTCATACGAATACTGAGA 150 Wildtype 4982 GTGCCAATCATCTCCGACG-TCGACACTATGGTGGTCACCACCACGGTGT 5030 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GTGCCAATCATCTCCGACGCTCGACACTATGGTGGTCACCACCACGGTGT 200 gRNA ACG-TCGACACTATGGTGGTC Wildtype 5031 TGGGGCCTCTTCTTCTTCCGGGATATTTGGCGCATACGGCTACGGGTGCC 5080 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TGGGGCCTCTTCTTCTTCCGGGATATTTGGCGCATACGGCTACGGGTGCC 250 Wildtype 5081 GAAGAGAGCTGATTTCAGGGCCGAACGAACGCCCCCGCGCGTGACGATCA 5130 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GAAGAGAGCTGATTTCAGGGCCGAACGAACGCCCCCGCGCGTGACGATCA 300 Wildtype 5131 CGCTATCATCGGAATGAACTTTTCTTTCGGAGGTAGAGGCACGGGTGGAA 5180 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CGCTATCATCGGAATGAACTTTTCTTTCGGAGGTAGAGGCACGGGTGGAA 350 Wildtype 5181 GATGAGAAGCTCGGCCACCAATGCATTGCATGAA 5214 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GATGAGAAGCTCGGCCACCAATGCATTGCATGAA 384

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0082-2-4-7 (T2 )

CRT0082

CTG0082-1

CGR0082-1

gene : Solyc02g065110 (MKKK15)

+1 bp (A/T) and 1 bp substitution

Is tDNA present: Yes

Mutation sequence:

GGGTGATTTTTTTAACTCCATATTTGAATTTGTGGCTGTGATACATCAAGTTCATGCAATGCATTGGTGGCCGAGCTTCT
CATCTTCCACCCGTGCCTCTACCTCCGAAAGAAAAGTTCATTCCGATGATAGCGTGATCGTCACGCGCGGGGGCGTTCGT
TCGGCCCTGAAATCAGCTCTCTTCGGCACCCGTAGCCGTATGCGCCAAATATCCCGGAAGAAGAAGAGGCCCCAACACCG
TGGTGGTGACCACCATAGTGTCGANCGTCGGAGATGATTGGCACTCTCAGTATTCGTATGAAAGTTCTCTACAGGAGGAA
CGTCCGCCGCCGCAACCCCTCCCTTTGCCGGAATTACATATGATGCTCCGTCAACATCCTAATTTGGTTCAAGCCCCCAG
CGTCCCACTTCCTTCGCCTAGTGAAGCAATTTCGCATCATAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4832 CGAAATTGCTTCACTAGGCGAAGGAAGTGGGACGCTGGGGGCTTGAACCA   4881
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 CGAAATTGCTTCACTAGGCGAAGGAAGTGGGACGCTGGGGGCTTGAACCA     50

Wildtype        4882 AATTAGGATGTTGACGGAGCATCATATGTAATTCCGGCAAAGGGAGGGGT   4931
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 AATTAGGATGTTGACGGAGCATCATATGTAATTCCGGCAAAGGGAGGGGT    100

Wildtype        4932 TGCGGCGGCGGACGTTCCTCCTGTAGAGAACTTTCATACGAATACTGAGA   4981
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 TGCGGCGGCGGACGTTCCTCCTGTAGAGAACTTTCATACGAATACTGAGA    150

Wildtype        4982 GTGCCAATCATCTCCGACG-TCGACACTATGGTGGTCACCACCACGGTGT   5030
                     ||||||||||||||||||| ||||||||||||||||||||||||||||||
MutationRev      151 GTGCCAATCATCTCCGACGCTCGACACTATGGTGGTCACCACCACGGTGT    200
gRNA                                 ACG-TCGACACTATGGTGGTC

Wildtype        5031 TGGGGCCTCTTCTTCTTCCGGGATATTTGGCGCATACGGCTACGGGTGCC   5080
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TGGGGCCTCTTCTTCTTCCGGGATATTTGGCGCATACGGCTACGGGTGCC    250

Wildtype        5081 GAAGAGAGCTGATTTCAGGGCCGAACGAACGCCCCCGCGCGTGACGATCA   5130
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 GAAGAGAGCTGATTTCAGGGCCGAACGAACGCCCCCGCGCGTGACGATCA    300

Wildtype        5131 CGCTATCATCGGAATGAACTTTTCTTTCGGAGGTAGAGGCACGGGTGGAA   5180
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 CGCTATCATCGGAATGAACTTTTCTTTCGGAGGTAGAGGCACGGGTGGAA    350

Wildtype        5181 GATGAGAAGCTCGGCCACCAATGCATTGCATGAA   5214
                     ||||||||||||||||||||||||||||||||||
MutationRev      351 GATGAGAAGCTCGGCCACCAATGCATTGCATGAA    384