Name: Solyc02g065110
(Exon Intron)
Length: 5214
CATGGGCATGTAATCATAGTAGTCGGCCCAGAATGCCTCTGTTCTTCAATGGGATGAACGGGGGATGACCATGGGAGTTGAATGAGCAAAATAACAGAGTCCCGTTGCTATGTTAGAATATGAATGATGTCATTGCTGCTGTGTAGGCATAAAGCTTTGAGGCAGGCAGACATTTAAGTGAACTTGCTCATTTGAGGACT
CCATTCTTTAGATCACAATTTGTTCTTCTTTGCCAGATCTTTCCGAGTGTTATCTTTCTAATGATACATGCACCGCCTCAAGTTTATAACTTAGCTGCTC
TCTTTGAGAATGCAACTTTTCCTGTCAAAGGTATACTATGGGATGAAGAAACACAACCAGCAAAAGAGGAAAGGGAATAAGGGACTGAATATACGTACTG
TTAGTCTTATCCCGTCAACTTGCTGGACAAAAGAAGGAACTTCTGGCTGATGCGACATTCTAACAAATGGATGTTCTAGCAACATGCTAGCTGATGGCCT
CTCTGCTGGATTTCTACGGAAACAACAATGCAAAAAATCTTTCCCTTCTAGTGATAATGTTTCAGGTATTGGTGGGGTATCTTTTAGAACCTTGAACATC
GCTGCAGCCTGTCATAGTTGAATTATTTGATTTGTAAGTTTTAAGAGTTTATAGAAAGCACTACATGGTCTTGCTCTTAGGTGAAATTATACAAAAAAAA
GCTTGCACAAGTTGAGTATAAACTATACTCAAGTAGCTATGATTTTTCAGCTGCTTAACATTCCTCCATTTCTGATGTTAAAAAGTTCATAAAACAATTA
AATGATTTGATAATTCATAATGACCAAAGGCAAATCTAGACATAGTGAAAGATATCCTTAACTCTGTTAGATGGCCAATGACAAAAATAAAGGTACATTG
GGAGAAATCAGATATTTGATTACACTAATGCCCTGGTCTAATGGAATACCAGCTGCACAGCTAGAGTAACAAATTCATATGACCCCTTGAAACAAGAAAA
TGTAGCATAATTAGTTATTATTTGCTAAGTTCGTGGAGCCATGTGAATCTAAGAGGGCATGTCAAGAAATGGATTTCTGCCAGGAAAGATCACGTACGAG
CTAAAAAATGCAGAAATAAAGTCCTTACAGCTTCATATTCACTCCAAGGAGGTTTGCCATTCAGCATCTCAATTATAGTACAACCCAAACTCCATATATC
AATAGCAAAGGCAAGATCGCTACTATTATCTCTCTGCATAACAGAGTGCATAAGCTGCAGGAAGCAGAAAACATAAGCTGTAGGAGGTGAGAGAACATAA
TTAGAAAGATATGCTGTTGAAGGGAAGTACCTCAGGAGCCATCCAATATGGACTTCCTTTTAAAGAGAGATTAGCTGAATGCCCGTTTAGCTGCATAACA
ACACTTGAGGTTCAAAGAAAAATATAACGCTAGCAAAAGTCTGGCAACAGCCAGTCTGTTAGTCTAAGATCTGTTGATTATACATCTTAGAGTGACAACA
TAGAAAAATTGTTATAACTATTATTGTTGTTTTTCTGAATATACATTCTTGTTTTACATAGCAGCACAAGAGAATGTCGTCTCCAAATTAAAACATTCAC
AGCTATTATTACAAAAAAAGAAGATGTTTAAAACACCCATATTTCTCCACAATGCTATAGATGAAGCATCTAAAAACGCATATTAACACTCCACTATGAA
GAGATTTACCATGTAAAGCACAAAGATCTCTGGATCAATCATTACATTCTACTAAATAGAACATTCTTTACTTAAAAAAAACTGCAAACTGAAAAAATGA
CTTGAGATAGAGTAACGTACGTGCTTAGCCATCCCAAAGTCTGCAAGTTTGACAACCCCATAAGCATCAACAAGCAAATTAGCCCCTTTAATGTCCCTGT
ACAAGGAACATTTCAAAATGGTTCTTCAGAAGGTTCTATATATTATATCTATCATAGTTGAATATCTGACTATCACCTGTGAATGGTCTTTTTACTATGC
AAGTACGCTAACCCGCAAAGAATATGGCGAGTGAAATTCCTCACAATAGATTCTGTAATTTCCCCACAATGGTCTTGGATAAATTTATTGATAGAACCAG
GATGAACATATTCTAGATAAATGTAAAAGCGGTCATCAACCTGCAATATCAAAGAATAAAACACAATCACTGAACAGTATGTAGAAGGATGAATTAAGTT
TATAACTTTGTAAAAATTTCTTACTATTTCACTGCCATAATACTGGACAATGTTTGGGTGCTTGAGGTGGCTGAGAACATTGATTTCCTGGACCAACATA
AAGAATAAATCATGACCATTATGCTCAATAAATGGTGCTTCTGATTCTTAGCATGCTTTCAATTCATCAGTAAAGAGGCAGACAGTTGAGATTTTGAACA
TTCTAAACCATAGTGATTATGTTAACCAATCAAGCAAGTTGTATATCTATATATCTACCAAAAAACATGGATATGTAAAACTAATATCCTGAGAAAGTCA
GATAAACGATTTTCTGTTGAAGGTTAAAGTGAAAAGGTATGGAATCTCCACTCATTAATTTGTAACTTAAAGATGTTAATCCTCAACATAGTTTTCAGTA
AGTTAAGACTGTCTGTATGTTCTATTTTACCGTACAAAGCCTTCATTTTTTCCATTAATTGCACCCTTTCAGTTCCTTTATTGGCCAAACTTTTTTGTAA
TTGAAATATCACAATAACATGCACAAACTTTTCATGTACTTAATTTCTCCCTTTATAAATTGGATAAAAAATATTAATCCACCACACTTTGTTTTCATCA
TCTAATAGTTCTTCTGTAAAGAGTATTTTTTATTGGTCACTATTTTTCTTATTGATTTGTGCCTTCGTTCATTTTTTATTTTCATGGTTGTTGGAGAAAC
ACATAATCTAATAGTTCTTGTAATATTTTTTTATTGGTCACTATTTTTCTTATTGGTTTGTGTCCTTCATTCATTTTTTTATTTTTAATGATTGTTGGAG
TATTTATATCCCAAGAAATTTCAGCCTTGGGGCTTAGTTCTAGCCTTACAAGTTGCTCATCATTCACCAATATTTAAGTTATAGGCAAAAAGTAAACACT
AAAAAAAGTTTATTTAATATGCACAAAAAGACATATAGGAGTACGTCAGACAATGTCGAGCAATTTTATATCTTGGGCCTATATAAATTATAACAATAAT
AGAGGTCAAACGTGCAAGGCACGTTTCCAAAAATAGTAATGCAGATAAACCCCAAACTCCAAATATTTCGGCCAATTTTAGTAATGCAGGAGTGCCCATA
TATGATTTTTATTAACTCCAATAAAAATCTATACAGAGAATCCAAGTATTCAAGGATTATGAAACAAAGACAGTGAAGGAAATCTGACCTGTTCTAGCTG
CCTTATAGACTCAGCAGATTTTGGGTCATCTGGTAATAATTCAACTTCTTTCAATGCAGACAAAGCTCCTGTCTCTCTGGTTAAGATCATAGTGTTACAT
TAGAAAATATTGAAGGACTTAAGATATATTTATACTATCAGCATGTTACAGCATCATACAGACATGCAACAACATCCATCAACAAGATCTATATACCTGT
TTGATGCTACGTAAACACTTCCAAATGTCCCACGTCCAATAAGCTTTCCTTTTTTCCACTGGCCTTTAATAGGTGATATATCTGCTTTAGGGGTAGGTGG
TGAAACGGGATGGGTAGCTAGAGGAGGAAGGGGTAAAGGATGGACATTTCCTTGAGCGCAATTCTCACGACGCGTCATTGGGCACTGATCATTTGGTAGT
GGATGATGTAAAGGTGAAATAGATCCACTTGGACTCCTGGATCTTCTCTGAGGACTTAATCTTGGACTGTGAAGAGGAGAGTTATCAACACTAAAAGCAT
GGTTATAAGAAAAGCCTAAACCATGAGGGGCTTCTGAAGGAGGCATCTCAGGGGCAGACCAAACTTGAAATACACTAGGAGGTGTGACGAAAAGAGGACT
CGTTATATGATCACAACTTTTCAGTTGTCTATGTGTAGGACTTGCATAAGGACTAGTTGGAGCACTAGTCCAAAAGTCAGGAGGGATTCTTTCTGGGACA
AGCAGCATCCGAGGTGGCATCATCCTGGACAATGGAGTTGCAGAGTGATCTGTTCTCTTTCGAGACTCTTGGTTTAATAGTCTGCTGAAATATCAAACAA
AGAACAAAATCATCAGGTCTACATAATCTGAAACCTACAATATATGACTACTACAACAACATACCTAGTTTAACAAGTGAGGTCCGGGCAGGGTAAAGTG
TATGCAGACCTTACCCCTACCTTGTGCAAGCAGTGAGGTTGTTTCAGATAGACAATCGACTCAAGGAAAACATAAACCCAACATTTATGGAAACATAAGC
AGGACAGTAAGAAACCCGCAGCATAAGAGACAACTAGTAGGTCAATACAATAAATGACTACATGAGTGTTAATTGCCAGCTACGCCTAGAGAAAAAGAAA
ATTGTTATTGTGTTGATTTCAAATTTGACAAATTCAGTCACTAAAAATATCAGGAAATTGTTCAAAACATGATGACCCACAACCAACTAAAACCCTAAGA
ATTTGATTGTAAAATGCACAAATTAATTCATTTGAATAAGAAATTTAGAGACCTAATAATTCGAAGAAAGAAGAGTTTCCGATCGAATTTACACAAATAT
AATTTAAACTTGAGCAAAATAAGAAACAATTACTGGACATATTGAGAAATTTTCACATACCCCTCTCGTGTTGCTCCACCATTGAAAGCATCCATCCTTT
CTCTTTCTATTTCCTCTCCAGCTTTATGATGCGAAATTGCTTCACTAGGCGAAGGAAGTGGGACGCTGGGGGCTTGAACCAAATTAGGATGTTGACGGAG
CATCATATGTAATTCCGGCAAAGGGAGGGGTTGCGGCGGCGGACGTTCCTCCTGTAGAGAACTTTCATACGAATACTGAGAGTGCCAATCATCTCCGACG
TCGACACTATGGTGGTCACCACCACGGTGTTGGGGCCTCTTCTTCTTCCGGGATATTTGGCGCATACGGCTACGGGTGCCGAAGAGAGCTGATTTCAGGG
CCGAACGAACGCCCCCGCGCGTGACGATCACGCTATCATCGGAATGAACTTTTCTTTCGGAGGTAGAGGCACGGGTGGAAGATGAGAAGCTCGGCCACCA
ATGCATTGCATGAA
The mutation and phenotype for selected feature: Solyc02g065110
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-1 (T0 ) | +1 bp (62.7%); WT (31.3%) | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-1-5 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TAATAGGGTGATTTTTTTAACTCCATATTTGAATTTGTGGCTGTGATACATCAAGTTCATGCAATGCATTGGTGGCCGAG CTTCTCATCTTCCACCCGTGCCTCTACCTCCGAAAGAAAAGTTCATTCCGATGATAGCGTGATCGTCACGCGCGGGGGCG TTCGTTCGGCCCTGAAATCAGCTCTCTTCGGCACCCGTAGCCGTATGCGCCAAATATCCCGGAAGAAGAAGAGGCCCCAA CACCGTGGTGGTGACCACCATAGTGTCGAGCGTCGGAGATGATTGGCACTCTCAGTATTCGTATGAAAGTTCTCTACAGG AGGAACGTCCGCCGCCGCAACCCCTCCCTTTGCCGGAATTACATATGATGCTCCGTCAACATCCTAATTTGGTTCAAGCC CCCAGCGTCCCACTTCCTTCGCCTAGTGAAGCAATTTCG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-2 (T0 ) | -1 bp (10.4%); WT (85%); +1 bp (2.1%) | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-2-4 (T1 ) | +1 bp (72.6%); -5 bp (7%); -3/-2/-1; WT 6.3% | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-2-4-4 (T2 ) | WT 21%; +1 bp 75% | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-2-4-7 (T2 ) |
+1 bp (A/T) and 1 bp substitution
Is tDNA present: Yes
Mutation sequence: GGGTGATTTTTTTAACTCCATATTTGAATTTGTGGCTGTGATACATCAAGTTCATGCAATGCATTGGTGGCCGAGCTTCT CATCTTCCACCCGTGCCTCTACCTCCGAAAGAAAAGTTCATTCCGATGATAGCGTGATCGTCACGCGCGGGGGCGTTCGT TCGGCCCTGAAATCAGCTCTCTTCGGCACCCGTAGCCGTATGCGCCAAATATCCCGGAAGAAGAAGAGGCCCCAACACCG TGGTGGTGACCACCATAGTGTCGANCGTCGGAGATGATTGGCACTCTCAGTATTCGTATGAAAGTTCTCTACAGGAGGAA CGTCCGCCGCCGCAACCCCTCCCTTTGCCGGAATTACATATGATGCTCCGTCAACATCCTAATTTGGTTCAAGCCCCCAG CGTCCCACTTCCTTCGCCTAGTGAAGCAATTTCGCATCATAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-2-6 (T1 ) | azygous | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-2-7 (T1 ) | +1 bp (77.6%); -5 bp (2.7%); -3/-2/-1; WT 9.5% | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-3 (T0 ) | WT 47%; -5 (49.8%) | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-4 (T0 ) | WT 50.7%; -5 (46.7%) | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-4-1 (T1 ) | -5 bp (48.6%); WT 47% | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-4-1-1 (T2 ) | -5 bp/-5 bp | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-4-1-6 (T2 ) | -5 bp/-5 bp | |
| CRT0082 | RG-PtoR | CTG0082-1 | CGR0082-1 | CRT0082-5 (T0 ) | WT 67.3%; +1 (30%) |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
