Is tDNA present:

Mutation sequence:

ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAACTCTTGCCGTTCCAAAGATCCATCTTCTCT
TCCCCAAAATCCTAATTTCTTCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATTTCACCGGCTT
TCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGTCGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTG
AAAGAAGAGAAGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAAGAATCTGAAGAGGAGATTAG
TTTGGCCATAAAACCGCCTACAACTCCTCCACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA
AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGGATGAGCACGTCTTCAGTCTGAGGAGTACAG
TGGGATTTTAAGCGGTACTGATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACTGAAACTTTAG
TTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTCATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAG
ACCACTACAATTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTCAAGCATTCTAGACCTAGTTT
CTCATCTTCAAAAGGTACAATCTTTTTGTTTATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA
ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATTGAGTTCATTTTAAATAATTCTACTGTATCT
TTAATGAAATATATTCATATATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAATCTTTAATTTC
TTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGAAACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTT
CCCTCTATCTGTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTTTATGATAATTAAGACATTTG
CTTATCAACTCATCGCAAAGTTGCCAAGAAATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC
ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGTTCAAGAAGCTGATACCATGTAGCGTGGATG
GGAAAGTGAAGGAGAGTTTCGCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAATGTTGGAAATG
ATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAACAGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTA
TCATGGAGTAATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGCTAGTAATATTCATACAAGTA
GGAGGATGTCAACTTATGTCCACTCATCTACAGTATGCACAACCCTTTGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50

Wildtype          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100

Wildtype         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150

Wildtype         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200

Wildtype         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250
gRNA                                                        GAAAGAAGAGA

Wildtype         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300
gRNA                 GAAAGAAGAGAAGTTTCACG

Wildtype         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350

Wildtype         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400

Wildtype         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450

Wildtype         451 ATGAGCACGTCTTCAG-CTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    499
                     |||||||||||||||| |||||||||||||||||||||||||||||||||
Mutation         451 ATGAGCACGTCTTCAGTCTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    500

Wildtype         500 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    550

Wildtype         550 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    600

Wildtype         600 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    650

Wildtype         650 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    700

Wildtype         700 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    750

Wildtype         750 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    800

Wildtype         800 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    850

Wildtype         850 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    900

Wildtype         900 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    950

Wildtype         950 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA   1000

Wildtype        1000 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1050

Wildtype        1050 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1100

Wildtype        1100 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1150

Wildtype        1150 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1200

Wildtype        1200 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1250

Wildtype        1250 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1300

Wildtype        1300 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1350

Wildtype        1350 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1400

Wildtype        1400 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1450

Wildtype        1450 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1500

Wildtype        1500 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1550

Wildtype        1550 CAGTATGCACAACCCTTTGA   1569
                     ||||||||||||||||||||
Mutation        1551 CAGTATGCACAACCCTTTGA   1570

Is tDNA present:

Mutation sequence:

ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAACTCTTGCCGTTCCAAAGATCCATCTTCTCT
TCCCCAAAATCCTAATTTCTTCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATTTCACCGGCTT
TCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGTCGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTG
AAAGAAGAGAAGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAAGAATCTGAAGAGGAGATTAG
TTTGGCCATAAAACCGCCTACAACTCCTCCACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA
AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGGATGAGCACGTCTTCAGTCTGAGGAGTACAG
TGGGATTTTAAGCGGTACTGATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACTGAAACTTTAG
TTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTCATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAG
ACCACTACAATTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTCAAGCATTCTAGACCTAGTTT
CTCATCTTCAAAAGGTACAATCTTTTTGTTTATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA
ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATTGAGTTCATTTTAAATAATTCTACTGTATCT
TTAATGAAATATATTCATATATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAATCTTTAATTTC
TTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGAAACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTT
CCCTCTATCTGTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTTTATGATAATTAAGACATTTG
CTTATCAACTCATCGCAAAGTTGCCAAGAAATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC
ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGTTCAAGAAGCTGATACCATGTAGCGTGGATG
GGAAAGTGAAGGAGAGTTTCGCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAATGTTGGAAATG
ATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAACAGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTA
TCATGGAGTAATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGCTAGTAATATTCATACAAGTA
GGAGGATGTCAACTTATGTCCACTCATCTACAGTATGCACAACCCTTTGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50

Wildtype          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100

Wildtype         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150

Wildtype         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200

Wildtype         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250
gRNA                                                        GAAAGAAGAGA

Wildtype         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300
gRNA                 GAAAGAAGAGAAGTTTCACG

Wildtype         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350

Wildtype         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400

Wildtype         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450

Wildtype         451 ATGAGCACGTCTTCAG-CTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    499
                     |||||||||||||||| |||||||||||||||||||||||||||||||||
Mutation         451 ATGAGCACGTCTTCAGTCTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    500

Wildtype         500 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    550

Wildtype         550 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    600

Wildtype         600 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    650

Wildtype         650 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    700

Wildtype         700 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    750

Wildtype         750 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    800

Wildtype         800 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    850

Wildtype         850 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    900

Wildtype         900 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    950

Wildtype         950 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA   1000

Wildtype        1000 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1050

Wildtype        1050 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1100

Wildtype        1100 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1150

Wildtype        1150 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1200

Wildtype        1200 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1250

Wildtype        1250 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1300

Wildtype        1300 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1350

Wildtype        1350 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1400

Wildtype        1400 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1450

Wildtype        1450 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1500

Wildtype        1500 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1550

Wildtype        1550 CAGTATGCACAACCCTTTGA   1569
                     ||||||||||||||||||||
Mutation        1551 CAGTATGCACAACCCTTTGA   1570

Is tDNA present:

Mutation sequence:

ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAACTCTTGCCGTTCCAAAGATCCATCTTCTCT
TCCCCAAAATCCTAATTTCTTCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATTTCACCGGCTT
TCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGTCGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTG
AAAGAAGAGAAGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAAGAATCTGAAGAGGAGATTAG
TTTGGCCATAAAACCGCCTACAACTCCTCCACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA
AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGGATGAGCACGTCTTCAGTCTGAGGAGTACAG
TGGGATTTTAAGCGGTACTGATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACTGAAACTTTAG
TTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTCATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAG
ACCACTACAATTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTCAAGCATTCTAGACCTAGTTT
CTCATCTTCAAAAGGTACAATCTTTTTGTTTATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA
ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATTGAGTTCATTTTAAATAATTCTACTGTATCT
TTAATGAAATATATTCATATATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAATCTTTAATTTC
TTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGAAACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTT
CCCTCTATCTGTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTTTATGATAATTAAGACATTTG
CTTATCAACTCATCGCAAAGTTGCCAAGAAATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC
ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGTTCAAGAAGCTGATACCATGTAGCGTGGATG
GGAAAGTGAAGGAGAGTTTCGCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAATGTTGGAAATG
ATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAACAGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTA
TCATGGAGTAATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGCTAGTAATATTCATACAAGTA
GGAGGATGTCAACTTATGTCCACTCATCTACAGTATGCACAACCCTTTGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50

Wildtype          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100

Wildtype         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150

Wildtype         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200

Wildtype         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250

Wildtype         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300

Wildtype         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350

Wildtype         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400

Wildtype         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450

Wildtype         451 ATGAGCACGTCTTCAG-CTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    499
                     |||||||||||||||| |||||||||||||||||||||||||||||||||
Mutation         451 ATGAGCACGTCTTCAGTCTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    500

Wildtype         500 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    550

Wildtype         550 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    600

Wildtype         600 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    650

Wildtype         650 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    700

Wildtype         700 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    750

Wildtype         750 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    800

Wildtype         800 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    850

Wildtype         850 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    900

Wildtype         900 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    950

Wildtype         950 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA   1000

Wildtype        1000 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1050

Wildtype        1050 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1100

Wildtype        1100 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1150

Wildtype        1150 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1200

Wildtype        1200 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1250

Wildtype        1250 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1300

Wildtype        1300 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1350

Wildtype        1350 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1400

Wildtype        1400 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1450

Wildtype        1450 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1500

Wildtype        1500 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1550

Wildtype        1550 CAGTATGCACAACCCTTTGA   1569
                     ||||||||||||||||||||
Mutation        1551 CAGTATGCACAACCCTTTGA   1570

Is tDNA present:

Mutation sequence:

ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAACTCTTGCCGTTCCAAAGATCCATCTTCTCT
TCCCCAAAATCCTAATTTCTTCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATTTCACCGGCTT
TCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGTCGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTG
AAAGAAGAGAAGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAAGAATCTGAAGAGGAGATTAG
TTTGGCCATAAAACCGCCTACAACTCCTCCACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA
AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGGATGAGCACGTCTTCAGTCTGAGGAGTACAG
TGGGATTTTAAGCGGTACTGATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACTGAAACTTTAG
TTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTCATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAG
ACCACTACAATTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTCAAGCATTCTAGACCTAGTTT
CTCATCTTCAAAAGGTACAATCTTTTTGTTTATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA
ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATTGAGTTCATTTTAAATAATTCTACTGTATCT
TTAATGAAATATATTCATATATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAATCTTTAATTTC
TTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGAAACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTT
CCCTCTATCTGTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTTTATGATAATTAAGACATTTG
CTTATCAACTCATCGCAAAGTTGCCAAGAAATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC
ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGTTCAAGAAGCTGATACCATGTAGCGTGGATG
GGAAAGTGAAGGAGAGTTTCGCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAATGTTGGAAATG
ATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAACAGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTA
TCATGGAGTAATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGCTAGTAATATTCATACAAGTA
GGAGGATGTCAACTTATGTCCACTCATCTACAGTATGCACAACCCTTTGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAAAAGTTTGAAGCTTCGTTTCTCCAGAGTTATTGCTTCCTTCAA     50

Wildtype          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CTCTTGCCGTTCCAAAGATCCATCTTCTCTTCCCCAAAATCCTAATTTCT    100

Wildtype         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCCCATCACATACTAATGATAGTAATAATAAGCTCAGTACAAAGCTCATT    150

Wildtype         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCACCGGCTTTCCCTCTTAATAATCAAAATCAAAACCCTAGTACTAATGT    200

Wildtype         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CGCAGTCTCCGTTGGCTGTCGAAAGGAATTCAAGTGGCTGAAAGAAGAGA    250

Wildtype         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AGTTTCACGTGGTTTCCAGCTCTATCGTCTCTGAAGAAGAAGAAGAAGAA    300

Wildtype         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 GAATCTGAAGAGGAGATTAGTTTGGCCATAAAACCGCCTACAACTCCTCC    350

Wildtype         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 ACGATTCAGTAGTATATTTGTTGAGAAGAAGAAGACGACGAAGAAGAAGA    400

Wildtype         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGAAACAGAGAGTTAAAAAGACTAAAACAAAGAGTAGTAGGATGAGCAGG    450

Wildtype         451 ATGAGCACGTCTTCAG-CTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    499
                     |||||||||||||||| |||||||||||||||||||||||||||||||||
Mutation         451 ATGAGCACGTCTTCAGTCTGAGGAGTACAGTGGGATTTTAAGCGGTACTG    500

Wildtype         500 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATGATCAAACTTGGGATAATAATTATAAGGAAGAAGAAGAGGACGAAACT    550

Wildtype         550 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAACTTTAGTTTCATCTTCCAGAAGCTTTGATTTCTCAAGTGATGATTC    600

Wildtype         600 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 ATCTACTGATTTCAATCCTCAGTTGGAAACCATATGTGAGACCACTACAA    650

Wildtype         650 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTAGACGTCGGCGTCACAAGAGGAACAATGCCACCACCAAGAGGAGACTC    700

Wildtype         700 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAGCATTCTAGACCTAGTTTCTCATCTTCAAAAGGTACAATCTTTTTGTT    750

Wildtype         750 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TATGTGGCCAAAATCATCTTGAAGTTTGTAGTTTGCGATAATTTACAACA    800

Wildtype         800 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 ATATATTCCAGTGTAATTTAATAAGTGAGGTCGTATGCATAAACCATATT    850

Wildtype         850 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 GAGTTCATTTTAAATAATTCTACTGTATCTTTAATGAAATATATTCATAT    900

Wildtype         900 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 ATGATCATACAAATAACTGTTAATTGTATTAGATCAGACGAAAAAAAAAT    950

Wildtype         950 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 CTTTAATTTCTTAGACTCTTAGCTTAATCAAAATTCATTACACAAATTGA   1000

Wildtype        1000 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 AACGGAAGACTATTTAATTTATGTTATGTCTCAACATGTTCCCTCTATCT   1050

Wildtype        1050 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GTGTTATTTTCTTGTTGTGTTGGATATATGAAAATACATTCTAAATTTTT   1100

Wildtype        1100 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TATGATAATTAAGACATTTGCTTATCAACTCATCGCAAAGTTGCCAAGAA   1150

Wildtype        1150 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 ATATATATAAATTTGATTAATTATATTATGTGATGAATAGGTGGAAGATC   1200

Wildtype        1200 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 ATCAGTTTCTACGTCATCGGAGAGCGAGTTACCGGCGAGGTTATCGGTGT   1250

Wildtype        1250 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TCAAGAAGCTGATACCATGTAGCGTGGATGGGAAAGTGAAGGAGAGTTTC   1300

Wildtype        1300 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 GCGATAGTGAAGAAATCACAGGATCCATACGAGGATTTCAAGAGGTCAAT   1350

Wildtype        1350 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GTTGGAAATGATATTGGAGAAACAAATGTTTGACAAGAATGAGCTTGAAC   1400

Wildtype        1400 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 AGCTCTTACAATGTTTTCTGTCGTTGAACGGAAAGCATTATCATGGAGTA   1450

Wildtype        1450 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 ATAGTTGAGGCTTTCTCCAATATCTGGGAGACTTTGTTTTTAGGTAATGC   1500

Wildtype        1500 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 TAGTAATATTCATACAAGTAGGAGGATGTCAACTTATGTCCACTCATCTA   1550

Wildtype        1550 CAGTATGCACAACCCTTTGA   1569
                     ||||||||||||||||||||
Mutation        1551 CAGTATGCACAACCCTTTGA   1570