Is tDNA present:

Mutation sequence:

ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGAAGTTGTTGATTATTTCTATCCAAGTGTTAA
GATGTCTGTTATTTATAACTCCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCACCTCTAAACCT
AGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCACTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATA
TTGTTCCCTACTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAGCATTGAGATGATTCATGGTC
GTAGACATGTTTAAGATTTATCTTAGACCACAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT
AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTATTTTTATATGAATATTTGACCACTTTTTTC
TTTTCTTTTTTCTATATTAATTGACAGATCATGATAGATCCAGATGTCCTGGTCCTAGTGATCCATATCTCAGGGAACAT
CTACACTGGTATTGATATATGACTTTAAACTAACTCATTCAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTAC
TTTGGAAGGGAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAAAAAAAAAAAAAATCCCATGAG
TTAGTTTGGAGTTTTCTTTTTTTGTACCTTTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATGG
GAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAGACATGAATGCATCAGCAATTCATAGAGATAT
GCTTATATATGTAGGAGTAGTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTCGTGTTTTCGTT
TGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAAGTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATA
TTTTTAAAATGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAAAAAAAAAGGTAGAGTAGAAAT
TTTCACATCAAACGCCTCCTAAATATGTCCAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGCA
GAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTTTAAGTGACATATTTGTGCAATATAATTGATA
GTTGACAAATACCTTAAAATAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACAGATTGCTCTTT
TGGTATGTATTCTTAACCCATACTTGGAAATAATATTTTTTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGAT
GGTATTGATTCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAGAAATTATTCTTGGCTTTATTT
GACAAGTATATGAAATGTCTAGAAATGACAAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCTA
TATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATTGTATAACGTTTATGCATATAATTATGTATAA
CTAGGCCTCAATTTCAAACAATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATCTTTAGAAAACC
CAAATATAGGGAACGATTGTGTTCATCAATATTGGAGATTCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTG
TATCTTCAAATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTTATTTTACACTGTCGGTGCATA
CGTATAAATTAAATGTAATTGAAAAAATGTCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAATC
CACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATATTGAATGCACCAGTCTCCAGGGATCGATTCAG
TACTCGAAAATTCTCAGAAGAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAGGGAAACTGCTG
CCAGAAGGCGTTGAGATTGC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50

Wildtype          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100

Wildtype         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCC-TTAGTCAC    149
                     ||||||||||||||||||||||||||||||||||||||||| ||||||||
Mutation         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCAC    150
gRNA                                                       TCC-TTAGTCAC

Wildtype         150 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    200
gRNA                 TCCTTAGTCACCTCTAAACC

Wildtype         200 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    250

Wildtype         250 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    300

Wildtype         300 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    350

Wildtype         350 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    400

Wildtype         400 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    450

Wildtype         450 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    500

Wildtype         500 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    550

Wildtype         550 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    600

Wildtype         600 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    650

Wildtype         650 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    700

Wildtype         700 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    750

Wildtype         750 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    800

Wildtype         800 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    850

Wildtype         850 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    900

Wildtype         900 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    950

Wildtype         950 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA   1000

Wildtype        1000 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1050

Wildtype        1050 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1100

Wildtype        1100 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1150

Wildtype        1150 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1200

Wildtype        1200 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1250

Wildtype        1250 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1300

Wildtype        1300 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1350

Wildtype        1350 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1400

Wildtype        1400 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1450

Wildtype        1450 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1500

Wildtype        1500 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1550

Wildtype        1550 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1600

Wildtype        1600 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1650

Wildtype        1650 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1700

Wildtype        1700 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1701 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1750

Wildtype        1750 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1800

Wildtype        1800 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1850

Wildtype        1850 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1900

Wildtype        1900 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1950

Wildtype        1950 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   2000

Wildtype        2000 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2050

Wildtype        2050 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2100

Wildtype        2100 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2150

Wildtype        2150 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2180
                     |||||||||||||||||||||||||||||||
Mutation        2151 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2181

Is tDNA present:

Mutation sequence:

ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGAAGTTGTTGATTATTTCTATCCAAGTGTTAA
GATGTCTGTTATTTATAACTCCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCACCTCTAAACCT
AGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCACTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATA
TTGTTCCCTACTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAGCATTGAGATGATTCATGGTC
GTAGACATGTTTAAGATTTATCTTAGACCACAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT
AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTATTTTTATATGAATATTTGACCACTTTTTTC
TTTTCTTTTTTCTATATTAATTGACAGATCATGATAGATCCAGATGTCCTGGTCCTAGTGATCCATATCTCAGGGAACAT
CTACACTGGTATTGATATATGACTTTAAACTAACTCATTCAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTAC
TTTGGAAGGGAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAAAAAAAAAAAAAATCCCATGAG
TTAGTTTGGAGTTTTCTTTTTTTGTACCTTTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATGG
GAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAGACATGAATGCATCAGCAATTCATAGAGATAT
GCTTATATATGTAGGAGTAGTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTCGTGTTTTCGTT
TGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAAGTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATA
TTTTTAAAATGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAAAAAAAAAGGTAGAGTAGAAAT
TTTCACATCAAACGCCTCCTAAATATGTCCAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGCA
GAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTTTAAGTGACATATTTGTGCAATATAATTGATA
GTTGACAAATACCTTAAAATAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACAGATTGCTCTTT
TGGTATGTATTCTTAACCCATACTTGGAAATAATATTTTTTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGAT
GGTATTGATTCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAGAAATTATTCTTGGCTTTATTT
GACAAGTATATGAAATGTCTAGAAATGACAAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCTA
TATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATTGTATAACGTTTATGCATATAATTATGTATAA
CTAGGCCTCAATTTCAAACAATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATCTTTAGAAAACC
CAAATATAGGGAACGATTGTGTTCATCAATATTGGAGATTCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTG
TATCTTCAAATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTTATTTTACACTGTCGGTGCATA
CGTATAAATTAAATGTAATTGAAAAAATGTCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAATC
CACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATATTGAATGCACCAGTCTCCAGGGATCGATTCAG
TACTCGAAAATTCTCAGAAGAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAGGGAAACTGCTG
CCAGAAGGCGTTGAGATTGC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50

Wildtype          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100

Wildtype         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCC-TTAGTCAC    149
                     ||||||||||||||||||||||||||||||||||||||||| ||||||||
Mutation         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCAC    150
gRNA                                                       TCC-TTAGTCAC

Wildtype         150 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    200
gRNA                 TCCTTAGTCACCTCTAAACC

Wildtype         200 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    250

Wildtype         250 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    300

Wildtype         300 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    350

Wildtype         350 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    400

Wildtype         400 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    450

Wildtype         450 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    500

Wildtype         500 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    550

Wildtype         550 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    600

Wildtype         600 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    650

Wildtype         650 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    700

Wildtype         700 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    750

Wildtype         750 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    800

Wildtype         800 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    850

Wildtype         850 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    900

Wildtype         900 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    950

Wildtype         950 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA   1000

Wildtype        1000 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1050

Wildtype        1050 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1100

Wildtype        1100 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1150

Wildtype        1150 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1200

Wildtype        1200 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1250

Wildtype        1250 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1300

Wildtype        1300 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1350

Wildtype        1350 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1400

Wildtype        1400 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1450

Wildtype        1450 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1500

Wildtype        1500 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1550

Wildtype        1550 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1600

Wildtype        1600 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1650

Wildtype        1650 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1700

Wildtype        1700 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1701 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1750

Wildtype        1750 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1800

Wildtype        1800 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1850

Wildtype        1850 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1900

Wildtype        1900 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1950

Wildtype        1950 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   2000

Wildtype        2000 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2050

Wildtype        2050 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2100

Wildtype        2100 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2150

Wildtype        2150 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2180
                     |||||||||||||||||||||||||||||||
Mutation        2151 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2181

Is tDNA present:

Mutation sequence:

ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGAAGTTGTTGATTATTTCTATCCAAGTGTTAA
GATGTCTGTTATTTATAACTCCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCACCTCTAAACCT
AGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCACTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATA
TTGTTCCCTACTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAGCATTGAGATGATTCATGGTC
GTAGACATGTTTAAGATTTATCTTAGACCACAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT
AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTATTTTTATATGAATATTTGACCACTTTTTTC
TTTTCTTTTTTCTATATTAATTGACAGATCATGATAGATCCAGATGTCCTGGTCCTAGTGATCCATATCTCAGGGAACAT
CTACACTGGTATTGATATATGACTTTAAACTAACTCATTCAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTAC
TTTGGAAGGGAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAAAAAAAAAAAAAATCCCATGAG
TTAGTTTGGAGTTTTCTTTTTTTGTACCTTTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATGG
GAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAGACATGAATGCATCAGCAATTCATAGAGATAT
GCTTATATATGTAGGAGTAGTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTCGTGTTTTCGTT
TGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAAGTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATA
TTTTTAAAATGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAAAAAAAAAGGTAGAGTAGAAAT
TTTCACATCAAACGCCTCCTAAATATGTCCAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGCA
GAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTTTAAGTGACATATTTGTGCAATATAATTGATA
GTTGACAAATACCTTAAAATAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACAGATTGCTCTTT
TGGTATGTATTCTTAACCCATACTTGGAAATAATATTTTTTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGAT
GGTATTGATTCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAGAAATTATTCTTGGCTTTATTT
GACAAGTATATGAAATGTCTAGAAATGACAAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCTA
TATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATTGTATAACGTTTATGCATATAATTATGTATAA
CTAGGCCTCAATTTCAAACAATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATCTTTAGAAAACC
CAAATATAGGGAACGATTGTGTTCATCAATATTGGAGATTCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTG
TATCTTCAAATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTTATTTTACACTGTCGGTGCATA
CGTATAAATTAAATGTAATTGAAAAAATGTCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAATC
CACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATATTGAATGCACCAGTCTCCAGGGATCGATTCAG
TACTCGAAAATTCTCAGAAGAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAGGGAAACTGCTG
CCAGAAGGCGTTGAGATTGC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50

Wildtype          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100

Wildtype         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCC-TTAGTCAC    149
                     ||||||||||||||||||||||||||||||||||||||||| ||||||||
Mutation         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCAC    150

Wildtype         150 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    200

Wildtype         200 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    250

Wildtype         250 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    300

Wildtype         300 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    350

Wildtype         350 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    400

Wildtype         400 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    450

Wildtype         450 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    500

Wildtype         500 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    550
gRNA                           ATGATAGATCCAGATGTTCC

Wildtype         550 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    600

Wildtype         600 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    650

Wildtype         650 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    700

Wildtype         700 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    750

Wildtype         750 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    800

Wildtype         800 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    850

Wildtype         850 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    900

Wildtype         900 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    950

Wildtype         950 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA   1000

Wildtype        1000 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1050

Wildtype        1050 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1100

Wildtype        1100 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1150

Wildtype        1150 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1200

Wildtype        1200 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1250

Wildtype        1250 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1300

Wildtype        1300 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1350

Wildtype        1350 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1400

Wildtype        1400 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1450

Wildtype        1450 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1500

Wildtype        1500 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1550

Wildtype        1550 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1600

Wildtype        1600 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1650

Wildtype        1650 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1700

Wildtype        1700 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1701 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1750

Wildtype        1750 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1800

Wildtype        1800 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1850

Wildtype        1850 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1900

Wildtype        1900 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1950

Wildtype        1950 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   2000

Wildtype        2000 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2050

Wildtype        2050 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2100

Wildtype        2100 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2150

Wildtype        2150 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2180
                     |||||||||||||||||||||||||||||||
Mutation        2151 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2181

Is tDNA present:

Mutation sequence:

ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGAAGTTGTTGATTATTTCTATCCAAGTGTTAA
GATGTCTGTTATTTATAACTCCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCACCTCTAAACCT
AGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCACTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATA
TTGTTCCCTACTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAGCATTGAGATGATTCATGGTC
GTAGACATGTTTAAGATTTATCTTAGACCACAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT
AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTATTTTTATATGAATATTTGACCACTTTTTTC
TTTTCTTTTTTCTATATTAATTGACAGATCATGATAGATCCAGATGTCCTGGTCCTAGTGATCCATATCTCAGGGAACAT
CTACACTGGTATTGATATATGACTTTAAACTAACTCATTCAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTAC
TTTGGAAGGGAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAAAAAAAAAAAAAATCCCATGAG
TTAGTTTGGAGTTTTCTTTTTTTGTACCTTTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATGG
GAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAGACATGAATGCATCAGCAATTCATAGAGATAT
GCTTATATATGTAGGAGTAGTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTCGTGTTTTCGTT
TGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAAGTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATA
TTTTTAAAATGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAAAAAAAAAGGTAGAGTAGAAAT
TTTCACATCAAACGCCTCCTAAATATGTCCAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGCA
GAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTTTAAGTGACATATTTGTGCAATATAATTGATA
GTTGACAAATACCTTAAAATAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACAGATTGCTCTTT
TGGTATGTATTCTTAACCCATACTTGGAAATAATATTTTTTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGAT
GGTATTGATTCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAGAAATTATTCTTGGCTTTATTT
GACAAGTATATGAAATGTCTAGAAATGACAAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCTA
TATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATTGTATAACGTTTATGCATATAATTATGTATAA
CTAGGCCTCAATTTCAAACAATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATCTTTAGAAAACC
CAAATATAGGGAACGATTGTGTTCATCAATATTGGAGATTCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTG
TATCTTCAAATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTTATTTTACACTGTCGGTGCATA
CGTATAAATTAAATGTAATTGAAAAAATGTCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAATC
CACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATATTGAATGCACCAGTCTCCAGGGATCGATTCAG
TACTCGAAAATTCTCAGAAGAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAGGGAAACTGCTG
CCAGAAGGCGTTGAGATTGC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGCTTCCAAAATGTGTGAACCCCTTGTGATTGGTAGAGTGATTGGTGA     50

Wildtype          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTTGTTGATTATTTCTATCCAAGTGTTAAGATGTCTGTTATTTATAACT    100

Wildtype         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCC-TTAGTCAC    149
                     ||||||||||||||||||||||||||||||||||||||||| ||||||||
Mutation         101 CCAACAAACATGTCTATAATGGGCATGAACTGTTTCCTTCCTTTAGTCAC    150

Wildtype         150 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTCTAAACCTAGGGTTGAAGTTCATGGTGGTGACCTCAGATCCTTCTTCA    200

Wildtype         200 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CTCTGGTATTTATTTAATACTTTACTAATCCCTTAAAATATTGTTCCCTA    250

Wildtype         250 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CTTTTACCCCCATCTGCCTTAATTTGTGTATGACACTCTTATTGTTTTAG    300

Wildtype         300 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CATTGAGATGATTCATGGTCGTAGACATGTTTAAGATTTATCTTAGACCA    350

Wildtype         350 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CAACTATAAAAGAATTTTTTGTCTTTTTTAAGCTTCATAACAACTCAATT    400

Wildtype         400 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AGTGCCACATGAATTGAAAACCGACGAAATAGTGCATTTGTCTTCTATTA    450

Wildtype         450 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TTTTTATATGAATATTTGACCACTTTTTTCTTTTCTTTTTTCTATATTAA    500

Wildtype         500 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TTGACAGATCATGATAGATCCAGATGTTCCTGGTCCTAGTGATCCATATC    550
gRNA                           ATGATAGATCCAGATGTTCC

Wildtype         550 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TCAGGGAACATCTACACTGGTATTGATATATGACTTTAAACTAACTCATT    600

Wildtype         600 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 CAATTATGTCTTGCCCCTTTTTTTTCCCAAAAAATTTTTACTTTGGAAGG    650

Wildtype         650 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAAAATGAAATGGTCATCCTATGATGTTTGGACTAAGAGTGTTTTTCGAA    700

Wildtype         700 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 AAAAAAAAAAAATCCCATGAGTTAGTTTGGAGTTTTCTTTTTTTGTACCT    750

Wildtype         750 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TTAATAAAGTTTAAAACACCATCGAGAACTACTATCTAACAAATTATATG    800

Wildtype         800 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GGAACACAATTAAGAGAAAGAAAGATCAACTGTTGCTGTTAATGTTTAAG    850

Wildtype         850 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 ACATGAATGCATCAGCAATTCATAGAGATATGCTTATATATGTAGGAGTA    900

Wildtype         900 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GTACAAATTAGCTAGGGTGCGTACGTTCAGAATGGAGTAGCCATTTTCTC    950

Wildtype         950 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 GTGTTTTCGTTTGGTTAAATTTATTTTAAAATATTTTTTTTGGATAATAA   1000

Wildtype        1000 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GTTCTTTAAAAGTAATAAAATGTAAATTACTTTTGGATATATTTTTAAAA   1050

Wildtype        1050 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 TGTTGATATTTTATGACCAATCATGTTTGTCGGAGTTCAACTCTGGAAAA   1100

Wildtype        1100 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 AAAAAAAGGTAGAGTAGAAATTTTCACATCAAACGCCTCCTAAATATGTC   1150

Wildtype        1150 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 CAATAATTGTCCTTAATCACTATAGTGAGAGTACCAAAAAAGAACACTGC   1200

Wildtype        1200 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGAATGATGATATTATTATATGTGGTAACTGAAAAGACTTACTCTCTGTT   1250

Wildtype        1250 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TAAGTGACATATTTGTGCAATATAATTGATAGTTGACAAATACCTTAAAA   1300

Wildtype        1300 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 TAGGATTTGACAATTTTTACAGGATTGTCACAGACATTCCAGGCACTACA   1350

Wildtype        1350 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 GATTGCTCTTTTGGTATGTATTCTTAACCCATACTTGGAAATAATATTTT   1400

Wildtype        1400 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 TTTTAATTTTATTTTACTTGTCTAATTCGTCTGATGGTGATGGTATTGAT   1450

Wildtype        1450 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 TCTTAATTTTTTTTTATAGTAATGATTAACAACCTGATTTCCTAAATGAG   1500

Wildtype        1500 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AAATTATTCTTGGCTTTATTTGACAAGTATATGAAATGTCTAGAAATGAC   1550

Wildtype        1550 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 AAAGTCAAAATTCCACTTCCAGATTGCAAAAAACAATATTTAACCTAGCT   1600

Wildtype        1600 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 ATATATATGGTTTTATTTTTTTAGTAAATTGACCTCCCTTTGACATCATT   1650

Wildtype        1650 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 GTATAACGTTTATGCATATAATTATGTATAACTAGGCCTCAATTTCAAAC   1700

Wildtype        1700 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1701 AATGTTAGTCTGCTTAATTTAAATAATATAGTAACGAAAATATATGAATC   1750

Wildtype        1750 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 TTTAGAAAACCCAAATATAGGGAACGATTGTGTTCATCAATATTGGAGAT   1800

Wildtype        1800 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TCATTTAGCTTGCATCTCCAAGTCTGTAGAACTGTTGTTTGTATCTTCAA   1850

Wildtype        1850 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 ATCACATACTTAATTAAGTTATCAAACTTTTCGGGGTTAAATAAAGATTT   1900

Wildtype        1900 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 ATTTTACACTGTCGGTGCATACGTATAAATTAAATGTAATTGAAAAAATG   1950

Wildtype        1950 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 TCAGGAAGAGAAGTGGTTGGGTATGAAATACCAAGGCCAAATATTGGAAT   2000

Wildtype        2000 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 CCACAGGTTTGTATTTTTGCTGTTCAAGCAGAAGAAAAGGCAAGCAATAT   2050

Wildtype        2050 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 TGAATGCACCAGTCTCCAGGGATCGATTCAGTACTCGAAAATTCTCAGAA   2100

Wildtype        2100 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 GAAAATGAGCTTGGTTCTCCTGTTGCTGCTGTTTTCTTCAACTGCCAGAG   2150

Wildtype        2150 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2180
                     |||||||||||||||||||||||||||||||
Mutation        2151 GGAAACTGCTGCCAGAAGGCGTTGAGATTGC   2181