Plant Crispr Database

Plant Information

Name: ncbp-1 #1

Seed stock:
Seed availability: No

Relationship:

Transformation: nCBP-1
transformation nCBP-1 to 60444

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
ncbp-1 #1 (T0 )	nCBP-1	nCBP-1	nCBP1-sgRNA	gene : Manes.09G140300 (nCBP-1)	-3 bp Is tDNA present: Mutation sequence: ATGGAGATCACAGAGGAGGAAACAGAAAACAATAACTATAGCAATAATAATGCTCGAACTGCATCATCACCGGATAATAT CGATAAAGTAGCCGAAGAACGCCAAGCAAGGGACCTCAAAGCTGGTTTGCATCCTCTCATTCTCGCTTCCTCTCTCCTTA TTTGAGTTTGTGTTGTGTAATTTTTTGTAGGTAGACATTCGATCCTTGCCAGTTTTTGAAATTTGATTATTTTGCGATAG ATTAATGGGATTCATTAATGTCATTCGGTGAATCTGTTTGTTAATTATTTTTAAGTGGTTGTATCTGCGGTTATGTTACA ATAAGAAAGAATTTTGATATGTTGTTTTTGAAATGCTAGCCAAATTATATCGTGATTCATAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 3306 TTATGAATCACGATATAATTTGGCTAGCATTTCAAAAACAACATATCAAA 3355 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TTATGAATCACGATATAATTTGGCTAGCATTTCAAAAACAACATATCAAA 50 Wildtype 3356 ATTCTTTCTTATTGTAACATAACCGCAGATACAACCACTTAAAAATAATT 3405 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 ATTCTTTCTTATTGTAACATAACCGCAGATACAACCACTTAAAAATAATT 100 Wildtype 3406 AACAAACAGATTCACCGAATGACATTAATGAATCCCATTAATCTATCGCA 3455 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AACAAACAGATTCACCGAATGACATTAATGAATCCCATTAATCTATCGCA 150 Wildtype 3456 AAATAATCAAATTTCAAAAACTGGCAAGGATCGAATGTCTACCTACAAAA 3505 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 AAATAATCAAATTTCAAAAACTGGCAAGGATCGAATGTCTACCTACAAAA 200 Wildtype 3506 AATTACACAACACAAACTCAAATAAGGAGAGAGGAAGCGAGAACC-TTGA 3554 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| MutationRev 201 AATTACACAACACAAACTCAAATAAGGAGAGAGGAAGCGAGAACCTTTGA 250 gRNA GAGGAAGCGAGAACC-TTGA Wildtype 3555 GAGGATGCAAACCAGCTTTGAGGTCCCTTGCTTGGCGTTCTTCGGCTACT 3604 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GAGGATGCAAACCAGCTTTGAGGTCCCTTGCTTGGCGTTCTTCGGCTACT 300 gRNA GAGGAAGCGAGAACCTTGAGAGG Wildtype 3605 TTATCGATATTATCCGGTGATGATGCAGTTCGAGCATTATTATTGCTATA 3654 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 TTATCGATATTATCCGGTGATGATGCAGTTCGAGCATTATTATTGCTATA 350 Wildtype 3655 GTTATTGTTTTCTGTTTCCTCCTCTGTGATCTCCAT 3690 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GTTATTGTTTTCTGTTTCCTCCTCTGTGATCTCCAT 386	Symptom incidence development similar to wild-type

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

ncbp-1 #1 (T0 )

nCBP-1

nCBP1-sgRNA

gene : Manes.09G140300 (nCBP-1)

-3 bp

Is tDNA present:

Mutation sequence:

ATGGAGATCACAGAGGAGGAAACAGAAAACAATAACTATAGCAATAATAATGCTCGAACTGCATCATCACCGGATAATAT
CGATAAAGTAGCCGAAGAACGCCAAGCAAGGGACCTCAAAGCTGGTTTGCATCCTCTCATTCTCGCTTCCTCTCTCCTTA
TTTGAGTTTGTGTTGTGTAATTTTTTGTAGGTAGACATTCGATCCTTGCCAGTTTTTGAAATTTGATTATTTTGCGATAG
ATTAATGGGATTCATTAATGTCATTCGGTGAATCTGTTTGTTAATTATTTTTAAGTGGTTGTATCTGCGGTTATGTTACA
ATAAGAAAGAATTTTGATATGTTGTTTTTGAAATGCTAGCCAAATTATATCGTGATTCATAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3306 TTATGAATCACGATATAATTTGGCTAGCATTTCAAAAACAACATATCAAA   3355
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TTATGAATCACGATATAATTTGGCTAGCATTTCAAAAACAACATATCAAA     50

Wildtype        3356 ATTCTTTCTTATTGTAACATAACCGCAGATACAACCACTTAAAAATAATT   3405
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 ATTCTTTCTTATTGTAACATAACCGCAGATACAACCACTTAAAAATAATT    100

Wildtype        3406 AACAAACAGATTCACCGAATGACATTAATGAATCCCATTAATCTATCGCA   3455
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AACAAACAGATTCACCGAATGACATTAATGAATCCCATTAATCTATCGCA    150

Wildtype        3456 AAATAATCAAATTTCAAAAACTGGCAAGGATCGAATGTCTACCTACAAAA   3505
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 AAATAATCAAATTTCAAAAACTGGCAAGGATCGAATGTCTACCTACAAAA    200

Wildtype        3506 AATTACACAACACAAACTCAAATAAGGAGAGAGGAAGCGAGAACC-TTGA   3554
                     ||||||||||||||||||||||||||||||||||||||||||||| ||||
MutationRev      201 AATTACACAACACAAACTCAAATAAGGAGAGAGGAAGCGAGAACCTTTGA    250
gRNA                                               GAGGAAGCGAGAACC-TTGA

Wildtype        3555 GAGGATGCAAACCAGCTTTGAGGTCCCTTGCTTGGCGTTCTTCGGCTACT   3604
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 GAGGATGCAAACCAGCTTTGAGGTCCCTTGCTTGGCGTTCTTCGGCTACT    300
gRNA                 GAGGAAGCGAGAACCTTGAGAGG

Wildtype        3605 TTATCGATATTATCCGGTGATGATGCAGTTCGAGCATTATTATTGCTATA   3654
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TTATCGATATTATCCGGTGATGATGCAGTTCGAGCATTATTATTGCTATA    350

Wildtype        3655 GTTATTGTTTTCTGTTTCCTCCTCTGTGATCTCCAT   3690
                     ||||||||||||||||||||||||||||||||||||
MutationRev      351 GTTATTGTTTTCTGTTTCCTCCTCTGTGATCTCCAT    386

Symptom incidence development similar to wild-type