Is tDNA present: No

Mutation sequence:

ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACATGGCTAT
ATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAAAAACACAAGG
GCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTC
TTAACAGCGATCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAAT
AAATTCTGAACTGAAGTTATTAGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC
CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAGCTCAAC
TCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTT
AAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTT
TTTTAATC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        5027 ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGT   5076
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGT     50

Wildtype        5077 GGAACCTTAGTAGCTCAGTTACATGGCTATATCCACTATATCCATTCAGG   5126
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GGAACCTTAGTAGCTCAGTTACATGGCTATATCCACTATATCCATTCAGG    100

Wildtype        5127 TCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAA   5176
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAA    150

Wildtype        5177 TAAAACAAGGGCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGC   5226
                     .||.||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 AAACACAAGGGCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGC    200

Wildtype        5227 AAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGA   5276
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGA    250

Wildtype        5277 TCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTT   5326
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTT    300

Wildtype        5327 TCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTCGTAGCTGC   5376
                     |||||||||||||||||||||||||||||||||||||||||.||||||||
Mutation         301 TCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTAGTAGCTGC    350

Wildtype        5377 TTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC   5426
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC    400

Wildtype        5427 CGACAGCAGCTCCGGCGCCGGTGCCGTTAGAAGCTCGTGCGTTTACCACC   5476
                     |||||||||||||||||||||||||||||    |||||||||||||||||
Mutation         401 CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACC    446
gRNA                                           TTAGAAGCTCGTGCGTTTAC

Wildtype        5477 CCATGACGCTGATCTTTCTCTGAGCTCAACTCCTTCAAGATTGAAGATTG   5526
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         447 CCATGACGCTGATCTTTCTCTGAGCTCAACTCCTTCAAGATTGAAGATTG    496

Wildtype        5527 ACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCAT   5576
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         497 ACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCAT    546

Wildtype        5577 AACCAATCTTAAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATA   5626
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         547 AACCAATCTTAAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATA    596

Wildtype        5627 CAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATC   5674
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         597 CAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATC    644