Plant Crispr Database

Plant Information

Name: CRT0019-3-7

Seed stock: 5303
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0019-3

Transformation: CRT0019
transformation CRT0019 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0019-3-7 (T1 )	CRT0019	CTG0019-2	CGR0019-2	gene : Solyc00g174340 (PR1b)	-5 bp/-5 bp Is tDNA present: Yes Mutation sequence: GGGGTTGTTCAACATCTCATTGTTACTCACTTGTCTCATGGTATTAGCCATATTTCACTCTTGTGAGGCCCAAAATTCAC CCAAAGACTATCTTGCGGTTCATAACGATGCCCGTGCCCAAGTCGGAGTCGGGCCTATGTCTTGGGATGCCAACTTGGCA TCCCGAGCACAAAACTATGCCAACTCAAGAGCTGGTGATTGTAACTTGATTCATTCTGGTGCTGGGGAGAATCTTGCCAA GGGTGGTGGTGACTTCACGGGGAGGGCAGCCGTGCAATTGTGGGTGTCCGAGAGGCCAAGCTATAACTACGCTACCAACC AATGTGTTGGTGGAAAACAGTGTAGACATTATACTCAAGTAGTCTGGCGCAACTCAGTCCGACTAGGTTGTGGTCGGGCA CGTTGCAGGATGGTGGTTCATTTCTTGCAACTATGATCCTGTAGGCAACTGGATCGGACAACGTCCTTACTAAAATGATG TATACTTATGACATGTTGCTAGTATTAAATAAAATTCTCATATGAGACGTCGAGAAGTTAAAATTTAAGTTTGACATAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1 TTAGTAAGGACGTTGTCCGATCCAGTTGCCTACAGGATCATAGTTGCAAG 50 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 87 TTAGTAAGGACGTTGTCCGATCCAGTTGCCTACAGGATCATAGTTGCAAG 136 Wildtype 51 AAATGAACCACCATCCGTTGTTGCAACGTGCCCGACCACAACCTAGTCGG 100 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 137 AAATGAACCACCATCC-----TGCAACGTGCCCGACCACAACCTAGTCGG 181 gRNA TCCGTTGTTGCAACGTGCCC Wildtype 101 ACTGAGTTGCGCCAGACTACTTGAGTATAATGTCTACACTTTTTTCCACC 150 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\| MutationRev 182 ACTGAGTTGCGCCAGACTACTTGAGTATAATGTCTACACTGTTTTCCACC 231 Wildtype 151 AACACATTGGTTGGTAGCGTAGTTATAGCTTGGCCTCTCGGACACCCACA 200 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 232 AACACATTGGTTGGTAGCGTAGTTATAGCTTGGCCTCTCGGACACCCACA 281 Wildtype 201 ATTGCACGGCTGCCCTCCCCGTGAAGTCACCACCACCCTTGGCAAGATTC 250 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 282 ATTGCACGGCTGCCCTCCCCGTGAAGTCACCACCACCCTTGGCAAGATTC 331 Wildtype 251 TCCCCAGCACCAGAATGAATCAAGTTACAATCACCAGCTCTTGAGTTGGC 300 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 332 TCCCCAGCACCAGAATGAATCAAGTTACAATCACCAGCTCTTGAGTTGGC 381 Wildtype 301 ATAGTTTTGTGCTCGGGATGCCAAGTTGGCATCCCAAGACATAGGCCCGA 350 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 382 ATAGTTTTGTGCTCGGGATGCCAAGTTGGCATCCCAAGACATAGGCCCGA 431 Wildtype 351 CTCCGACTTGGGCACGGGCATCGTTATGAACCGCAAGATAGTCTTGGGGT 400 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\| MutationRev 432 CTCCGACTTGGGCACGGGCATCGTTATGAACCGCAAGATAGTCTTTGGGT 481 Wildtype 401 GAATTTTGGGCCTCACAAGAGTGAAATATGGCTAATACCATGAGACAAGT 450 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 482 GAATTTTGGGCCTCACAAGAGTGAAATATGGCTAATACCATGAGACAAGT 531 Wildtype 451 GAGTAACAATGAGATGTTGAACAACCCC 478 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 532 GAGTAACAATGAGATGTTGAACAACCCC 559

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0019-3-7 (T1 )

CRT0019

CTG0019-2

CGR0019-2

gene : Solyc00g174340 (PR1b)

-5 bp/-5 bp

Is tDNA present: Yes

Mutation sequence:

GGGGTTGTTCAACATCTCATTGTTACTCACTTGTCTCATGGTATTAGCCATATTTCACTCTTGTGAGGCCCAAAATTCAC
CCAAAGACTATCTTGCGGTTCATAACGATGCCCGTGCCCAAGTCGGAGTCGGGCCTATGTCTTGGGATGCCAACTTGGCA
TCCCGAGCACAAAACTATGCCAACTCAAGAGCTGGTGATTGTAACTTGATTCATTCTGGTGCTGGGGAGAATCTTGCCAA
GGGTGGTGGTGACTTCACGGGGAGGGCAGCCGTGCAATTGTGGGTGTCCGAGAGGCCAAGCTATAACTACGCTACCAACC
AATGTGTTGGTGGAAAACAGTGTAGACATTATACTCAAGTAGTCTGGCGCAACTCAGTCCGACTAGGTTGTGGTCGGGCA
CGTTGCAGGATGGTGGTTCATTTCTTGCAACTATGATCCTGTAGGCAACTGGATCGGACAACGTCCTTACTAAAATGATG
TATACTTATGACATGTTGCTAGTATTAAATAAAATTCTCATATGAGACGTCGAGAAGTTAAAATTTAAGTTTGACATAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 TTAGTAAGGACGTTGTCCGATCCAGTTGCCTACAGGATCATAGTTGCAAG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       87 TTAGTAAGGACGTTGTCCGATCCAGTTGCCTACAGGATCATAGTTGCAAG    136

Wildtype          51 AAATGAACCACCATCCGTTGTTGCAACGTGCCCGACCACAACCTAGTCGG    100
                     ||||||||||||||||     |||||||||||||||||||||||||||||
MutationRev      137 AAATGAACCACCATCC-----TGCAACGTGCCCGACCACAACCTAGTCGG    181
gRNA                              TCCGTTGTTGCAACGTGCCC

Wildtype         101 ACTGAGTTGCGCCAGACTACTTGAGTATAATGTCTACACTTTTTTCCACC    150
                     ||||||||||||||||||||||||||||||||||||||||.|||||||||
MutationRev      182 ACTGAGTTGCGCCAGACTACTTGAGTATAATGTCTACACTGTTTTCCACC    231

Wildtype         151 AACACATTGGTTGGTAGCGTAGTTATAGCTTGGCCTCTCGGACACCCACA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      232 AACACATTGGTTGGTAGCGTAGTTATAGCTTGGCCTCTCGGACACCCACA    281

Wildtype         201 ATTGCACGGCTGCCCTCCCCGTGAAGTCACCACCACCCTTGGCAAGATTC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      282 ATTGCACGGCTGCCCTCCCCGTGAAGTCACCACCACCCTTGGCAAGATTC    331

Wildtype         251 TCCCCAGCACCAGAATGAATCAAGTTACAATCACCAGCTCTTGAGTTGGC    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      332 TCCCCAGCACCAGAATGAATCAAGTTACAATCACCAGCTCTTGAGTTGGC    381

Wildtype         301 ATAGTTTTGTGCTCGGGATGCCAAGTTGGCATCCCAAGACATAGGCCCGA    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      382 ATAGTTTTGTGCTCGGGATGCCAAGTTGGCATCCCAAGACATAGGCCCGA    431

Wildtype         351 CTCCGACTTGGGCACGGGCATCGTTATGAACCGCAAGATAGTCTTGGGGT    400
                     |||||||||||||||||||||||||||||||||||||||||||||.||||
MutationRev      432 CTCCGACTTGGGCACGGGCATCGTTATGAACCGCAAGATAGTCTTTGGGT    481

Wildtype         401 GAATTTTGGGCCTCACAAGAGTGAAATATGGCTAATACCATGAGACAAGT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      482 GAATTTTGGGCCTCACAAGAGTGAAATATGGCTAATACCATGAGACAAGT    531

Wildtype         451 GAGTAACAATGAGATGTTGAACAACCCC    478
                     ||||||||||||||||||||||||||||
MutationRev      532 GAGTAACAATGAGATGTTGAACAACCCC    559