Plant Crispr Database

Plant Information

Name: CRT0024-5-6

Seed stock: 5437
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0024-5

Transformation: CRT0024
transformation CRT0024 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0024-5-6 (T1 )	CRT0024	CTG0024-2	CGR0024-2	gene : Solyc12g017830 (Min7)	azygous Is tDNA present: No Mutation sequence: ATATTTCATAGATTGAATATCTAAGAACATTCTTTCGGGTGCTCAGAGATTCTCAAAAAGCGTATCCAAAAATCAGTGAT AACTTTCTCTGTATATCTGCAGAAACTGATTGCCTACGACCATCTAGAGGGAGATCTTGGCTTAGATGGTGGGGAAAATG TGACGCTGTTTACAGACATCCTGAACAGGGTCTGTGGCTGTGTGGATAATTTGTCTACCGATAGGTAGAAACCTCCCTTT TTTGACTTTTTCTTATGTATATTTCCTTTTAAGTTATCAACAAATCGTTTGCTCCTGTTAACTATGTGCCTCTTTTATTT AATATATATTTTAACTTAATATTTCTTGAATGATAAATCTCTTGAGGATTTCTATGCAGTACGACTCTTCAGGTTCTGAA GGTACTTCTTACCGCTGTAGCATCCGCCAAGTTTAGAGGTATGCAACTTTTGAAAGAGATTCATTTCTTCACCTTTTGTT ACTCTTGAGACTTGTCCCAAGTTACTTTATGTCTTTACCCTCTTTCTATCTTGACTTTGTTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 6858 ATATTTCATAGATTGAATATCTAAGAACATTCTTTCGGGTGCTCAGAGAT 6907 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 ATATTTCATAGATTGAATATCTAAGAACATTCTTTCGGGTGCTCAGAGAT 50 Wildtype 6908 TCTCAAAAAGCGTATCCAAAAATCAGTGATAACTTTCTCTGTATATCTGC 6957 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 TCTCAAAAAGCGTATCCAAAAATCAGTGATAACTTTCTCTGTATATCTGC 100 Wildtype 6958 AGAAACTGATTGCCTACGACCATCTAGAGGGAGATCTTGGCTTAGATGGT 7007 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 AGAAACTGATTGCCTACGACCATCTAGAGGGAGATCTTGGCTTAGATGGT 150 Wildtype 7008 GGGGAAAATGTGACGCTGTTTACAGACATCCTGAACAGGGTCTGTGGCTG 7057 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 GGGGAAAATGTGACGCTGTTTACAGACATCCTGAACAGGGTCTGTGGCTG 200 gRNA GAACAGGGTCTGTGGCTG Wildtype 7058 TGTGGATAATTTGTCTACCGATAGGTAGAAACCTCCCTTTTTTGACTTTT 7107 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 TGTGGATAATTTGTCTACCGATAGGTAGAAACCTCCCTTTTTTGACTTTT 250 gRNA GAACAGGGTCTGTGGCTGTG Wildtype 7108 TCTTATGTATATTTCCTTTTAAGTTATCAACAAATCGTTTGCTCCTGTTA 7157 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 TCTTATGTATATTTCCTTTTAAGTTATCAACAAATCGTTTGCTCCTGTTA 300 Wildtype 7158 ACTATGTGCCTCTTTTATTTAATATATATTTTAACTTAATATTTCTTGAA 7207 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 301 ACTATGTGCCTCTTTTATTTAATATATATTTTAACTTAATATTTCTTGAA 350 Wildtype 7208 TGATAAATCTCTTGAGGATTTCTATGCAGTACGACTCTTCAGGTTCTGAA 7257 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 351 TGATAAATCTCTTGAGGATTTCTATGCAGTACGACTCTTCAGGTTCTGAA 400 Wildtype 7258 GGTACTTCTTACCGCTGTAGCATCCGCCAAGTTTAGAGGTATGCAACTTT 7307 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 401 GGTACTTCTTACCGCTGTAGCATCCGCCAAGTTTAGAGGTATGCAACTTT 450 Wildtype 7308 TGAAAGAGATTCATTTCTTCACCTTTTGTTACTCTTGAGACTTGTCCCAA 7357 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 451 TGAAAGAGATTCATTTCTTCACCTTTTGTTACTCTTGAGACTTGTCCCAA 500 Wildtype 7358 GTTACTTTATGTCTTTACCCTCTTTCTATCTTGACTTTGTTC 7399 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 501 GTTACTTTATGTCTTTACCCTCTTTCTATCTTGACTTTGTTC 542

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0024-5-6 (T1 )

CRT0024

CTG0024-2

CGR0024-2

gene : Solyc12g017830 (Min7)

azygous

Is tDNA present: No

Mutation sequence:

ATATTTCATAGATTGAATATCTAAGAACATTCTTTCGGGTGCTCAGAGATTCTCAAAAAGCGTATCCAAAAATCAGTGAT
AACTTTCTCTGTATATCTGCAGAAACTGATTGCCTACGACCATCTAGAGGGAGATCTTGGCTTAGATGGTGGGGAAAATG
TGACGCTGTTTACAGACATCCTGAACAGGGTCTGTGGCTGTGTGGATAATTTGTCTACCGATAGGTAGAAACCTCCCTTT
TTTGACTTTTTCTTATGTATATTTCCTTTTAAGTTATCAACAAATCGTTTGCTCCTGTTAACTATGTGCCTCTTTTATTT
AATATATATTTTAACTTAATATTTCTTGAATGATAAATCTCTTGAGGATTTCTATGCAGTACGACTCTTCAGGTTCTGAA
GGTACTTCTTACCGCTGTAGCATCCGCCAAGTTTAGAGGTATGCAACTTTTGAAAGAGATTCATTTCTTCACCTTTTGTT
ACTCTTGAGACTTGTCCCAAGTTACTTTATGTCTTTACCCTCTTTCTATCTTGACTTTGTTC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        6858 ATATTTCATAGATTGAATATCTAAGAACATTCTTTCGGGTGCTCAGAGAT   6907
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATATTTCATAGATTGAATATCTAAGAACATTCTTTCGGGTGCTCAGAGAT     50

Wildtype        6908 TCTCAAAAAGCGTATCCAAAAATCAGTGATAACTTTCTCTGTATATCTGC   6957
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TCTCAAAAAGCGTATCCAAAAATCAGTGATAACTTTCTCTGTATATCTGC    100

Wildtype        6958 AGAAACTGATTGCCTACGACCATCTAGAGGGAGATCTTGGCTTAGATGGT   7007
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AGAAACTGATTGCCTACGACCATCTAGAGGGAGATCTTGGCTTAGATGGT    150

Wildtype        7008 GGGGAAAATGTGACGCTGTTTACAGACATCCTGAACAGGGTCTGTGGCTG   7057
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GGGGAAAATGTGACGCTGTTTACAGACATCCTGAACAGGGTCTGTGGCTG    200
gRNA                                                 GAACAGGGTCTGTGGCTG

Wildtype        7058 TGTGGATAATTTGTCTACCGATAGGTAGAAACCTCCCTTTTTTGACTTTT   7107
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TGTGGATAATTTGTCTACCGATAGGTAGAAACCTCCCTTTTTTGACTTTT    250
gRNA                 GAACAGGGTCTGTGGCTGTG

Wildtype        7108 TCTTATGTATATTTCCTTTTAAGTTATCAACAAATCGTTTGCTCCTGTTA   7157
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TCTTATGTATATTTCCTTTTAAGTTATCAACAAATCGTTTGCTCCTGTTA    300

Wildtype        7158 ACTATGTGCCTCTTTTATTTAATATATATTTTAACTTAATATTTCTTGAA   7207
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 ACTATGTGCCTCTTTTATTTAATATATATTTTAACTTAATATTTCTTGAA    350

Wildtype        7208 TGATAAATCTCTTGAGGATTTCTATGCAGTACGACTCTTCAGGTTCTGAA   7257
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TGATAAATCTCTTGAGGATTTCTATGCAGTACGACTCTTCAGGTTCTGAA    400

Wildtype        7258 GGTACTTCTTACCGCTGTAGCATCCGCCAAGTTTAGAGGTATGCAACTTT   7307
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 GGTACTTCTTACCGCTGTAGCATCCGCCAAGTTTAGAGGTATGCAACTTT    450

Wildtype        7308 TGAAAGAGATTCATTTCTTCACCTTTTGTTACTCTTGAGACTTGTCCCAA   7357
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TGAAAGAGATTCATTTCTTCACCTTTTGTTACTCTTGAGACTTGTCCCAA    500

Wildtype        7358 GTTACTTTATGTCTTTACCCTCTTTCTATCTTGACTTTGTTC   7399
                     ||||||||||||||||||||||||||||||||||||||||||
Mutation         501 GTTACTTTATGTCTTTACCCTCTTTCTATCTTGACTTTGTTC    542