Is tDNA present: No

Mutation sequence:

GTACCTTCTTTTAAATGTAATTCATTCATTGTACTATCCTAACTGTGGGGACACTTCTGCTTGTTGCCAGAGGACTTGGA
GATAATAGGTTGAGCCCACTGTCACGAAGCCCAGTTCGTTCCAGAGGGACAACTACCACATCATCACCTTTACATCCTCG
GTTTTCGAGTTTAAATCTGGACTCTTCAACGGGCAAGTTGGATGATGTAAGGAGTGAATGTCATCAGCTGCCCCTTCCAC
CCGGTTCTCCAC----TCCTTCTGCTTTGCCAAACCCAAGACCTTGTGTTGTGGCTGAAGGCGCAAACGTTAATATGTCA
AAATGGAAGAAAGGCAGGCTCCTAGGAAGAGGCACGTTTGGTCATGTGTATCTTGGATTCAACAGGTTTGTGAGCCTTCC
AGCTTTTTTTCGATCAATGACGTTTGGGTTCTAACTAGGTTCAGGAGCATCATGGCTT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1247 GTACCTTCTTTTAAATGTAATTCATTCATTGTACTATCCTAACTGTGGGG   1296
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 GTACCTTCTTTTAAATGTAATTCATTCATTGTACTATCCTAACTGTGGGG     50

Wildtype        1297 ACACTTCTGCTTGTTGCCAGAGGACTTGGAGATAATAGGTTGAGCCCACT   1346
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ACACTTCTGCTTGTTGCCAGAGGACTTGGAGATAATAGGTTGAGCCCACT    100

Wildtype        1347 GTCACGAAGCCCAGTTCGTTCCAGAGGGACAACTACCACATCATCACCTT   1396
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 GTCACGAAGCCCAGTTCGTTCCAGAGGGACAACTACCACATCATCACCTT    150

Wildtype        1397 TACATCCTCGGTTTTCGAGTTTAAATCTGGACTCTTCAACGGGCAAGTTG   1446
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TACATCCTCGGTTTTCGAGTTTAAATCTGGACTCTTCAACGGGCAAGTTG    200

Wildtype        1447 GATGATGTAAGGAGTGAATGTCATCAGCTGCCCCTTCCACCCGGTTCTCC   1496
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 GATGATGTAAGGAGTGAATGTCATCAGCTGCCCCTTCCACCCGGTTCTCC    250

Wildtype        1497 ACCCAGTCCTTCTGCTTTGCCAAACCCAAGACCTTGTGTTGTGGCTGAAG   1546
                     ||    ||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AC----TCCTTCTGCTTTGCCAAACCCAAGACCTTGTGTTGTGGCTGAAG    296
gRNA                  CCCAGTCCTTCTGCTTTGCC

Wildtype        1547 GCGCAAACGTTAATATGTCAAAATGGAAGAAAGGCAGGCTCCTAGGAAGA   1596
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         297 GCGCAAACGTTAATATGTCAAAATGGAAGAAAGGCAGGCTCCTAGGAAGA    346

Wildtype        1597 GGCACGTTTGGTCATGTGTATCTTGGATTCAACAGGTTTGTGAGCCTTCC   1646
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         347 GGCACGTTTGGTCATGTGTATCTTGGATTCAACAGGTTTGTGAGCCTTCC    396

Wildtype        1647 AGCTTTTTTTCGATCAATGACGTTTGGGTTCTAACTAGGTTCAGGAGCAT   1696
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         397 AGCTTTTTTTCGATCAATGACGTTTGGGTTCTAACTAGGTTCAGGAGCAT    446

Wildtype        1697 CATGGCTT   1704
                     ||||||||
Mutation         447 CATGGCTT    454