Is tDNA present: Yes

Mutation sequence:

GGGCATTTTCTTCTTGTCCTTTACATCTACTTTTCTCATTTGTCAAACTTGTATGTTAACTTTATCTTCTACTACTATGC
TTATTTATATTAAATGTTAGCTATGCCTTTAGGAATATCTCAAATTCTCCAAAGGCCAAGAGAGCCAAGTTTCCTTGTGA
GTTACAATTTTTATTCTTTTATAGCATATACTACAAAAAGTTCAAATCTTTTTGCTTTATTATTCAAGATTGTATT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         592 GGCATTTTCTCTGCTTCTGCAACCATTGTTTTCCAGTACTTAGTGAGGAC    641
                     ||||||||||                                        
Mutation           2 GGCATTTTCT----------------------------------------     11

Wildtype         642 TCTTTGGTGCTTGACAATTTCTTGTCTGCTTTTGACAGCATCCCTGCCTC    691
                     ||||  ||.|||.|||        |||.|||||                 
Mutation          12 TCTT--GTCCTTTACA--------TCTACTTTT-----------------     34

Wildtype         692 GATACTCACTGCGTTTGTAACTATCATAGTAGCCTCCTTCTTTCAGGGTT    741
                         ||||     ||||      |||.|        |||.|.|    |||
Mutation          35 ----CTCA-----TTTG------TCAAA--------CTTGTAT----GTT     57

Wildtype         742 -ACTT--TCTTGTACCACTCTAGCCATGTCATAGCTTCTTTCATTT----    784
                      ||||  ||||.|||.|||.|            ||||.||| ||.|    
Mutation          58 AACTTTATCTTCTACTACTAT------------GCTTATTT-ATATTAAA     94

Wildtype         785 TGTTTAGCT---TCTTCCCAGGA-----TCAAAGGCATTCCTCTTCTTTG    826
                     || ||||||   .|||  .||||     |||||          |||  |.
Mutation          95 TG-TTAGCTATGCCTT--TAGGAATATCTCAAA----------TTC--TC    129

Wildtype         827 CAAA-------AGATTCTTCCACTCTTTTCTTCACTTTAGTCCTTATAGA    869
                     ||||       |||    .|||           |.||   |||||.| ||
Mutation         130 CAAAGGCCAAGAGA----GCCA-----------AGTT---TCCTTGT-GA    160

Wildtype         870 GTTCATGTTACTGGTCTGCTGCCACGACAAAATATATGCATTGTAGAAGT    919
                     |||                       ||||..|.|||.| ||.||     
Mutation         161 GTT-----------------------ACAATTTTTATTC-TTTTA-----    181

Wildtype         920 TAG-AAATACAGGTTTAGAAACAAGTTCTATAAGT-TTTTCGAATG-TTT    966
                     ||| |.||||     ||.||| ||||||   ||.| ||||    || |||
Mutation         182 TAGCATATAC-----TACAAA-AAGTTC---AAATCTTTT----TGCTTT    218

Wildtype         967 ATCTCTACT---GATTTCCTATT    986
                     ||   ||.|   ||||  .||||
Mutation         219 AT---TATTCAAGATT--GTATT    236