| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA2 |
AGO7-sgRNA2 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA2 |
AGO7-sgRNA2 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA2 |
AGO7-sgRNA2 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA3 |
AGO7-sgRNA3 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA3 |
AGO7-sgRNA3 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| Ppr-ago7CR-a2 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA3 |
AGO7-sgRNA3 |
mRNA : Ppr-t_75930 (AGO7) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
