| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA2 |
AGO7-sgRNA2 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA2 |
AGO7-sgRNA2 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA2 |
AGO7-sgRNA2 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA3 |
AGO7-sgRNA3 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA3 |
AGO7-sgRNA3 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| Ppr-ago7CR-a6 (T0 ) |
AGO7-sgRNA1 |
AGO7-sgRNA3 |
AGO7-sgRNA3 |
mRNA : Ppr-t_75930 (AGO7) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
