Is tDNA present:

Mutation sequence:

ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAATAATAATGCTCAAACGACATTGGATTCAGC
ATCACTTGAGAACATAGACAAAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCTCTCAGGTTCT
CTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTCTATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTT
GATTATTTTGCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGATTTTTGTATTTTTTTTTTCCT
GAAGTGGGTTATACTGGATTTATGCTACTAAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAA     50

Wildtype          51 TAATAATGCTCAAACGACATTGGATTCAGCATCACTTGAGAACATAGACA    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TAATAATGCTCAAACGACATTGGATTCAGCATCACTTGAGAACATAGACA    100

Wildtype         101 AAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||  
Mutation         101 AAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATC--    148
gRNA                                                                CCT

Wildtype         151 CTCAAGGTTCTCTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTC    200
                              |||||||||||||||||||||||||||||||||||||||||
Mutation         149 ---------CTCTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTC    189
gRNA                 CCTCTCAAGGTTCTCTCTTTCGC

Wildtype         201 TATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTTGATTATTTT    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         190 TATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTTGATTATTTT    239

Wildtype         251 GCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGA    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         240 GCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGA    289

Wildtype         301 TTTTTGTATTTTTTTTTTCCTGAAGTGGGTTATACTGGATTTATGCTACT    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         290 TTTTTGTATTTTTTTTTTCCTGAAGTGGGTTATACTGGATTTATGCTACT    339

Wildtype         351 AAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA    385
                     |||||||||||||||||||||||||||||||||||
Mutation         340 AAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA    374