GCATTATATGTGTGGAGGGCGGGGGTGATGTAGAATCTGTAGATTGTTCTTTTCTGTTTGGCAGCCATAAAGAGAACCAA
GTTTCTAATTATATAGTTGTGCTCTGTATATCAGGCAAGACCATTGTTCAAAGATCGGAGGAAGTTCTCACAGATGGCTG
ATCCAATGCTACAAGGCCATTATCCAGTAAGAGGCATGTACCAAGCTCTAGCAGTTGCAGCAATGTGTGTTCAGGAGCAA
CCTAACAATGCGTCCAGTCATAGCAGACGTTGTCACGGCTTTGACTTACCTTGCTTCACAAAAGTTTGACCATGAAACCC
GAGGGGGCATCCAAACTTCACGGCCAGGACCTGCTACTTCACCTAGAATG

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1194 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA   1243
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA     50

Wildtype        1244 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT   1293
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT    100

Wildtype        1294 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA   1343
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA    150

Wildtype        1344 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC   1393
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC    200

Wildtype        1394 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT   1443
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT    250

Wildtype        1444 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC   1493
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC    300

Wildtype        1494 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA   1543
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA    350

Wildtype        1544 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG   1593
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG    400

Wildtype        1594 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC   1643
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC    450

Wildtype        1644 CCTGCAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT   1693
                     ||||| ||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 CCTGC-ATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT    499

Wildtype        1694 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG   1743
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         500 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG    549

Wildtype        1744 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT   1793
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         550 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT    599

Wildtype        1794 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT   1843
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         600 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT    649

Wildtype        1844 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC   1893
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         650 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC    699

Wildtype        1894 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA   1943
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         700 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA    749

Wildtype        1944 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA   1993
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         750 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA    799

Wildtype        1994 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA   2043
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         800 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA    849

Wildtype        2044 AAAC   2047
                     ||||
Mutation         850 AAAC    853

TTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGGAGTAGCAGGTGTCCAGCGTGGGTTTTGAACTCCCTCAGTATCAC
TGTCAAATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACTGGACGCATGTTAGGTTGTTCCTGA
ACACACATTGCTGCAACTGCTAGAGCTTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTGGATCAGCCATCTG
TGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGAGAGCATGAGTTTAATATTTGAACTTGGTTCTATA
AAATTGGTTTTCTAATAGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTCTATAGTTTCTTGGC
CTTCGATTTTTCTTAATATCTATTGTCCTTGTACCATAATTATCGAATTTGTTTTGATGTAGTTTGTGTTCTATTACTAC
CTGTTTTCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGACTGCTTTGGATTGTTTTTCTTTTG
AGCCAGGGGTCTATCGGAAACC

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3873 CTATGCGACAAAATAGCAAGTTCAACCAAAATCTGGTGGATTGGGGGCAT   3922
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CTATGCGACAAAATAGCAAGTTCAACCAAAATCTGGTGGATTGGGGGCAT     50

Wildtype        3923 TACCAGATGGAGACCGAAAAAAGAACTTCATTCCATTATTGAAAAGAAAA   3972
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TACCAGATGGAGACCGAAAAAAGAACTTCATTCCATTATTGAAAAGAAAA    100

Wildtype        3973 AAGTGGCAGAAAAGACCTGATTGGAATCGAGCCGCCCTTTATATACTCTT   4022
                     |||||.||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AAGTGACAGAAAAGACCTGATTGGAATCGAGCCGCCCTTTATATACTCTT    150

Wildtype        4023 CCGAATCCTCCCTCTCCAAGAAGACAATCACCTCGAAAGTTTCTTGTTGC   4072
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CCGAATCCTCCCTCTCCAAGAAGACAATCACCTCGAAAGTTTCTTGTTGC    200

Wildtype        4073 AACTGCTAATTCACGAAATTTAAAAATCTTGGCTCCAATTTGACCAGACT   4122
                     |||||||||||||||||||||||||||||||||||||| |||||||||||
Mutation         201 AACTGCTAATTCACGAAATTTAAAAATCTTGGCTCCAA-TTGACCAGACT    249

Wildtype        4123 CGCCATCCTTAACATACTCCTTTGAACCTAACAGAGTCTTTGCCTTCAAC   4172
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 CGCCATCCTTAACATACTCCTTTGAACCTAACAGAGTCTTTGCCTTCAAC    299

Wildtype        4173 TTATCTGAACACGAAAAAAAGAGAAAT   4199
                     |||||||||||||||||||||||||||
Mutation         300 TTATCTGAACACGAAAAAAAGAGAAAT    326