Is tDNA present: Yes

Mutation sequence:

ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGAAACCAACGCCACAACCAAGGAGAATTCTTC
CAATAATAAGCATAGCTAAATCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGCGATTAACATA
GTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAAAAAACGTCGCCGTTAACCAGCTAGGTATAACGATGAAGTA
AATAATTGTAACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTTTGTTCTCTTATAAACTGTTGG
AAAGAATTTTTTTAAAAATGGATCCATTGATGTAACACCACCTGTATTTTTTTAAAAAAAAAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4633 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA   4682
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA     50

Wildtype        4683 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA   4732
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA    100

Wildtype        4733 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC   4782
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC    150

Wildtype        4783 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA   4832
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA    200

Wildtype        4833 AAAACGTCGCCGTTAAACCAGCTAGGTATAACGATGAAGTAAATAATTGT   4882
                     |||||||||||||| |||||||||||||||||||||||||||||||||||
Mutation         201 AAAACGTCGCCGTT-AACCAGCTAGGTATAACGATGAAGTAAATAATTGT    249
gRNA                             TTAAACCAGCTAGGTATAAC

Wildtype        4883 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT   4932
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT    299

Wildtype        4933 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG   4982
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG    349

Wildtype        4983 ATGTAACACCACCTGTATTTTT   5004
                     ||||||||||||||||||||||
Mutation         350 ATGTAACACCACCTGTATTTTT    371