Plant Crispr Database

Plant Information

Name: CRT0038-1-2

Seed stock: 5040
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0038-1

Transformation: CRT0038
transformation CRT0038 to RG-pto11

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0038-1-2 (T1 )	CRT0038	CTG0037-1	CGR0037-1	gene : Solyc05g013290 (Fen)	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: CTGTGAGTTTTCGATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGT ATTCCAAGGCAACAAATTCGATAAATGATGCTTCTAACTTGATTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTG TAGATTTGGAGGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGT GTTTTGCGTGATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGA AATTGAGATTCTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTC TAATTTATGACTACATGGAGAATGGGAACCTCAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 596 TTGAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCT 645 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TTGAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCT 50 Wildtype 646 TTCATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATG 695 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TTCATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATG 100 Wildtype 696 AGAGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCA 745 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AGAGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCA 150 Wildtype 746 CGTTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCT 795 .\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GGTTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCT 200 Wildtype 796 GTAAACCTTCCCAAATCCACCCTCTCCAATGAAAAACTTGTCATCAAAGT 845 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GTAAACCTTCCCAAATCCACCCTCTCCAATGAAAAAATTGTCATCAAAGT 250 Wildtype 846 TGTTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAA 895 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TGTTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAA 300 Wildtype 896 GGAATGCCATAACTCAAGTTAGAAGCATCATTTATGGAATTTGTTGCCTT 945 \|\|\|\|.\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 GGAACGCCATAA-TCAAGTTAGAAGCATCATTTATCGAATTTGTTGCCTT 349 gRNA TAACTCAAGTTAGAAGCATC Wildtype 946 GGAATACTTGCTTCCCAT 963 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 350 GGAATACTTGCTTCCCAT 367	<b>Morphology : </b><a href=/images/phenotype/68/CRT0038.pdf target=_blank><span class="glyphicon glyphicon-picture"</span></a><br><b>Immunity response: </b>More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB)<br>

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0038-1-2 (T1 )

CRT0038

CTG0037-1

CGR0037-1

gene : Solyc05g013290 (Fen)

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

CTGTGAGTTTTCGATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGT
ATTCCAAGGCAACAAATTCGATAAATGATGCTTCTAACTTGATTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTG
TAGATTTGGAGGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGT
GTTTTGCGTGATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGA
AATTGAGATTCTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTC
TAATTTATGACTACATGGAGAATGGGAACCTCAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         596 TTGAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCT    645
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TTGAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCT     50

Wildtype         646 TTCATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATG    695
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TTCATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATG    100

Wildtype         696 AGAGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCA    745
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AGAGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCA    150

Wildtype         746 CGTTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCT    795
                     .|||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GGTTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCT    200

Wildtype         796 GTAAACCTTCCCAAATCCACCCTCTCCAATGAAAAACTTGTCATCAAAGT    845
                     ||||||||||||||||||||||||||||||||||||.|||||||||||||
MutationRev      201 GTAAACCTTCCCAAATCCACCCTCTCCAATGAAAAAATTGTCATCAAAGT    250

Wildtype         846 TGTTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAA    895
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TGTTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAA    300

Wildtype         896 GGAATGCCATAACTCAAGTTAGAAGCATCATTTATGGAATTTGTTGCCTT    945
                     ||||.||||||| ||||||||||||||||||||||.||||||||||||||
MutationRev      301 GGAACGCCATAA-TCAAGTTAGAAGCATCATTTATCGAATTTGTTGCCTT    349
gRNA                          TAACTCAAGTTAGAAGCATC

Wildtype         946 GGAATACTTGCTTCCCAT    963
                     ||||||||||||||||||
MutationRev      350 GGAATACTTGCTTCCCAT    367

Morphology : <a href=/images/phenotype/68/CRT0038.pdf target=_blank></a> Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB)