Plant Crispr Database

Plant Information

Name: CRT0038-4-13-1

Seed stock: 5336
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0038-4-13

Transformation: CRT0038
transformation CRT0038 to RG-pto11

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0038-4-13-1 (T2 )	CRT0038	CTG0037-1	CGR0037-1	gene : Solyc05g013290 (Fen)	+1 bp/+1 bp Is tDNA present: Yes Mutation sequence: GATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGTATTCCAAGGCAA CAAATTCGATAAATGATGCTTCTAACTTGAGTTTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTGTAGATTTGGA GGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGTGTTTTGCGTG ATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGAAATTGAGATT CTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTCTAATTTATGA CTACATGGAGAATGGGAACCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 598 GAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCTTT 647 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 GAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCTTT 50 Wildtype 648 CATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATGAG 697 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATGAG 100 Wildtype 698 AGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCACG 747 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\| MutationRev 101 AGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCAGG 150 Wildtype 748 TTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCTGT 797 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCTGT 200 Wildtype 798 AAACCTTCCCAAATCCACCCTCTCCAATGAAAAACTTGTCATCAAAGTTG 847 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AAACCTTCCCAAATCCACCCTCTCCAATGAAAAAATTGTCATCAAAGTTG 250 Wildtype 848 TTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAAGG 897 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAAGG 300 Wildtype 898 AATGCCAT-AACTCAAGTTAGAAGCATCATTTATGGAATTTGTTGCCTTG 946 \|\|.\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 AACGCCATAAACTCAAGTTAGAAGCATCATTTATCGAATTTGTTGCCTTG 350 gRNA T-AACTCAAGTTAGAAGCATC Wildtype 947 GAATACTTGCTTCCCAT 963 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GAATACTTGCTTCCCAT 367	Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB)

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0038-4-13-1 (T2 )

CRT0038

CTG0037-1

CGR0037-1

gene : Solyc05g013290 (Fen)

+1 bp/+1 bp

Is tDNA present: Yes

Mutation sequence:

GATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGTATTCCAAGGCAA
CAAATTCGATAAATGATGCTTCTAACTTGAGTTTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTGTAGATTTGGA
GGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGTGTTTTGCGTG
ATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGAAATTGAGATT
CTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTCTAATTTATGA
CTACATGGAGAATGGGAACCTC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         598 GAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCTTT    647
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 GAGGTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCTTT     50

Wildtype         648 CATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATGAG    697
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATGAG    100

Wildtype         698 AGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCACG    747
                     ||||||||||||||||||||||||||||||||||||||||||||||||.|
MutationRev      101 AGAATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCAGG    150

Wildtype         748 TTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCTGT    797
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCTGT    200

Wildtype         798 AAACCTTCCCAAATCCACCCTCTCCAATGAAAAACTTGTCATCAAAGTTG    847
                     ||||||||||||||||||||||||||||||||||.|||||||||||||||
MutationRev      201 AAACCTTCCCAAATCCACCCTCTCCAATGAAAAAATTGTCATCAAAGTTG    250

Wildtype         848 TTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAAGG    897
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAAGG    300

Wildtype         898 AATGCCAT-AACTCAAGTTAGAAGCATCATTTATGGAATTTGTTGCCTTG    946
                     ||.||||| |||||||||||||||||||||||||.|||||||||||||||
MutationRev      301 AACGCCATAAACTCAAGTTAGAAGCATCATTTATCGAATTTGTTGCCTTG    350
gRNA                        T-AACTCAAGTTAGAAGCATC

Wildtype         947 GAATACTTGCTTCCCAT    963
                     |||||||||||||||||
MutationRev      351 GAATACTTGCTTCCCAT    367

Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB)