Plant Crispr Database

Plant Information

Name: CRT0074-5-1

Seed stock: 5366
Seed availability: No

Relationship:
Parent: CRT0074-5

Transformation: CRT0074
transformation CRT0074 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0074-5-1 (T1 )	CRT0074	CTG0074-1	CGR0074-1	gene : Solyc10g085990 (Mai5)	-11 bp/-11 bp Is tDNA present: Yes Mutation sequence: CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCATTT CAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTC TTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCA AATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGT AGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC AGCACTTCGACAAGAAATTCCCTGTTGCCCACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAA AGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAA TGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCCA ATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAAGAA GAAAAGCATTTTCCTTATTTGAGTAAGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 12 TCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCT 61 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCT 50 Wildtype 62 CGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGAC 111 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGAC 100 Wildtype 112 AACGTCTGCTATGACTGGACGCATGTTAGGTTGCTCCTGAACACACATTG 161 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AACGTCTGCTATGACT------------GGTTGCTCCTGAACACACATTG 138 Wildtype 162 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT 211 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 139 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT 188 Wildtype 212 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG 261 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 189 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG 238 Wildtype 262 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT 311 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 239 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT 288 Wildtype 312 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC 361 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 289 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC 338 Wildtype 362 GCCCTCCACACATA 375 \|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 339 GCCCTCCACACATA 352

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0074-5-1 (T1 )

CRT0074

CTG0074-1

CGR0074-1

gene : Solyc10g085990 (Mai5)

-11 bp/-11 bp

Is tDNA present: Yes

Mutation sequence:

CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCATTT
CAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTC
TTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCA
AATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGT
AGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC
AGCACTTCGACAAGAAATTCCCTGTTGCCCACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAA
AGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAA
TGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCCA
ATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAAGAA
GAAAAGCATTTTCCTTATTTGAGTAAGG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          12 TCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCT     61
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCT     50

Wildtype          62 CGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGAC    111
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGAC    100

Wildtype         112 AACGTCTGCTATGACTGGACGCATGTTAGGTTGCTCCTGAACACACATTG    161
                     ||||||||||||||||            ||||||||||||||||||||||
MutationRev      101 AACGTCTGCTATGACT------------GGTTGCTCCTGAACACACATTG    138

Wildtype         162 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT    211
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      139 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT    188

Wildtype         212 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG    261
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      189 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG    238

Wildtype         262 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT    311
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      239 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT    288

Wildtype         312 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC    361
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      289 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC    338

Wildtype         362 GCCCTCCACACATA    375
                     ||||||||||||||
MutationRev      339 GCCCTCCACACATA    352