Is tDNA present: No

Mutation sequence:

TTCAACCAAAATCTGGTGGATTGGGGGCATTACCAGATGGAGACCGAAAAAAGAACTTCATTCCATTATTGAAAAGAAAA
AAGTGACAGAAAAGACCTGATTGGAATCGAGCCGCCCTTTATATACTCTTCCGAATCCTCCCTCTCCAAGAAGACAATCA
CCTCGAAAGTTTCTTGTTGCAACTGCTAATTCACGAAATTTAAAAATCTTGGCTCCAATTTGACCAGACTCGCCATCCTT
AACATACTCCTTTGAACCTAACAGAGTCTTTGCCTTCAACTTATCTGAACACGAAAAAAAGAGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         274 TGGTTGCCACCCTTTTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGG    323
                     |.||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TAGTTGCCACCCTTTTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGG     50

Wildtype         324 AGTAGCAGGTGTCCAGCGTGGGTTTTGAACTCCCTCAGTATCACTGTCAA    373
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTAGCAGGTGTCCAGCGTGGGTTTTGAACTCCCTCAGTATCACTGTCAA    100

Wildtype         374 ATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACT    423
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACT    150

Wildtype         424 GGACGCATGTTAGGTTGTTCCTGAACACACATTGCTGCAACTGCTAGAGC    473
                     ||||||| ||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GGACGCA-GTTAGGTTGTTCCTGAACACACATTGCTGCAACTGCTAGAGC    199

Wildtype         474 TTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTGGATCAGCCA    523
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         200 TTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTGGATCAGCCA    249

Wildtype         524 TCTGTGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGA    573
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 TCTGTGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGA    299

Wildtype         574 GAGCATGAGTTTAATATTTGAACTTGGTTCTATAAAATTGGTTTTCTAAT    623
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 GAGCATGAGTTTAATATTTGAACTTGGTTCTATAAAATTGGTTTTCTAAT    349

Wildtype         624 AGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTC    673
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 AGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTC    399

Wildtype         674 TATAGTTTCTTGGCCTTCGATTTTTCTTAATATCTATTGTCCTTGTACCA    723
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 TATAGTTTCTTGGCCTTCGATTTTTCTTAATATCTATTGTCCTTGTACCA    449

Wildtype         724 TAATTATCGAATTTGTTTTGATGTAGTTTGTGTTCTATTACTACCTGTTT    773
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         450 TAATTATCGAATTTGTTTTGATGTAGTTTGTGTTCTATTACTACCTGTTT    499

Wildtype         774 TCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGA    823
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         500 TCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGA    549

Wildtype         824 CTGCTTTGGATTGTTTTTCTTTTGAGCCAGGGGTCTATCGGAAA    867
                     ||||||||||||||||||||||||||||||||||||||||||||
Mutation         550 CTGCTTTGGATTGTTTTTCTTTTGAGCCAGGGGTCTATCGGAAA    593