Is tDNA present: Yes

Mutation sequence:

ACCCACTGAGGAATTGCAAGGATCCATAGTTTGTGCCAGAACTCTTGAGACCCCAAAATCTGCAATCTTGACCTCACGCC
TGGAGTTGATTAAGAGGTTCGAAGGTTTGATATCTCTGTGAACAATCTTACGCCTGTGGAGGTAATAGAGGCCGGATAGA
ACCTGTCGAGTTAGATCGGAGAGAGGTTGTTCGAGAGGGATATGGATCCCTTCGAGAGAGCCTTTATCCATGAACTCGAG
AAGAACTTGGATTTCTCCGTTGTGATCGAACATATCGTGACACCTAACGACGTTAGGGTTGTCTACATCTCGTAGAATCT
CGATCTCACGGCACATCTGAAGACGGACAGACTCGTGGTTACCATAGATCACTTTCAAAGCATAGAGTCTTCCAGTGGGA
CGATGTAGAACCTTGTAAACAGTACCCCCGGTGCCACTACCGATGCGATTAACCCTCTCGAGCTCAGAGAAATGTAAAGG
GGTAGGAAGCGGGGAGGAAGAGGATGAGGAAGAGGTTGGTGGAAGAGGGAGAGGAACAGCAAGAGCAACGTCACGTTGAG
GAAGAGGAAGGGTCAAATCAGTACGACGGCGGGGACGACTGCGTTGCCCGGCGGAAGATGGAGGAGGCATGGATGATGCA
GCGTTGGTGGAGTTGGCGGCTGGTCGCATGATTGATTGAATAATGGTATTGGATTGTCTGCAAAATTAATTATACAGAGA
ATGAATGAACGAACGAATGAATGAATGGGGATTTTTTTTTTAGGTTCTAAAAATCGAGAGAAAGAGAAATGCAGCCATCC
NACAC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         409 ACCCACTGAGGAATTGCAAGGATCCATAGTTTGTGCCAGAACTCTTGAGA    458
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ACCCACTGAGGAATTGCAAGGATCCATAGTTTGTGCCAGAACTCTTGAGA     50

Wildtype         459 CCCCAAAATCTGCAATCTTGACCTCACGCCTGGAGTTGATTAAGAGGTTC    508
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CCCCAAAATCTGCAATCTTGACCTCACGCCTGGAGTTGATTAAGAGGTTC    100

Wildtype         509 GAAGGTTTGATATCTCTGTGAACAATCTTACGCCTGTGGAGGTAATAGAG    558
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 GAAGGTTTGATATCTCTGTGAACAATCTTACGCCTGTGGAGGTAATAGAG    150

Wildtype         559 GCCGGATAGAACCTGTCGAGTTAGATCGGAGAGAGGTTGTTCGAGAGGGA    608
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GCCGGATAGAACCTGTCGAGTTAGATCGGAGAGAGGTTGTTCGAGAGGGA    200

Wildtype         609 TATGGATCCCTTCGAGAGAGCCTTTATCCATGAACTCGAGAAGAACTTGG    658
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TATGGATCCCTTCGAGAGAGCCTTTATCCATGAACTCGAGAAGAACTTGG    250

Wildtype         659 ATTTCTCCGTTGTGATCGAACATATCGTGACACCTAACGACGTTAGGGTT    708
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATTTCTCCGTTGTGATCGAACATATCGTGACACCTAACGACGTTAGGGTT    300

Wildtype         709 GTCTACATCTCGTAGAATCTCGATCTCACGGCACATCTGAAGACGGACAG    758
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 GTCTACATCTCGTAGAATCTCGATCTCACGGCACATCTGAAGACGGACAG    350
gRNA                                                       GAAGACGGACAG

Wildtype         759 AATCCTCGTGGTTACCATAGATCACTTTCAAAGCATAGAGTCTTCCAGTG    808
                     |   ||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 A---CTCGTGGTTACCATAGATCACTTTCAAAGCATAGAGTCTTCCAGTG    397
gRNA                 GAAGACGGACAGAATCCTCG

Wildtype         809 GGACGATGTAGAACCTTGTAAACAGTACCCCCGGTGCCACTACCGATGCG    858
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         398 GGACGATGTAGAACCTTGTAAACAGTACCCCCGGTGCCACTACCGATGCG    447

Wildtype         859 ATTAACCCTCTCGAGCTCAGAGAAATGTAAAGGGGTAGGAAGCGGGGAGG    908
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         448 ATTAACCCTCTCGAGCTCAGAGAAATGTAAAGGGGTAGGAAGCGGGGAGG    497

Wildtype         909 AAGAGGATGAGGAAGAGGTTGGTGGAAGAGGGAGAGGAACAGCAAGAGCA    958
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         498 AAGAGGATGAGGAAGAGGTTGGTGGAAGAGGGAGAGGAACAGCAAGAGCA    547

Wildtype         959 ACGTCACGTTGAGGAAGAGGAAGGGTCAAATCAGTACGACGGCGGGGACG   1008
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         548 ACGTCACGTTGAGGAAGAGGAAGGGTCAAATCAGTACGACGGCGGGGACG    597

Wildtype        1009 ACTGCGTTGCCCGGCGGAAGATGGAGGAGGCATGGATGATGCAGCGTTGG   1058
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         598 ACTGCGTTGCCCGGCGGAAGATGGAGGAGGCATGGATGATGCAGCGTTGG    647

Wildtype        1059 TGGAGTTGGCGGCTGGTCGCAT   1080
                     ||||||||||||||||||||||
Mutation         648 TGGAGTTGGCGGCTGGTCGCAT    669