Is tDNA present: Yes

Mutation sequence:

TGCAGGAGGCTTACAACGAGATGCAGACTCCCCTTGTGTATCAAACTCCTTCTGGCCTGGTAAATAGTGAGTCATTTCCA
AATGGATAGGGTTAAGATAGTTAAAATGCTTATTGTTACAGTGTTCCTTTAACAGGGATTGAAAGAGCTAAAAGAGACCA
CAGTTCAACGGGCATCGAGTACCGGGTAACATAGACGCACCAGTCGACGAGACAGTCTTTGATGTGGATAAGGATATTGA
TGAGCTCTTACCAGTAGAAGTCAAAGAACAGCGTCTAAGTAATCTCCTTCAGGCGTTAATGGTTGGTGCTTGTGTGGCTG
CTATGCCTATCTTGAAAAGGATTCCTTCTTCTGTTCTCTGGGGATACTTTGCTTTCATGGCAATAGAGAGCTTGCCAGGA
AATCAGTTTTGGGAGAGGATATTATTGCTTTTCACTGCACCAAGTAGAAGATACAAGTAAGTCACTGCCCAATGCTCCGA
TCATATCTCAGTCCCTACTCTACTTCCCATCTATTTTATTCTAATATTGTTTCGTAAGTTACAAGTGTGCCTGTCAGATC
ACATCCCAGAGAGAGGTCTAAAGTAATTAAGACTGATTAGAATCTTTAAAAAAGAGATGACTTCTTAAGTGAACTAAAAA
CTTTCTTTTCTGAAAGACCTTAAAGAGTTAGAATCAATCCTCATTTTCACCATAACCTCTTACAGTTACCCACGAGGTTC
TTACATTATACAAATA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2710 TGCAGGAGGCTTACAACGAGATGCAGACTCCCCTTGTGTATCAAACTCCT   2759
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TGCAGGAGGCTTACAACGAGATGCAGACTCCCCTTGTGTATCAAACTCCT     50

Wildtype        2760 TCTGGCCTGGTAAATAGTGAGTCATTTCCAAATGGATAGGGTTAAGATAG   2809
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TCTGGCCTGGTAAATAGTGAGTCATTTCCAAATGGATAGGGTTAAGATAG    100

Wildtype        2810 TTAAAATGCTTATTGTTACAGTGTTCCTTTAACAGGGATTGAAAGAGCTA   2859
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TTAAAATGCTTATTGTTACAGTGTTCCTTTAACAGGGATTGAAAGAGCTA    150

Wildtype        2860 AAAGAGACCACAGTTCAACGGGCATCGAGTACCGGGT-ACATAGACGCAC   2908
                     ||||||||||||||||||||||||||||||||||||| ||||||||||||
Mutation         151 AAAGAGACCACAGTTCAACGGGCATCGAGTACCGGGTAACATAGACGCAC    200
gRNA                                                   GGT-ACATAGACGCAC

Wildtype        2909 CAGTCGACGAGACAGTCTTTGATGTGGATAAGGATATTGATGAGCTCTTA   2958
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CAGTCGACGAGACAGTCTTTGATGTGGATAAGGATATTGATGAGCTCTTA    250
gRNA                 GGTACATAGACGCACCAGTC

Wildtype        2959 CCAGTAGAAGTCAAAGAACAGCGTCTAAGTAATCTCCTTCAGGCGTTAAT   3008
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CCAGTAGAAGTCAAAGAACAGCGTCTAAGTAATCTCCTTCAGGCGTTAAT    300

Wildtype        3009 GGTTGGTGCTTGTGTGGCTGCTATGCCTATCTTGAAAAGGATTCCTTCTT   3058
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 GGTTGGTGCTTGTGTGGCTGCTATGCCTATCTTGAAAAGGATTCCTTCTT    350

Wildtype        3059 CTGTTCTCTGGGGATACTTTGCTTTCATGGCAATAGAGAGCTTGCCAGGA   3108
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CTGTTCTCTGGGGATACTTTGCTTTCATGGCAATAGAGAGCTTGCCAGGA    400

Wildtype        3109 AATCAGTTTTGGGAGAGGATATTATTGCTTTTCACTGCACCAAGTAGAAG   3158
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 AATCAGTTTTGGGAGAGGATATTATTGCTTTTCACTGCACCAAGTAGAAG    450

Wildtype        3159 ATACAAGTAAGTCACTGCCCAATGCTCCGATCATATCTCAGTCCCTACTC   3208
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 ATACAAGTAAGTCACTGCCCAATGCTCCGATCATATCTCAGTCCCTACTC    500

Wildtype        3209 TACTTCCCATCTATTTTATTCTAATATTGTTTCGTAAGTTACAAGTGTGC   3258
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TACTTCCCATCTATTTTATTCTAATATTGTTTCGTAAGTTACAAGTGTGC    550

Wildtype        3259 CTGTCAGATCACATCCCAGAGAGAGGTCTAAAGTAATTAAGACTGATTAG   3308
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 CTGTCAGATCACATCCCAGAGAGAGGTCTAAAGTAATTAAGACTGATTAG    600

Wildtype        3309 AATCTTTAAAAAAGAGATGACTTCTTAAGTGAACTAAAAACTTTCTTTTC   3358
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 AATCTTTAAAAAAGAGATGACTTCTTAAGTGAACTAAAAACTTTCTTTTC    650

Wildtype        3359 TGAAAGACCTTAAAGAGTTAGAATCAATCCTCATTTTCACCATAACCTCT   3408
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TGAAAGACCTTAAAGAGTTAGAATCAATCCTCATTTTCACCATAACCTCT    700

Wildtype        3409 TACAGTTACCCACGAGGTTCTTACATTATACAAATA   3444
                     ||||||||||||||||||||||||||||||||||||
Mutation         701 TACAGTTACCCACGAGGTTCTTACATTATACAAATA    736