Is tDNA present:

Mutation sequence:

ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAGGCAAGTCACTTTTGCTAAGAGAAGAAATGG
ACTTCTCAAGAAAGCTTATGAACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAATCGTGGTAAAC
TCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAATTTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCC
TACTTTAACCAAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACTTCTGTGTTCTCTCTCTCTCT
CACTTCAATTATAATGCAAGATCTGAATTGTCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT
CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTGTAAGATCTACTTATTTATATTCTATTTAAA
CTTTTGCGCATATAAAAAAAATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTGAATGTTTTCT
TAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTTAACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAG
ATCATCTGTGGAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGTCATATCTTTTCTTGATTTTA
ATTTTGGTTAATAAAGAAAATGTAACTTTATAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT
AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAACTTGCAATTTTACACTTTATAATTTTTTTT
ACTGTTAAGATTTGATTTGCAGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCTTTGCTTCCCA
AGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAACCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATT
CTTACTAGTGATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATTTGTGACCCGGAGTCTGTATA
TATTGGTAATTCAACAAAAATAACAAATTCAGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT
AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATTTTCGGCTCAAGTGAATTATGTAAAATATAG
TTTATATATTTTTACCCAATCTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTCCCAAGAGTGT
ATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAAATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATT
TTTAATAATTAAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAAAGAGCTTAGAATAATTTTCA
TTATATCCAGAGAGAACTTTGAAGTTTTTCTTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA
CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGTTGTAGAGTCACATGAAATAATTACTACAAC
TTTCATCTTATTGTTGCAGCATGGTGAAAACAATTGAAAAGTATCAACGTTCTGGAAGCCAACCAATCAGCTACTGATAC
TCAGGTACTCTAACATTAAATTTGTTTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCTTTTAT
TTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACATTTTGAATATCCTACAAATCTTTAGTTTGAGACCA
TAAGATTTCAAATACCTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTATTAAAACGGAGGGAG
AATATATTATGTATTCGTGTACTCTTTGATCTATTCATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAA
ACTTTTGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTTAAAATTAGGGTTGTGTTTGAACATG
AACTTAAACTGGAGTTGTTTTAAACTTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCAAGTTT
ATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAATATCCTGGCCAAACGGATTAGTCTCTATATCTAGT
AGCGGACCTGCATTGAGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTATTTATATAGAAGTTT
AAGGAAATCGTATAATTTTTAACATAGAAAAAGTGGATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAA
ATCCTAGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCTTTCGTGTCATTAACTTAATTAATCA
TATTTATTTTCTTCATAACTAATTAGTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTATGTTGA
AATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTTTTCTTATGTTTAGCAAAAGCTATTTTAATATCTTT
GAAGCATATTCACTCAAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTTATCTGATGTCCAGTT
GGAACCCTGACTAAATTTATTTACCAATTCATTCTGACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAAC
GAAATGATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATCCTTAACAAATATTTTCTTCATACCA
AATGATTGAATTTCAGGCCTTCAAATATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCAGTAAT
TATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTTATTGTCATATCACCTATAAAGTCATCACCTAGTCA
GATGATATATTTGGTGAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAAAACTTTTCATTTTCA
ACTCATCATTTGATTTAGCAGGTGGAGCAGATCATTCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGA
AGTTTTCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTTGGAAAATGGCCATAAATGAGTTATT
TTTTATGTAAATAATCAGCATTTTAGTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACTAATGT
TACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAAAGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGG
TATGTTTTGCTCACTATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTATTTTCGAAAATCATTC
TATATATTCTATTTTTTGATGTATAATTAACTTGTTTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTA
TTGATACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCATTAAACACAAAGGACCTTGAGCAGC
TTGAGAATCAATTAGAGTCGTCCTTGAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGATAAAAC
ACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTATATGAATGATTTTAATTCCATTTTAAATGCTATATA
ATTGTTTTATCTTGATTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGTATTGCTCGCGTAGTA
TACACGTTCCATGTCCAATTTTAAGAATATGGATTCAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATAT
TAAATTAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTGTGTTAAAAAGGTGCCATCTAAACTT
TAAGGCAAGTTATACGTAGTGATGGTTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCACATTCA
AAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTGAGGATACTAGGTGGAATAAAATGGAAAATAAGAAT
ATCCAAGAAAAGGTGAGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATTGGGCATGTGATGAGA
AGATGCATAGATGACCTAGTGCAAAGGTGTAAGAAGTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAA
GTATAGCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTACATGACCTCATATAAGAAAGTGTGGA
GGATGAACTAGGACAGAAGAATAGTAAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCCCTCAC
TAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTTTGTTGATATCTATTATTCATGTAGTTTGATTCTAG
CTAGTATATATTTAGTTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAGGTCATCCAAAAAAAA
TTATGAGAACTCTTCTTCAAATATAAAATTTGAAATCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAG
ATGAATAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAAGGTTTTTTTTAAAAAAAATTTAAGA
TATTTTCTTTGGTTTTTGTTACTCTTATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATCTTTTT
GATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATGAATTTTTAAATTTGCAGATTAATTGACATTAAATT
TATAAAAGAAAAAAGTAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAAATCTTTCATAACAAA
TGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTAAGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCA
AAGAAGCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGAGCGTCAGTGATACTGCGATTAGAAG
GTAAACAAAAGTTGTACTAGAAATATTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGATTTTT
TTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTATGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGA
AACAACAAAATGCAGTAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGGCAATGGGGGATGCAT
TGGATATAGGCAAATCGAAAACGTATTAAACAAAAATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATT
AATTTATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATACTAGCTAAAATTAAAGATCAAAATAT
GCTTAGCAAAAACTTATGGAAAATAACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCCTAAGT
GAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTAAAGGGTCGAATAATATATAGTATGGTTACTTATTT
TGCCAACCCTACCAACAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGATCAGCTTGCACAACTT
CAACAAAGGGTACTCATTAGAATTTAAATTTTTTCAAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATT
TCAAATTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAGATTACTCCGCAGAAAGGTAAAACAT
TAATGACCAACCAATTCAAAGATTATATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTATAACT
AGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGTAACTTGATAATGTACAGAGTTTTAATACTGAATTG
AAATTGGTGTTGTGTGTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGCTGGGAAGATAATGGA
GCTGATCAAGCCATGAATCAACACAATCGTCTCCCTCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCC
CCCACAATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTTTATTTCTATTAATAGATGATCAATA
TGCACCAATAAGTTGTTAAATAGATAGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATATGCAAT
GTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTGACCCAGATTGAACTCTTAATAAAAAGGCCAATGTC
CAACATTAAACAATTCTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTTTTGCATAACAAAAAT
AGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAATTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAA
TATATAGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAATATTTGTTGTAAATGTAGTTCCTAGC
AAATAATGGAGGATTGCTGATAGAAACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTATGTTAA
AAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCTATGTTTATATAAGAAAAATATATATAAAAATTGGT
ATAAGATGGAAAAAAAGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAAACCTCACAGCTTTGT
GTGTCATATGTTCAACCTTATTAGCACCCTAACCCCTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTC
GAGCGCTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTACATTATGCAATTCAGATTTAGTCGA
GAGCGATATAGATGGACTATACATGTCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTGCTCCT
TGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAGGGAGGACCTGAATTCACATAAATTTGGATTAAAAA
TTAGGTTTTCTTATACAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTGATTGTTGTTCTGATG
AATTATTTCTAGGTACAATCCTGTTAATACAGATGAGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTC
ATGGGTGGATGCTTTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50

Wildtype          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100

Wildtype         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150
gRNA                                                 CTTATCATCTTCTCTAAT

Wildtype         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200
gRNA                 CTTATCATCTTCTCTAATCG

Wildtype         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250

Wildtype         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300

Wildtype         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350

Wildtype         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400

Wildtype         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450

Wildtype         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500

Wildtype         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550

Wildtype         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600

Wildtype         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650

Wildtype         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700

Wildtype         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750

Wildtype         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800

Wildtype         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850

Wildtype         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900

Wildtype         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950

Wildtype         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000

Wildtype        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050

Wildtype        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100

Wildtype        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150

Wildtype        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200

Wildtype        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250

Wildtype        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300

Wildtype        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350

Wildtype        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400

Wildtype        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450

Wildtype        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500

Wildtype        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550

Wildtype        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600

Wildtype        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650

Wildtype        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700

Wildtype        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCT-ACTCTGG   1749
                     |||||||||||||||||||||||||||||||||||||||||| |||||||
Mutation        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCTAACTCTGG   1750

Wildtype        1750 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1800

Wildtype        1800 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1850

Wildtype        1850 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1900

Wildtype        1900 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1950

Wildtype        1950 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   2000

Wildtype        2000 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2050

Wildtype        2050 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2100

Wildtype        2100 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2150

Wildtype        2150 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2151 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2200

Wildtype        2200 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2201 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2250

Wildtype        2250 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2251 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2300

Wildtype        2300 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2301 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2350

Wildtype        2350 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2351 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2400

Wildtype        2400 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2401 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2450

Wildtype        2450 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2451 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2500

Wildtype        2500 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2501 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2550

Wildtype        2550 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2551 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2600

Wildtype        2600 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2601 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2650

Wildtype        2650 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2651 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2700

Wildtype        2700 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2701 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2750

Wildtype        2750 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2751 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2800

Wildtype        2800 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2801 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2850

Wildtype        2850 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2851 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2900

Wildtype        2900 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2901 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2950

Wildtype        2950 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   2999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2951 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   3000

Wildtype        3000 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3001 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3050

Wildtype        3050 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3051 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3100

Wildtype        3100 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3101 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3150

Wildtype        3150 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3151 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3200

Wildtype        3200 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3201 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3250

Wildtype        3250 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3251 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3300

Wildtype        3300 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3301 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3350

Wildtype        3350 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3351 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3400

Wildtype        3400 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3401 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3450

Wildtype        3450 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3451 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3500

Wildtype        3500 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3501 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3550

Wildtype        3550 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3551 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3600

Wildtype        3600 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3601 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3650

Wildtype        3650 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3651 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3700

Wildtype        3700 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3701 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3750

Wildtype        3750 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3751 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3800

Wildtype        3800 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3801 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3850

Wildtype        3850 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3851 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3900

Wildtype        3900 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3901 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3950

Wildtype        3950 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   3999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3951 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   4000

Wildtype        4000 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4001 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4050

Wildtype        4050 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4051 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4100

Wildtype        4100 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4101 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4150

Wildtype        4150 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4151 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4200

Wildtype        4200 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4201 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4250

Wildtype        4250 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4251 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4300

Wildtype        4300 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4301 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4350

Wildtype        4350 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4351 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4400

Wildtype        4400 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4401 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4450

Wildtype        4450 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4451 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4500

Wildtype        4500 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4501 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4550

Wildtype        4550 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4551 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4600

Wildtype        4600 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4601 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4650

Wildtype        4650 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4651 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4700

Wildtype        4700 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4701 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4750

Wildtype        4750 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4751 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4800

Wildtype        4800 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4801 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4850

Wildtype        4850 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4851 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4900

Wildtype        4900 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4901 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4950

Wildtype        4950 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   4999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4951 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   5000

Wildtype        5000 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5001 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5050

Wildtype        5050 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5051 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5100

Wildtype        5100 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5101 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5150

Wildtype        5150 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5151 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5200

Wildtype        5200 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5201 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5250

Wildtype        5250 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5251 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5300

Wildtype        5300 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5301 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5350

Wildtype        5350 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5351 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5400

Wildtype        5400 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5401 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5450

Wildtype        5450 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5451 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5500

Wildtype        5500 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5501 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5550

Wildtype        5550 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5551 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5600

Wildtype        5600 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5601 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5650

Wildtype        5650 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5651 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5700

Wildtype        5700 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5701 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5750

Wildtype        5750 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5751 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5800

Wildtype        5800 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5801 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5850

Wildtype        5850 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5851 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5900

Wildtype        5900 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5901 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5950

Wildtype        5950 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   5999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5951 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   6000

Wildtype        6000 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6001 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6050

Wildtype        6050 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6051 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6100

Wildtype        6100 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6101 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6150

Wildtype        6150 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6151 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6200

Wildtype        6200 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6201 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6250

Wildtype        6250 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6251 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6300

Wildtype        6300 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6301 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6350

Wildtype        6350 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6351 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6400

Wildtype        6400 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6401 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6450

Wildtype        6450 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6451 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6500

Wildtype        6500 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6501 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6550

Wildtype        6550 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6551 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6600

Wildtype        6600 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6601 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6650

Wildtype        6650 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6651 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6700

Wildtype        6700 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6701 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6750

Wildtype        6750 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6751 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6800

Wildtype        6800 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6801 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6850

Wildtype        6850 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6851 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6900

Wildtype        6900 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6901 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6950

Wildtype        6950 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   6999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6951 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   7000

Wildtype        7000 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7001 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7050

Wildtype        7050 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7051 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7100

Wildtype        7100 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7101 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7150

Wildtype        7150 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7151 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7200

Wildtype        7200 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7201 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7250

Wildtype        7250 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7251 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7300

Wildtype        7300 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7301 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7350

Wildtype        7350 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7351 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7400

Wildtype        7400 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7401 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7450

Wildtype        7450 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7451 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7500

Wildtype        7500 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7501 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7550

Wildtype        7550 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7551 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7600

Wildtype        7600 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7601 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7650

Wildtype        7650 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7651 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7700

Wildtype        7700 TGGATGCTTTAA   7711
                     ||||||||||||
Mutation        7701 TGGATGCTTTAA   7712

Is tDNA present:

Mutation sequence:

ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAGGCAAGTCACTTTTGCTAAGAGAAGAAATGG
ACTTCTCAAGAAAGCTTATGAACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAATCGTGGTAAAC
TCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAATTTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCC
TACTTTAACCAAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACTTCTGTGTTCTCTCTCTCTCT
CACTTCAATTATAATGCAAGATCTGAATTGTCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT
CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTGTAAGATCTACTTATTTATATTCTATTTAAA
CTTTTGCGCATATAAAAAAAATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTGAATGTTTTCT
TAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTTAACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAG
ATCATCTGTGGAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGTCATATCTTTTCTTGATTTTA
ATTTTGGTTAATAAAGAAAATGTAACTTTATAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT
AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAACTTGCAATTTTACACTTTATAATTTTTTTT
ACTGTTAAGATTTGATTTGCAGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCTTTGCTTCCCA
AGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAACCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATT
CTTACTAGTGATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATTTGTGACCCGGAGTCTGTATA
TATTGGTAATTCAACAAAAATAACAAATTCAGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT
AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATTTTCGGCTCAAGTGAATTATGTAAAATATAG
TTTATATATTTTTACCCAATCTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTCCCAAGAGTGT
ATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAAATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATT
TTTAATAATTAAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAAAGAGCTTAGAATAATTTTCA
TTATATCCAGAGAGAACTTTGAAGTTTTTCTTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA
CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGTTGTAGAGTCACATGAAATAATTACTACAAC
TTTCATCTTATTGTTGCAGCATGGTGAAAACAATTGAAAAGTATCAACGTTCTGGAAGCCAACCAATCAGCTACTGATAC
TCAGGTACTCTAACATTAAATTTGTTTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCTTTTAT
TTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACATTTTGAATATCCTACAAATCTTTAGTTTGAGACCA
TAAGATTTCAAATACCTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTATTAAAACGGAGGGAG
AATATATTATGTATTCGTGTACTCTTTGATCTATTCATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAA
ACTTTTGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTTAAAATTAGGGTTGTGTTTGAACATG
AACTTAAACTGGAGTTGTTTTAAACTTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCAAGTTT
ATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAATATCCTGGCCAAACGGATTAGTCTCTATATCTAGT
AGCGGACCTGCATTGAGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTATTTATATAGAAGTTT
AAGGAAATCGTATAATTTTTAACATAGAAAAAGTGGATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAA
ATCCTAGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCTTTCGTGTCATTAACTTAATTAATCA
TATTTATTTTCTTCATAACTAATTAGTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTATGTTGA
AATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTTTTCTTATGTTTAGCAAAAGCTATTTTAATATCTTT
GAAGCATATTCACTCAAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTTATCTGATGTCCAGTT
GGAACCCTGACTAAATTTATTTACCAATTCATTCTGACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAAC
GAAATGATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATCCTTAACAAATATTTTCTTCATACCA
AATGATTGAATTTCAGGCCTTCAAATATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCAGTAAT
TATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTTATTGTCATATCACCTATAAAGTCATCACCTAGTCA
GATGATATATTTGGTGAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAAAACTTTTCATTTTCA
ACTCATCATTTGATTTAGCAGGTGGAGCAGATCATTCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGA
AGTTTTCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTTGGAAAATGGCCATAAATGAGTTATT
TTTTATGTAAATAATCAGCATTTTAGTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACTAATGT
TACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAAAGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGG
TATGTTTTGCTCACTATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTATTTTCGAAAATCATTC
TATATATTCTATTTTTTGATGTATAATTAACTTGTTTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTA
TTGATACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCATTAAACACAAAGGACCTTGAGCAGC
TTGAGAATCAATTAGAGTCGTCCTTGAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGATAAAAC
ACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTATATGAATGATTTTAATTCCATTTTAAATGCTATATA
ATTGTTTTATCTTGATTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGTATTGCTCGCGTAGTA
TACACGTTCCATGTCCAATTTTAAGAATATGGATTCAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATAT
TAAATTAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTGTGTTAAAAAGGTGCCATCTAAACTT
TAAGGCAAGTTATACGTAGTGATGGTTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCACATTCA
AAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTGAGGATACTAGGTGGAATAAAATGGAAAATAAGAAT
ATCCAAGAAAAGGTGAGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATTGGGCATGTGATGAGA
AGATGCATAGATGACCTAGTGCAAAGGTGTAAGAAGTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAA
GTATAGCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTACATGACCTCATATAAGAAAGTGTGGA
GGATGAACTAGGACAGAAGAATAGTAAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCCCTCAC
TAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTTTGTTGATATCTATTATTCATGTAGTTTGATTCTAG
CTAGTATATATTTAGTTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAGGTCATCCAAAAAAAA
TTATGAGAACTCTTCTTCAAATATAAAATTTGAAATCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAG
ATGAATAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAAGGTTTTTTTTAAAAAAAATTTAAGA
TATTTTCTTTGGTTTTTGTTACTCTTATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATCTTTTT
GATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATGAATTTTTAAATTTGCAGATTAATTGACATTAAATT
TATAAAAGAAAAAAGTAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAAATCTTTCATAACAAA
TGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTAAGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCA
AAGAAGCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGAGCGTCAGTGATACTGCGATTAGAAG
GTAAACAAAAGTTGTACTAGAAATATTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGATTTTT
TTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTATGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGA
AACAACAAAATGCAGTAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGGCAATGGGGGATGCAT
TGGATATAGGCAAATCGAAAACGTATTAAACAAAAATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATT
AATTTATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATACTAGCTAAAATTAAAGATCAAAATAT
GCTTAGCAAAAACTTATGGAAAATAACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCCTAAGT
GAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTAAAGGGTCGAATAATATATAGTATGGTTACTTATTT
TGCCAACCCTACCAACAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGATCAGCTTGCACAACTT
CAACAAAGGGTACTCATTAGAATTTAAATTTTTTCAAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATT
TCAAATTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAGATTACTCCGCAGAAAGGTAAAACAT
TAATGACCAACCAATTCAAAGATTATATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTATAACT
AGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGTAACTTGATAATGTACAGAGTTTTAATACTGAATTG
AAATTGGTGTTGTGTGTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGCTGGGAAGATAATGGA
GCTGATCAAGCCATGAATCAACACAATCGTCTCCCTCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCC
CCCACAATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTTTATTTCTATTAATAGATGATCAATA
TGCACCAATAAGTTGTTAAATAGATAGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATATGCAAT
GTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTGACCCAGATTGAACTCTTAATAAAAAGGCCAATGTC
CAACATTAAACAATTCTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTTTTGCATAACAAAAAT
AGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAATTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAA
TATATAGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAATATTTGTTGTAAATGTAGTTCCTAGC
AAATAATGGAGGATTGCTGATAGAAACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTATGTTAA
AAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCTATGTTTATATAAGAAAAATATATATAAAAATTGGT
ATAAGATGGAAAAAAAGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAAACCTCACAGCTTTGT
GTGTCATATGTTCAACCTTATTAGCACCCTAACCCCTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTC
GAGCGCTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTACATTATGCAATTCAGATTTAGTCGA
GAGCGATATAGATGGACTATACATGTCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTGCTCCT
TGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAGGGAGGACCTGAATTCACATAAATTTGGATTAAAAA
TTAGGTTTTCTTATACAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTGATTGTTGTTCTGATG
AATTATTTCTAGGTACAATCCTGTTAATACAGATGAGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTC
ATGGGTGGATGCTTTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50

Wildtype          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100

Wildtype         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150
gRNA                                                 CTTATCATCTTCTCTAAT

Wildtype         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200
gRNA                 CTTATCATCTTCTCTAATCG

Wildtype         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250

Wildtype         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300

Wildtype         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350

Wildtype         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400

Wildtype         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450

Wildtype         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500

Wildtype         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550

Wildtype         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600

Wildtype         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650

Wildtype         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700

Wildtype         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750

Wildtype         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800

Wildtype         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850

Wildtype         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900

Wildtype         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950

Wildtype         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000

Wildtype        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050

Wildtype        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100

Wildtype        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150

Wildtype        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200

Wildtype        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250

Wildtype        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300

Wildtype        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350

Wildtype        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400

Wildtype        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450

Wildtype        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500

Wildtype        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550

Wildtype        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600

Wildtype        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650

Wildtype        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700

Wildtype        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCT-ACTCTGG   1749
                     |||||||||||||||||||||||||||||||||||||||||| |||||||
Mutation        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCTAACTCTGG   1750

Wildtype        1750 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1800

Wildtype        1800 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1850

Wildtype        1850 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1900

Wildtype        1900 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1950

Wildtype        1950 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   2000

Wildtype        2000 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2050

Wildtype        2050 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2100

Wildtype        2100 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2150

Wildtype        2150 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2151 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2200

Wildtype        2200 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2201 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2250

Wildtype        2250 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2251 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2300

Wildtype        2300 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2301 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2350

Wildtype        2350 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2351 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2400

Wildtype        2400 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2401 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2450

Wildtype        2450 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2451 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2500

Wildtype        2500 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2501 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2550

Wildtype        2550 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2551 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2600

Wildtype        2600 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2601 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2650

Wildtype        2650 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2651 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2700

Wildtype        2700 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2701 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2750

Wildtype        2750 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2751 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2800

Wildtype        2800 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2801 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2850

Wildtype        2850 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2851 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2900

Wildtype        2900 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2901 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2950

Wildtype        2950 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   2999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2951 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   3000

Wildtype        3000 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3001 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3050

Wildtype        3050 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3051 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3100

Wildtype        3100 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3101 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3150

Wildtype        3150 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3151 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3200

Wildtype        3200 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3201 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3250

Wildtype        3250 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3251 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3300

Wildtype        3300 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3301 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3350

Wildtype        3350 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3351 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3400

Wildtype        3400 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3401 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3450

Wildtype        3450 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3451 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3500

Wildtype        3500 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3501 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3550

Wildtype        3550 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3551 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3600

Wildtype        3600 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3601 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3650

Wildtype        3650 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3651 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3700

Wildtype        3700 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3701 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3750

Wildtype        3750 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3751 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3800

Wildtype        3800 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3801 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3850

Wildtype        3850 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3851 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3900

Wildtype        3900 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3901 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3950

Wildtype        3950 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   3999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3951 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   4000

Wildtype        4000 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4001 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4050

Wildtype        4050 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4051 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4100

Wildtype        4100 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4101 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4150

Wildtype        4150 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4151 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4200

Wildtype        4200 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4201 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4250

Wildtype        4250 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4251 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4300

Wildtype        4300 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4301 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4350

Wildtype        4350 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4351 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4400

Wildtype        4400 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4401 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4450

Wildtype        4450 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4451 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4500

Wildtype        4500 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4501 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4550

Wildtype        4550 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4551 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4600

Wildtype        4600 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4601 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4650

Wildtype        4650 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4651 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4700

Wildtype        4700 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4701 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4750

Wildtype        4750 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4751 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4800

Wildtype        4800 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4801 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4850

Wildtype        4850 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4851 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4900

Wildtype        4900 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4901 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4950

Wildtype        4950 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   4999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4951 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   5000

Wildtype        5000 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5001 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5050

Wildtype        5050 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5051 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5100

Wildtype        5100 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5101 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5150

Wildtype        5150 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5151 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5200

Wildtype        5200 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5201 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5250

Wildtype        5250 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5251 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5300

Wildtype        5300 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5301 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5350

Wildtype        5350 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5351 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5400

Wildtype        5400 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5401 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5450

Wildtype        5450 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5451 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5500

Wildtype        5500 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5501 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5550

Wildtype        5550 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5551 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5600

Wildtype        5600 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5601 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5650

Wildtype        5650 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5651 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5700

Wildtype        5700 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5701 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5750

Wildtype        5750 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5751 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5800

Wildtype        5800 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5801 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5850

Wildtype        5850 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5851 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5900

Wildtype        5900 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5901 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5950

Wildtype        5950 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   5999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5951 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   6000

Wildtype        6000 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6001 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6050

Wildtype        6050 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6051 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6100

Wildtype        6100 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6101 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6150

Wildtype        6150 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6151 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6200

Wildtype        6200 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6201 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6250

Wildtype        6250 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6251 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6300

Wildtype        6300 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6301 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6350

Wildtype        6350 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6351 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6400

Wildtype        6400 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6401 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6450

Wildtype        6450 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6451 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6500

Wildtype        6500 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6501 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6550

Wildtype        6550 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6551 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6600

Wildtype        6600 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6601 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6650

Wildtype        6650 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6651 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6700

Wildtype        6700 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6701 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6750

Wildtype        6750 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6751 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6800

Wildtype        6800 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6801 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6850

Wildtype        6850 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6851 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6900

Wildtype        6900 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6901 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6950

Wildtype        6950 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   6999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6951 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   7000

Wildtype        7000 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7001 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7050

Wildtype        7050 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7051 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7100

Wildtype        7100 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7101 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7150

Wildtype        7150 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7151 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7200

Wildtype        7200 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7201 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7250

Wildtype        7250 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7251 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7300

Wildtype        7300 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7301 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7350

Wildtype        7350 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7351 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7400

Wildtype        7400 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7401 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7450

Wildtype        7450 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7451 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7500

Wildtype        7500 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7501 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7550

Wildtype        7550 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7551 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7600

Wildtype        7600 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7601 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7650

Wildtype        7650 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7651 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7700

Wildtype        7700 TGGATGCTTTAA   7711
                     ||||||||||||
Mutation        7701 TGGATGCTTTAA   7712

Is tDNA present:

Mutation sequence:

ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAGGCAAGTCACTTTTGCTAAGAGAAGAAATGG
ACTTCTCAAGAAAGCTTATGAACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAATCGTGGTAAAC
TCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAATTTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCC
TACTTTAACCAAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACTTCTGTGTTCTCTCTCTCTCT
CACTTCAATTATAATGCAAGATCTGAATTGTCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT
CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTGTAAGATCTACTTATTTATATTCTATTTAAA
CTTTTGCGCATATAAAAAAAATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTGAATGTTTTCT
TAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTTAACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAG
ATCATCTGTGGAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGTCATATCTTTTCTTGATTTTA
ATTTTGGTTAATAAAGAAAATGTAACTTTATAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT
AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAACTTGCAATTTTACACTTTATAATTTTTTTT
ACTGTTAAGATTTGATTTGCAGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCTTTGCTTCCCA
AGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAACCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATT
CTTACTAGTGATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATTTGTGACCCGGAGTCTGTATA
TATTGGTAATTCAACAAAAATAACAAATTCAGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT
AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATTTTCGGCTCAAGTGAATTATGTAAAATATAG
TTTATATATTTTTACCCAATCTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTCCCAAGAGTGT
ATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAAATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATT
TTTAATAATTAAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAAAGAGCTTAGAATAATTTTCA
TTATATCCAGAGAGAACTTTGAAGTTTTTCTTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA
CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGTTGTAGAGTCACATGAAATAATTACTACAAC
TTTCATCTTATTGTTGCAGCATGGTGAAAACAATTGAAAAGTATCAACGTTCTGGAAGCCAACCAATCAGCTACTGATAC
TCAGGTACTCTAACATTAAATTTGTTTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCTTTTAT
TTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACATTTTGAATATCCTACAAATCTTTAGTTTGAGACCA
TAAGATTTCAAATACCTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTATTAAAACGGAGGGAG
AATATATTATGTATTCGTGTACTCTTTGATCTATTCATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAA
ACTTTTGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTTAAAATTAGGGTTGTGTTTGAACATG
AACTTAAACTGGAGTTGTTTTAAACTTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCAAGTTT
ATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAATATCCTGGCCAAACGGATTAGTCTCTATATCTAGT
AGCGGACCTGCATTGAGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTATTTATATAGAAGTTT
AAGGAAATCGTATAATTTTTAACATAGAAAAAGTGGATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAA
ATCCTAGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCTTTCGTGTCATTAACTTAATTAATCA
TATTTATTTTCTTCATAACTAATTAGTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTATGTTGA
AATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTTTTCTTATGTTTAGCAAAAGCTATTTTAATATCTTT
GAAGCATATTCACTCAAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTTATCTGATGTCCAGTT
GGAACCCTGACTAAATTTATTTACCAATTCATTCTGACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAAC
GAAATGATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATCCTTAACAAATATTTTCTTCATACCA
AATGATTGAATTTCAGGCCTTCAAATATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCAGTAAT
TATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTTATTGTCATATCACCTATAAAGTCATCACCTAGTCA
GATGATATATTTGGTGAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAAAACTTTTCATTTTCA
ACTCATCATTTGATTTAGCAGGTGGAGCAGATCATTCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGA
AGTTTTCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTTGGAAAATGGCCATAAATGAGTTATT
TTTTATGTAAATAATCAGCATTTTAGTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACTAATGT
TACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAAAGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGG
TATGTTTTGCTCACTATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTATTTTCGAAAATCATTC
TATATATTCTATTTTTTGATGTATAATTAACTTGTTTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTA
TTGATACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCATTAAACACAAAGGACCTTGAGCAGC
TTGAGAATCAATTAGAGTCGTCCTTGAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGATAAAAC
ACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTATATGAATGATTTTAATTCCATTTTAAATGCTATATA
ATTGTTTTATCTTGATTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGTATTGCTCGCGTAGTA
TACACGTTCCATGTCCAATTTTAAGAATATGGATTCAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATAT
TAAATTAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTGTGTTAAAAAGGTGCCATCTAAACTT
TAAGGCAAGTTATACGTAGTGATGGTTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCACATTCA
AAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTGAGGATACTAGGTGGAATAAAATGGAAAATAAGAAT
ATCCAAGAAAAGGTGAGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATTGGGCATGTGATGAGA
AGATGCATAGATGACCTAGTGCAAAGGTGTAAGAAGTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAA
GTATAGCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTACATGACCTCATATAAGAAAGTGTGGA
GGATGAACTAGGACAGAAGAATAGTAAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCCCTCAC
TAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTTTGTTGATATCTATTATTCATGTAGTTTGATTCTAG
CTAGTATATATTTAGTTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAGGTCATCCAAAAAAAA
TTATGAGAACTCTTCTTCAAATATAAAATTTGAAATCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAG
ATGAATAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAAGGTTTTTTTTAAAAAAAATTTAAGA
TATTTTCTTTGGTTTTTGTTACTCTTATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATCTTTTT
GATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATGAATTTTTAAATTTGCAGATTAATTGACATTAAATT
TATAAAAGAAAAAAGTAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAAATCTTTCATAACAAA
TGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTAAGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCA
AAGAAGCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGAGCGTCAGTGATACTGCGATTAGAAG
GTAAACAAAAGTTGTACTAGAAATATTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGATTTTT
TTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTATGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGA
AACAACAAAATGCAGTAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGGCAATGGGGGATGCAT
TGGATATAGGCAAATCGAAAACGTATTAAACAAAAATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATT
AATTTATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATACTAGCTAAAATTAAAGATCAAAATAT
GCTTAGCAAAAACTTATGGAAAATAACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCCTAAGT
GAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTAAAGGGTCGAATAATATATAGTATGGTTACTTATTT
TGCCAACCCTACCAACAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGATCAGCTTGCACAACTT
CAACAAAGGGTACTCATTAGAATTTAAATTTTTTCAAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATT
TCAAATTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAGATTACTCCGCAGAAAGGTAAAACAT
TAATGACCAACCAATTCAAAGATTATATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTATAACT
AGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGTAACTTGATAATGTACAGAGTTTTAATACTGAATTG
AAATTGGTGTTGTGTGTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGCTGGGAAGATAATGGA
GCTGATCAAGCCATGAATCAACACAATCGTCTCCCTCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCC
CCCACAATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTTTATTTCTATTAATAGATGATCAATA
TGCACCAATAAGTTGTTAAATAGATAGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATATGCAAT
GTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTGACCCAGATTGAACTCTTAATAAAAAGGCCAATGTC
CAACATTAAACAATTCTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTTTTGCATAACAAAAAT
AGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAATTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAA
TATATAGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAATATTTGTTGTAAATGTAGTTCCTAGC
AAATAATGGAGGATTGCTGATAGAAACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTATGTTAA
AAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCTATGTTTATATAAGAAAAATATATATAAAAATTGGT
ATAAGATGGAAAAAAAGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAAACCTCACAGCTTTGT
GTGTCATATGTTCAACCTTATTAGCACCCTAACCCCTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTC
GAGCGCTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTACATTATGCAATTCAGATTTAGTCGA
GAGCGATATAGATGGACTATACATGTCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTGCTCCT
TGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAGGGAGGACCTGAATTCACATAAATTTGGATTAAAAA
TTAGGTTTTCTTATACAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTGATTGTTGTTCTGATG
AATTATTTCTAGGTACAATCCTGTTAATACAGATGAGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTC
ATGGGTGGATGCTTTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50

Wildtype          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100

Wildtype         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150

Wildtype         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200

Wildtype         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250

Wildtype         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300

Wildtype         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350

Wildtype         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400

Wildtype         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450

Wildtype         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500

Wildtype         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550

Wildtype         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600

Wildtype         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650

Wildtype         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700

Wildtype         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750

Wildtype         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800

Wildtype         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850

Wildtype         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900

Wildtype         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950

Wildtype         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000

Wildtype        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050

Wildtype        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100

Wildtype        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150

Wildtype        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200

Wildtype        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250

Wildtype        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300

Wildtype        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350

Wildtype        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400

Wildtype        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450

Wildtype        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500

Wildtype        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550

Wildtype        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600

Wildtype        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650

Wildtype        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700

Wildtype        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCT-ACTCTGG   1749
                     |||||||||||||||||||||||||||||||||||||||||| |||||||
Mutation        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCTAACTCTGG   1750
gRNA                                           ACGTTGCAGCTATGCT-ACTC

Wildtype        1750 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1800

Wildtype        1800 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1850

Wildtype        1850 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1900

Wildtype        1900 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1950

Wildtype        1950 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   2000

Wildtype        2000 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2050

Wildtype        2050 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2100

Wildtype        2100 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2150

Wildtype        2150 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2151 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2200

Wildtype        2200 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2201 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2250

Wildtype        2250 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2251 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2300

Wildtype        2300 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2301 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2350

Wildtype        2350 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2351 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2400

Wildtype        2400 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2401 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2450

Wildtype        2450 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2451 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2500

Wildtype        2500 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2501 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2550

Wildtype        2550 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2551 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2600

Wildtype        2600 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2601 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2650

Wildtype        2650 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2651 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2700

Wildtype        2700 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2701 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2750

Wildtype        2750 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2751 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2800

Wildtype        2800 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2801 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2850

Wildtype        2850 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2851 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2900

Wildtype        2900 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2901 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2950

Wildtype        2950 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   2999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2951 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   3000

Wildtype        3000 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3001 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3050

Wildtype        3050 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3051 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3100

Wildtype        3100 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3101 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3150

Wildtype        3150 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3151 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3200

Wildtype        3200 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3201 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3250

Wildtype        3250 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3251 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3300

Wildtype        3300 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3301 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3350

Wildtype        3350 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3351 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3400

Wildtype        3400 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3401 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3450

Wildtype        3450 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3451 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3500

Wildtype        3500 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3501 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3550

Wildtype        3550 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3551 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3600

Wildtype        3600 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3601 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3650

Wildtype        3650 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3651 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3700

Wildtype        3700 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3701 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3750

Wildtype        3750 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3751 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3800

Wildtype        3800 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3801 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3850

Wildtype        3850 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3851 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3900

Wildtype        3900 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3901 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3950

Wildtype        3950 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   3999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3951 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   4000

Wildtype        4000 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4001 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4050

Wildtype        4050 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4051 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4100

Wildtype        4100 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4101 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4150

Wildtype        4150 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4151 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4200

Wildtype        4200 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4201 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4250

Wildtype        4250 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4251 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4300

Wildtype        4300 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4301 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4350

Wildtype        4350 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4351 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4400

Wildtype        4400 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4401 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4450

Wildtype        4450 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4451 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4500

Wildtype        4500 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4501 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4550

Wildtype        4550 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4551 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4600

Wildtype        4600 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4601 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4650

Wildtype        4650 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4651 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4700

Wildtype        4700 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4701 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4750

Wildtype        4750 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4751 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4800

Wildtype        4800 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4801 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4850

Wildtype        4850 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4851 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4900

Wildtype        4900 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4901 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4950

Wildtype        4950 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   4999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4951 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   5000

Wildtype        5000 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5001 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5050

Wildtype        5050 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5051 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5100

Wildtype        5100 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5101 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5150

Wildtype        5150 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5151 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5200

Wildtype        5200 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5201 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5250

Wildtype        5250 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5251 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5300

Wildtype        5300 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5301 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5350

Wildtype        5350 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5351 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5400

Wildtype        5400 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5401 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5450

Wildtype        5450 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5451 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5500

Wildtype        5500 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5501 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5550

Wildtype        5550 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5551 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5600

Wildtype        5600 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5601 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5650

Wildtype        5650 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5651 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5700

Wildtype        5700 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5701 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5750

Wildtype        5750 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5751 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5800

Wildtype        5800 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5801 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5850

Wildtype        5850 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5851 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5900

Wildtype        5900 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5901 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5950

Wildtype        5950 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   5999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5951 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   6000

Wildtype        6000 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6001 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6050

Wildtype        6050 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6051 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6100

Wildtype        6100 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6101 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6150

Wildtype        6150 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6151 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6200

Wildtype        6200 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6201 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6250

Wildtype        6250 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6251 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6300

Wildtype        6300 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6301 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6350

Wildtype        6350 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6351 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6400

Wildtype        6400 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6401 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6450

Wildtype        6450 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6451 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6500

Wildtype        6500 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6501 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6550

Wildtype        6550 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6551 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6600

Wildtype        6600 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6601 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6650

Wildtype        6650 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6651 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6700

Wildtype        6700 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6701 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6750

Wildtype        6750 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6751 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6800

Wildtype        6800 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6801 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6850

Wildtype        6850 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6851 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6900

Wildtype        6900 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6901 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6950

Wildtype        6950 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   6999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6951 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   7000

Wildtype        7000 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7001 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7050

Wildtype        7050 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7051 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7100

Wildtype        7100 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7101 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7150

Wildtype        7150 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7151 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7200

Wildtype        7200 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7201 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7250

Wildtype        7250 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7251 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7300

Wildtype        7300 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7301 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7350

Wildtype        7350 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7351 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7400

Wildtype        7400 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7401 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7450

Wildtype        7450 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7451 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7500

Wildtype        7500 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7501 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7550

Wildtype        7550 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7551 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7600

Wildtype        7600 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7601 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7650

Wildtype        7650 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7651 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7700

Wildtype        7700 TGGATGCTTTAA   7711
                     ||||||||||||
Mutation        7701 TGGATGCTTTAA   7712

Is tDNA present:

Mutation sequence:

ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAGGCAAGTCACTTTTGCTAAGAGAAGAAATGG
ACTTCTCAAGAAAGCTTATGAACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAATCGTGGTAAAC
TCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAATTTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCC
TACTTTAACCAAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACTTCTGTGTTCTCTCTCTCTCT
CACTTCAATTATAATGCAAGATCTGAATTGTCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT
CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTGTAAGATCTACTTATTTATATTCTATTTAAA
CTTTTGCGCATATAAAAAAAATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTGAATGTTTTCT
TAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTTAACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAG
ATCATCTGTGGAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGTCATATCTTTTCTTGATTTTA
ATTTTGGTTAATAAAGAAAATGTAACTTTATAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT
AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAACTTGCAATTTTACACTTTATAATTTTTTTT
ACTGTTAAGATTTGATTTGCAGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCTTTGCTTCCCA
AGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAACCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATT
CTTACTAGTGATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATTTGTGACCCGGAGTCTGTATA
TATTGGTAATTCAACAAAAATAACAAATTCAGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT
AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATTTTCGGCTCAAGTGAATTATGTAAAATATAG
TTTATATATTTTTACCCAATCTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTCCCAAGAGTGT
ATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAAATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATT
TTTAATAATTAAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAAAGAGCTTAGAATAATTTTCA
TTATATCCAGAGAGAACTTTGAAGTTTTTCTTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA
CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGTTGTAGAGTCACATGAAATAATTACTACAAC
TTTCATCTTATTGTTGCAGCATGGTGAAAACAATTGAAAAGTATCAACGTTCTGGAAGCCAACCAATCAGCTACTGATAC
TCAGGTACTCTAACATTAAATTTGTTTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCTTTTAT
TTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACATTTTGAATATCCTACAAATCTTTAGTTTGAGACCA
TAAGATTTCAAATACCTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTATTAAAACGGAGGGAG
AATATATTATGTATTCGTGTACTCTTTGATCTATTCATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAA
ACTTTTGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTTAAAATTAGGGTTGTGTTTGAACATG
AACTTAAACTGGAGTTGTTTTAAACTTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCAAGTTT
ATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAATATCCTGGCCAAACGGATTAGTCTCTATATCTAGT
AGCGGACCTGCATTGAGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTATTTATATAGAAGTTT
AAGGAAATCGTATAATTTTTAACATAGAAAAAGTGGATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAA
ATCCTAGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCTTTCGTGTCATTAACTTAATTAATCA
TATTTATTTTCTTCATAACTAATTAGTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTATGTTGA
AATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTTTTCTTATGTTTAGCAAAAGCTATTTTAATATCTTT
GAAGCATATTCACTCAAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTTATCTGATGTCCAGTT
GGAACCCTGACTAAATTTATTTACCAATTCATTCTGACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAAC
GAAATGATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATCCTTAACAAATATTTTCTTCATACCA
AATGATTGAATTTCAGGCCTTCAAATATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCAGTAAT
TATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTTATTGTCATATCACCTATAAAGTCATCACCTAGTCA
GATGATATATTTGGTGAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAAAACTTTTCATTTTCA
ACTCATCATTTGATTTAGCAGGTGGAGCAGATCATTCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGA
AGTTTTCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTTGGAAAATGGCCATAAATGAGTTATT
TTTTATGTAAATAATCAGCATTTTAGTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACTAATGT
TACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAAAGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGG
TATGTTTTGCTCACTATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTATTTTCGAAAATCATTC
TATATATTCTATTTTTTGATGTATAATTAACTTGTTTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTA
TTGATACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCATTAAACACAAAGGACCTTGAGCAGC
TTGAGAATCAATTAGAGTCGTCCTTGAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGATAAAAC
ACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTATATGAATGATTTTAATTCCATTTTAAATGCTATATA
ATTGTTTTATCTTGATTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGTATTGCTCGCGTAGTA
TACACGTTCCATGTCCAATTTTAAGAATATGGATTCAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATAT
TAAATTAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTGTGTTAAAAAGGTGCCATCTAAACTT
TAAGGCAAGTTATACGTAGTGATGGTTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCACATTCA
AAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTGAGGATACTAGGTGGAATAAAATGGAAAATAAGAAT
ATCCAAGAAAAGGTGAGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATTGGGCATGTGATGAGA
AGATGCATAGATGACCTAGTGCAAAGGTGTAAGAAGTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAA
GTATAGCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTACATGACCTCATATAAGAAAGTGTGGA
GGATGAACTAGGACAGAAGAATAGTAAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCCCTCAC
TAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTTTGTTGATATCTATTATTCATGTAGTTTGATTCTAG
CTAGTATATATTTAGTTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAGGTCATCCAAAAAAAA
TTATGAGAACTCTTCTTCAAATATAAAATTTGAAATCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAG
ATGAATAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAAGGTTTTTTTTAAAAAAAATTTAAGA
TATTTTCTTTGGTTTTTGTTACTCTTATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATCTTTTT
GATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATGAATTTTTAAATTTGCAGATTAATTGACATTAAATT
TATAAAAGAAAAAAGTAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAAATCTTTCATAACAAA
TGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTAAGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCA
AAGAAGCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGAGCGTCAGTGATACTGCGATTAGAAG
GTAAACAAAAGTTGTACTAGAAATATTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGATTTTT
TTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTATGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGA
AACAACAAAATGCAGTAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGGCAATGGGGGATGCAT
TGGATATAGGCAAATCGAAAACGTATTAAACAAAAATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATT
AATTTATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATACTAGCTAAAATTAAAGATCAAAATAT
GCTTAGCAAAAACTTATGGAAAATAACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCCTAAGT
GAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTAAAGGGTCGAATAATATATAGTATGGTTACTTATTT
TGCCAACCCTACCAACAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGATCAGCTTGCACAACTT
CAACAAAGGGTACTCATTAGAATTTAAATTTTTTCAAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATT
TCAAATTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAGATTACTCCGCAGAAAGGTAAAACAT
TAATGACCAACCAATTCAAAGATTATATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTATAACT
AGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGTAACTTGATAATGTACAGAGTTTTAATACTGAATTG
AAATTGGTGTTGTGTGTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGCTGGGAAGATAATGGA
GCTGATCAAGCCATGAATCAACACAATCGTCTCCCTCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCC
CCCACAATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTTTATTTCTATTAATAGATGATCAATA
TGCACCAATAAGTTGTTAAATAGATAGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATATGCAAT
GTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTGACCCAGATTGAACTCTTAATAAAAAGGCCAATGTC
CAACATTAAACAATTCTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTTTTGCATAACAAAAAT
AGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAATTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAA
TATATAGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAATATTTGTTGTAAATGTAGTTCCTAGC
AAATAATGGAGGATTGCTGATAGAAACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTATGTTAA
AAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCTATGTTTATATAAGAAAAATATATATAAAAATTGGT
ATAAGATGGAAAAAAAGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAAACCTCACAGCTTTGT
GTGTCATATGTTCAACCTTATTAGCACCCTAACCCCTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTC
GAGCGCTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTACATTATGCAATTCAGATTTAGTCGA
GAGCGATATAGATGGACTATACATGTCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTGCTCCT
TGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAGGGAGGACCTGAATTCACATAAATTTGGATTAAAAA
TTAGGTTTTCTTATACAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTGATTGTTGTTCTGATG
AATTATTTCTAGGTACAATCCTGTTAATACAGATGAGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTC
ATGGGTGGATGCTTTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGAAGGGGAAGAGTAGAACTGAAGAGAATAGAGAACAAAATAAACAG     50

Wildtype          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GCAAGTCACTTTTGCTAAGAGAAGAAATGGACTTCTCAAGAAAGCTTATG    100

Wildtype         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AACTTTCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTCTCTAAT    150

Wildtype         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CGTGGTAAACTCTATGAATTCTGCAGCACTTCAAGGTACTTTTATTTAAT    200

Wildtype         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TTACAACTAAACATTTTAATGTTTTCATTTTCTACCCCCCTACTTTAACC    250

Wildtype         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 AAACCCTTATCTTCTAACTACTTTCATTTTTATTCTTACTTTCATTAACT    300

Wildtype         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TCTGTGTTCTCTCTCTCTCTCACTTCAATTATAATGCAAGATCTGAATTG    350

Wildtype         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TCGTAATATGAGTGAATTGTGTACGTATTTTTACTATATACATATAAGAT    400

Wildtype         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CAATTTTCTCTGTCTGTCTCTCTATATATGTGTTCTAGATTTGATTTTTG    450

Wildtype         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TAAGATCTACTTATTTATATTCTATTTAAACTTTTGCGCATATAAAAAAA    500

Wildtype         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATTGATTTTTTTTCTTTTTAGTTTTTTTCCTCAAGAACAGACTGCTCTTG    550

Wildtype         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 AATGTTTTCTTAAAATTAGTTTTCACGGTGTATATATAGAATTGTTTTTT    600

Wildtype         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 AACCTGCGGATGATCTTTTTTAATGAGTTCTATTGGTGAGATCATCTGTG    650

Wildtype         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GAATGAAACTATTGAGGGTTATTTCTTGAACGATTTTTATTTCGGAAAGT    700

Wildtype         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 CATATCTTTTCTTGATTTTAATTTTGGTTAATAAAGAAAATGTAACTTTA    750

Wildtype         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TAATAACTAATAATTAGTTGAGTGAGAGAAAAAAAAAACTCAAATAATTT    800

Wildtype         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 AGCTAAAACGCTTTGCAGAGGAATTTACTGCCAAGATTCTGAAAACCTAA    850

Wildtype         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 CTTGCAATTTTACACTTTATAATTTTTTTTACTGTTAAGATTTGATTTGC    900

Wildtype         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 AGACTAGGTGCCCATGTTTGACAGTGGCAAAATACTGCTACTTTTGTTCT    950

Wildtype         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TTGCTTCCCAAGGCGGGTTAGCAAAGGCTGCTTATTTTAGTTTCCAATAA   1000

Wildtype        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 CCAGCCAAAGCTGTAATTCTTTTTGTAAATATAATTAATTCTTACTAGTG   1050

Wildtype        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 ATAACTTATTTAATTTAAAGAAATATTTACACCAGATCATTGTCAAAATT   1100

Wildtype        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1101 TGTGACCCGGAGTCTGTATATATTGGTAATTCAACAAAAATAACAAATTC   1150

Wildtype        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1151 AGTTTAATCTTATAAGTGGTGGTCCGGAGACAATGGAGACAATGTTATAT   1200

Wildtype        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1201 AGTCAAGCTTGCTCCTACTTCGTGATGGTAAAGAATGTATATGACTTATT   1250

Wildtype        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1251 TTCGGCTCAAGTGAATTATGTAAAATATAGTTTATATATTTTTACCCAAT   1300

Wildtype        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1301 CTCATGCACCTTCATTATCTAGCCCCAAAATCAATTGTTCCAATTATCTC   1350

Wildtype        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1351 CCAAGAGTGTATATATCTTTATTTGACCCATCTTATTTTACAAACAAGAA   1400

Wildtype        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1401 ATGAAACGAATTTCTGCACGAGTCACAAAATTTTTTTATTTTTAATAATT   1450

Wildtype        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1451 AAATGTGGGATGTTTGTTTAGCACTGACTTCTGTTTGTTAATTGATAGAA   1500

Wildtype        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1501 AGAGCTTAGAATAATTTTCATTATATCCAGAGAGAACTTTGAAGTTTTTC   1550

Wildtype        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1551 TTTATGATCAAGTGGATCGTACCTTCATAATAATGACAACTTCTGCTTCA   1600

Wildtype        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1601 CCCACCTTGCACTGGAAAATGTTCCAAAAAAAGAAAATCTATTAATTTGT   1650

Wildtype        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1651 TGTAGAGTCACATGAAATAATTACTACAACTTTCATCTTATTGTTGCAGC   1700

Wildtype        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCT-ACTCTGG   1749
                     |||||||||||||||||||||||||||||||||||||||||| |||||||
Mutation        1701 ATGGTGAAAACAATTGAAAAGTATCAACGTTGCAGCTATGCTAACTCTGG   1750
gRNA                                           ACGTTGCAGCTATGCT-ACTC

Wildtype        1750 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1751 AAGCCAACCAATCAGCTACTGATACTCAGGTACTCTAACATTAAATTTGT   1800

Wildtype        1800 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1801 TTTTTCATATATATTTTAAAAGAAATGCTTTTTCTTTTTGGGTCAATTCT   1850

Wildtype        1850 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1851 TTTATTTTAGTTTTTTATGTATCATTTTAAAGCTACAAGATTAAAGGACA   1900

Wildtype        1900 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1901 TTTTGAATATCCTACAAATCTTTAGTTTGAGACCATAAGATTTCAAATAC   1950

Wildtype        1950 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   1999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1951 CTTGTTACTCTCTTATACTTCGTACTAGTCAATACTAAACAATCATATTA   2000

Wildtype        2000 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2001 TTAAAACGGAGGGAGAATATATTATGTATTCGTGTACTCTTTGATCTATT   2050

Wildtype        2050 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2051 CATCTAATCTCTAATAATATTATTTGGTCAGTTAGAGACATAAAAACTTT   2100

Wildtype        2100 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2101 TGAAATTTGGGAAAAAATAGTACTTCAATGTAAGTCGAAAATAGTTTTTT   2150

Wildtype        2150 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2151 AAAATTAGGGTTGTGTTTGAACATGAACTTAAACTGGAGTTGTTTTAAAC   2200

Wildtype        2200 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2201 TTTGTGAGTGATTTTAAGGTCAAGTTATATTCGAGAAAATTAATTTGGCA   2250

Wildtype        2250 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2251 AGTTTATGTCAAAACAATTTTCCAATTTAATATTTCAAAAAAAGTAAGAA   2300

Wildtype        2300 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2301 TATCCTGGCCAAACGGATTAGTCTCTATATCTAGTAGCGGACCTGCATTG   2350

Wildtype        2350 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2351 AGTCGGTCGGGAGGTCGAGCACTCATTGACTTTGTGTCGAATTCTATGTA   2400

Wildtype        2400 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2401 TTTATATAGAAGTTTAAGGAAATCGTATAATTTTTAACATAGAAAAAGTG   2450

Wildtype        2450 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2451 GATTACTACCTCATGAGCTAATTAGGGAGAACTCACGACCTTAAAATCCT   2500

Wildtype        2500 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2501 AGATCCGCTACTGTCTGTAGTAATATAAGATTATATATTTAACAATTTCT   2550

Wildtype        2550 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2551 TTCGTGTCATTAACTTAATTAATCATATTTATTTTCTTCATAACTAATTA   2600

Wildtype        2600 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2601 GTTACAGGAATTAAAAGTTATTTCTAAAGTCTGTTTTACATTTTCTTTAT   2650

Wildtype        2650 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2651 GTTGAAATGGTTGAAACATTGTTAATTCTGCATTTCCGTTAATTAACTTT   2700

Wildtype        2700 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2701 TTCTTATGTTTAGCAAAAGCTATTTTAATATCTTTGAAGCATATTCACTC   2750

Wildtype        2750 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2751 AAATTTACTTTCATCCAGCTTTTAGGACTTTTTTTTTCTTTTGGTTGTTT   2800

Wildtype        2800 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2801 ATCTGATGTCCAGTTGGAACCCTGACTAAATTTATTTACCAATTCATTCT   2850

Wildtype        2850 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2851 GACCTGAAGATCATCAAAATTTAATCTAAACATATAAGAGAAAACGAAAT   2900

Wildtype        2900 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2901 GATTTTGAAATGTGGCGCACCGCGTAAGGTCCTTTTAAATGGAATCGATC   2950

Wildtype        2950 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   2999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        2951 CTTAACAAATATTTTCTTCATACCAAATGATTGAATTTCAGGCCTTCAAA   3000

Wildtype        3000 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3001 TATGAGTTTAAGCTTAATTAAAACGAGGGGATCCTATGAATGTGAGACCA   3050

Wildtype        3050 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3051 GTAATTATGGTGAGAGTTGTATTCATCACACCAGCACCCTCTCGTTGGTT   3100

Wildtype        3100 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3101 ATTGTCATATCACCTATAAAGTCATCACCTAGTCAGATGATATATTTGGT   3150

Wildtype        3150 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3151 GAATAATTCTGATTAGAAATGTATTTAAGCAAAACTTTTTAACCTAAAAA   3200

Wildtype        3200 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3201 AACTTTTCATTTTCAACTCATCATTTGATTTAGCAGGTGGAGCAGATCAT   3250

Wildtype        3250 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3251 TCTAATGCTTATAATGAATTGCTTTTAAATTTTTGGCTTGAAAGAAGTTT   3300

Wildtype        3300 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3301 TCGTTTCTGCAATAATTGGAAATGTCACCTTAATGATTGAATGATTTGTT   3350

Wildtype        3350 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3351 GGAAAATGGCCATAAATGAGTTATTTTTTATGTAAATAATCAGCATTTTA   3400

Wildtype        3400 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3401 GTTTTCCAGTCAGTGTTAATGTAGTAATCTTTTCTCAACATTTAATTACT   3450

Wildtype        3450 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3451 AATGTTACATGTTTTCTGACAGAACAACTACCACGAGTATCTGAGGCTAA   3500

Wildtype        3500 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3501 AGGCTAGAGTTGATCTCCTCCAACGAACTCAGAGGTATGTTTTGCTCACT   3550

Wildtype        3550 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3551 ATCCATATATACTTAATTCCTCCGTCTCAAATTAGTTGTCATGTTGTTAT   3600

Wildtype        3600 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3601 TTTCGAAAATCATTCTATATATTCTATTTTTTGATGTATAATTAACTTGT   3650

Wildtype        3650 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3651 TTTCGTTATCTTACATGCTCCCTGCGTATATCCCATTGATTGGTATTGAT   3700

Wildtype        3700 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3701 ACGTTTTGTGAAAATTACACAGAAATTTTCTTGGGGAAGATTTAGGGTCA   3750

Wildtype        3750 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3751 TTAAACACAAAGGACCTTGAGCAGCTTGAGAATCAATTAGAGTCGTCCTT   3800

Wildtype        3800 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3801 GAAGCAAATCAGGTCAAGGAAGGTAAATTATTTAATTTAATACCCTAGAT   3850

Wildtype        3850 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3851 AAAACACAGCGTTATAAGTTTGATGATGGATATGGTGCTTTTGATCTTAT   3900

Wildtype        3900 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3901 ATGAATGATTTTAATTCCATTTTAAATGCTATATAATTGTTTTATCTTGA   3950

Wildtype        3950 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   3999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        3951 TTGCTAGCCCCATACGTTGTCTCATAGATTCTTCTGGGGGATAAGAGCGT   4000

Wildtype        4000 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4001 ATTGCTCGCGTAGTATACACGTTCCATGTCCAATTTTAAGAATATGGATT   4050

Wildtype        4050 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4051 CAATTATATATTATCTAAGAGAGCAAAAAAGATATATATATATATTAAAT   4100

Wildtype        4100 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4101 TAGAGGATGTCACTCACCGTATTGGTGTAGGAAGGATAAAAAGTCATGTG   4150

Wildtype        4150 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4151 TGTTAAAAAGGTGCCATCTAAACTTTAAGGCAAGTTATACGTAGTGATGG   4200

Wildtype        4200 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4201 TTTGACCAGTAATGTTGTATGAGACATGTAGGCTAGTTTAAAACTCTCAC   4250

Wildtype        4250 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4251 ATTCAAAAGATTGAAGGTGAAAAAGTTGAGATTGTCGCGATGGATGTGTG   4300

Wildtype        4300 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4301 AGGATACTAGGTGGAATAAAATGGAAAATAAGAATATCCAAGAAAAGGTG   4350

Wildtype        4350 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4351 AGAGCGACTTAATGAGAGACGAGATGTTAGAAGTAAGGTTGAGATTGATT   4400

Wildtype        4400 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4401 GGGCATGTGATGAGAAGATGCATAGATGACCTAGTGCAAAGGTGTAAGAA   4450

Wildtype        4450 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4451 GTTGGCTATGGATGAATCCAAGAGACGCAGAGATAGACCAAAGAAGTATA   4500

Wildtype        4500 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4501 GCGAGATATGCTTCGATAGGGACATAGCATAGTTAGAGCTTATCGAGTAC   4550

Wildtype        4550 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4551 ATGACCTCATATAAGAAAGTGTGGAGGATGAACTAGGACAGAAGAATAGT   4600

Wildtype        4600 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4601 AAATAGGAGTTCATCCATGCTAGTTGGTAGGAGTGCTTTATCTTATTGCC   4650

Wildtype        4650 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4651 CTCACTAATAGTCATAGTGCTATACTTGTAGTTTCTTGTTTTTAGATTTT   4700

Wildtype        4700 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4701 TGTTGATATCTATTATTCATGTAGTTTGATTCTAGCTAGTATATATTTAG   4750

Wildtype        4750 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4751 TTTGATGTCATATACGGTATATCCAATTCCCCTTGCTTTCACATTATTAG   4800

Wildtype        4800 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4801 GTCATCCAAAAAAAATTATGAGAACTCTTCTTCAAATATAAAATTTGAAA   4850

Wildtype        4850 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4851 TCTTAAATATATGTTAACTGCTACAATTGAGTCTTGTATTTGAAGATGAA   4900

Wildtype        4900 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4901 TAATTACATAGGTCTAATTATGTGACTAATAGTGTAAAGATTGATACAAA   4950

Wildtype        4950 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   4999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        4951 GGTTTTTTTTAAAAAAAATTTAAGATATTTTCTTTGGTTTTTGTTACTCT   5000

Wildtype        5000 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5001 TATGCACACATGTATGTATTGGTAATATGGAAATTTATTTTAGTACAATC   5050

Wildtype        5050 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5051 TTTTTGATTTTTGAAAAGAAACAGGTTGAGAGGTTCAATTAATCAAAATG   5100

Wildtype        5100 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5101 AATTTTTAAATTTGCAGATTAATTGACATTAAATTTATAAAAGAAAAAAG   5150

Wildtype        5150 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5151 TAAAAACCTCTCTCATCCCATATTTTGTTATGTACAAACAAATATCCAAA   5200

Wildtype        5200 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5201 ATCTTTCATAACAAATGTGTGTTAGATAAGAGGTGGTCTATCACAGGGTA   5250

Wildtype        5250 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5251 AGAATTCTCCACTTATAACCTTAAAGTTGTGAATTCAAGTCACCAAAGAA   5300

Wildtype        5300 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5301 GCAAAAAAATGTGAAGCTCCTAGGGGAGGGATTTTCAAAAAAAAATGTGA   5350

Wildtype        5350 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5351 GCGTCAGTGATACTGCGATTAGAAGGTAAACAAAAGTTGTACTAGAAATA   5400

Wildtype        5400 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5401 TTTGTACAACCAATTGAGTGCTGTATTATTTTTTTTAATTTATGCTAAGA   5450

Wildtype        5450 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5451 TTTTTTTGGATGGATTAATGCAGTAAATATATATACAGATTTGGTGCTTA   5500

Wildtype        5500 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5501 TGTTTTTATTCTCTAAGTGTAAATGTTTTTACTGAAACAACAAAATGCAG   5550

Wildtype        5550 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5551 TAGTTTTTTGGAAGTGTAGGAGTTTAATTTTCTCCAAACAGTAGGATTGG   5600

Wildtype        5600 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5601 CAATGGGGGATGCATTGGATATAGGCAAATCGAAAACGTATTAAACAAAA   5650

Wildtype        5650 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5651 ATAGAATATCTGATTCACCATATTTCACCAGGTAAAAGATGGATTAATTT   5700

Wildtype        5700 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5701 ATAGATAATATGGATATTCATGATTTTAGGAATAGTCGGAAAGAAAATAC   5750

Wildtype        5750 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5751 TAGCTAAAATTAAAGATCAAAATATGCTTAGCAAAAACTTATGGAAAATA   5800

Wildtype        5800 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5801 ACAAGCAAACAGATTGATATTTGACTTTAATCATCTGTATATTCTTTTCC   5850

Wildtype        5850 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5851 TAAGTGAATAGTAATTGATCCATTTTTAAAAGGTCAATTATCCAACATTA   5900

Wildtype        5900 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5901 AAGGGTCGAATAATATATAGTATGGTTACTTATTTTGCCAACCCTACCAA   5950

Wildtype        5950 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   5999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        5951 CAACTATGTTTCTTTTGCCCTTTAATTTGCAGACACAATCCATGCTGGAT   6000

Wildtype        6000 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6001 CAGCTTGCACAACTTCAACAAAGGGTACTCATTAGAATTTAAATTTTTTC   6050

Wildtype        6050 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6051 AAATTTTAAATATATGAAAGGATAATTTCAAGAAATTCATTTATTTCAAA   6100

Wildtype        6100 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6101 TTCTTTATGAATTTCAATTTGCAGGAGCAAATGCTGGCAGAATCTAACAG   6150

Wildtype        6150 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6151 ATTACTCCGCAGAAAGGTAAAACATTAATGACCAACCAATTCAAAGATTA   6200

Wildtype        6200 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6201 TATACTGAATTTTTTACACCATAACATATACTACTCATTTTTACCAGTTA   6250

Wildtype        6250 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6251 TAACTAGTAACATGTCTTAGTTTCAAGATTACGGATCTCATAATTTAAGT   6300

Wildtype        6300 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6301 AACTTGATAATGTACAGAGTTTTAATACTGAATTGAAATTGGTGTTGTGT   6350

Wildtype        6350 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6351 GTGTTTTTTAGCTAGAAGAAAGTGCGGCTGGATTTCCAGTTCGACTGTGC   6400

Wildtype        6400 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6401 TGGGAAGATAATGGAGCTGATCAAGCCATGAATCAACACAATCGTCTCCC   6450

Wildtype        6450 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6451 TCACACAGAGGGTTTCTTTCAGCCTCTTGGATTGCATTCTTCCCCCCCAC   6500

Wildtype        6500 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6501 AATTTGGGTAATTATTACTATTATTAAATATAGTTTTTCGTTTTATCTTT   6550

Wildtype        6550 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6551 TATTTCTATTAATAGATGATCAATATGCACCAATAAGTTGTTAAATAGAT   6600

Wildtype        6600 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6601 AGTAATAGAAGGAAAGACTCTAAAAACCTTAAGAGTTACTTGGAAAATAT   6650

Wildtype        6650 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6651 GCAATGTGAGTGAGTGAGATGAACAGTGGTCACATAATTAACACAAGTTG   6700

Wildtype        6700 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6701 ACCCAGATTGAACTCTTAATAAAAAGGCCAATGTCCAACATTAAACAATT   6750

Wildtype        6750 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6751 CTTCTTTTTTTTCTTTTATTTTCTTTAAATGGTGAAACTTACTTATCGTT   6800

Wildtype        6800 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6801 TTGCATAACAAAAATAGTAAGTTTCACCATAAAAAAAATGGGAAAAAGAA   6850

Wildtype        6850 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6851 TTGGGACACAGGACTTACTTATTATATAGTATGCATCATAAGAAATATAT   6900

Wildtype        6900 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6901 AGTCACATGTATGATTTTAGAGTCAAGTGGATCATAATATAAATCGAAAT   6950

Wildtype        6950 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   6999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        6951 ATTTGTTGTAAATGTAGTTCCTAGCAAATAATGGAGGATTGCTGATAGAA   7000

Wildtype        7000 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7001 ACATTGGTTGTGGTGCAGTGGTAGGCTGTTGCACCTAAGAGCGGAATTAT   7050

Wildtype        7050 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7099
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7051 GTTAAAAAATATGGTATTTCGGCACCCATTAAACTTGACGAAAAAGAGCT   7100

Wildtype        7100 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7101 ATGTTTATATAAGAAAAATATATATAAAAATTGGTATAAGATGGAAAAAA   7150

Wildtype        7150 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7151 AGCACCCATCAAACCAAAAAGATAGCTGAGTGCTTTGATTTTCAGGCGAA   7200

Wildtype        7200 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7201 ACCTCACAGCTTTGTGTGTCATATGTTCAACCTTATTAGCACCCTAACCC   7250

Wildtype        7250 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7251 CTGGGTCCGCTACTTGTTTCATCCTTAACCAGAGGTCTTGAGTTCGAGCG   7300

Wildtype        7300 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7301 CTAAAAATGAAAAAACTTATATTAGGAGCGTCACCCCTTAATGGGCTCTA   7350

Wildtype        7350 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7351 CATTATGCAATTCAGATTTAGTCGAGAGCGATATAGATGGACTATACATG   7400

Wildtype        7400 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7401 TCCCATAAAACTTTCTTCTTCCTTGCTAATGTGTCATCGACCTTATTCTG   7450

Wildtype        7450 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7451 CTCCTTGTACGTATGCCTTAGCTGTAGGATGCCAATTTGGCTCATCGCAG   7500

Wildtype        7500 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7501 GGAGGACCTGAATTCACATAAATTTGGATTAAAAATTAGGTTTTCTTATA   7550

Wildtype        7550 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7551 CAATTAATTCTAGACAGCTCATTTAATTATCATTATTATTATTTCATTTG   7600

Wildtype        7600 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7601 ATTGTTGTTCTGATGAATTATTTCTAGGTACAATCCTGTTAATACAGATG   7650

Wildtype        7650 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        7651 AGGTGAATGCATCAGCAACTGCGCACAATATGAATGGATTTATTCATGGG   7700

Wildtype        7700 TGGATGCTTTAA   7711
                     ||||||||||||
Mutation        7701 TGGATGCTTTAA   7712