Plant Crispr Database

Plant Information

Name: CRT0006-15-10

Seed stock: 5085
Seed availability: No

Relationship:
Parent: CRT0006-15

Transformation: CRT0006
transformation CRT0006 to RG-prf3

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0006-15-10 (T1 )	CRT0006	CTG0005-2	CGR0005-2	gene : Solyc05g053230 (Pti1b)	azygous Is tDNA present: No Mutation sequence: GGATAACTATGAGCTGCTTCGGTTGTTGTGACGATGATGACATGCATAAACCTGCTGATCATGGACCATTCATGACCAAC AATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGACTCTTTGAAATAATAAATTCAGCTG CAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAGCTCAACCTTAACCACTGAATACTGA TGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCCTATCAGGTCTATAAGTAAATTAGTG CAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACCTGGTATGGGAGATATGCCTGCTCTG CAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAGTCAGGTGAAAGTTGTAGTGTTTAGT ACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCATGATTTTACTCGCTGAGATTCTATT AATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTTTAGGCTACAATGCAGGGCAGCGTGT AACAGAAAGTGCGCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGTTCCTTCTATAACTGTTGATGAGTTAA AAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATGGAAGGGTATACCATGGTGTCCTGAAA AGTGGGCGGGCTGCAGCCATTAAAAAGTTAGACTCGAGCAAGCAACCTGATCGAGAATTTTCAGCACAGGTCAGGTCGTT TGGATTGTCCTACTTTGAACTATTCTATATTATACATAAACCTCTTACCTTGCTTGGATATGATGTTACCATTTCAGGTT TCCATGGTATCAAGACTAAAACATGAAAACGTGGTTGAGTTACTCGGTTATAGTGTGGATGGCGGTCTTCGTGTGCTGGC TTATGAGTATGCCCCCAATGGATCTCTCCATGACATTCTTCATGGTTAGTTTCTATAAGCCTTTGTGTATCCATTCTATG CATGTTGACCAATTAATGATTTGGTTGTTCTAATCTATATATACCGGTGAAATTCAGATTTGGTTGTTCTAATTTATATC CATCTTGGTACGTTGAGTTTGGAAATGATTGCGCAATCCAAATCCAACTCGGTCATGCGCCTTCCCTATAGTGTGCACTT TAAGTACTTGACTACTGCACCCTCAAGAAGTTGTCAAAGCTCCTCCCCCAATATATCCCCTTTCTTGAAGTACCTCACCA GGGTCTTTTTTGACATGTGTCATGGAAGTGTCATGTAAAGTTGCTCTAATCAAGAGTAGACTTGCACCAACACTTCCTCA GATTTTATGCTACTAATTGGACAAACCCTTAAGTATCTCCTTCTTAATTTCAGATTTTATCCTTTTCTTCTCTGTTGTGC ATATACATTTCTTTCTCTTGTCCTCTTCTCAAATATATATTTCATTCATAAGTTCCATTTGTTACGTACTTGACTGTGCA TATAGTGAAAAAGTTTTCTACATATTATATTGGTGGTGACTGGACAGACTTCTGAAAATGATAAGTTGTTAAAATGGAAA ATGTCTACATATAAGTTTTAAGTTCCTCAGATTGAGTAGCTATTAATCCTAATGACAATAGGGGTTTGAACTCATGCTTG CTTTAACCTAACCTACTTGGATCCAGATTTTGGTATATTGGGTTTCAATGTATAATTCTGTCCAATCTGGTTCAAGACTG TGTTAAACATTTTGGACGTGTAAAATTGGACAGGAAGTGTGCACTGGGTTAAGATATAGGGTTTACGGGATATCTGAAGG TAGAATATTATTATTTTTAGTGTGCTTCCATTCCCCTAGTAGCCTTTTGATAGTCCAAACTTGGAAAGACTGCTGTCACT AAGGTCATTGCGATTGGCCCACCTCATTTATCAGATATCGGAAGGATAATCTATCAAGGAAGTAGGGAGAGGAAGAGAGC TTGAAGTGGGTTTCTACATCATACTGATGAAGCTTTATACGGAGATGGTTGTTCCTATATATTTTGACCCAATGTAATTG AATTTGATTGCAGTCGCGTAAGATGCTCTTCTTTTTCTTCAAAATTTAGTCTAACCCACCTTTTTCTCATGTTAATTTCA GCGATTGGCACTGCTCCGTTAGTAGGTAAAAGAAAAGGATAAAATCCTAGTATCTTGGAAGTCCATCATTATCCTGCCAA ATCAATTGCATCCTTCCTCTCTCTCTCTCCCTTATATGTAAATTCTAATATATGGGAACAGCTCCAAAAAGTGATGTTTT GGGGTTGTATTTACCTATCCATTTCCACTATGTTAAGCAAAATAAAACTTCTGCCGTGCAATTGCTGTTTAATGAAAATT GTTGTCATTTGCTGACAGCATGTTGTATTTCTATTTAACAGAGCAAATTCCGTAACAAGCCTTTGTTTTCTCTTACAATT TTCCTTATTTGAACTATTTTCTGACTACGAGGGTATCTATCTCTGTACATATGCGTGTGTATACATATTCCTGTTTTATT TGGAAGTTTTTGTTGTGTTTTCCTTCTTTATGTATGGCTGATCATTCTCATTTTTTTTACTTTTCTGGAACAACTATTGA TTGTGATGGTTGTCTCAGCCAAGTTAATGTGTCATCTCATACTAATGCTGAAAACTGATCCGACTACCTATTTTTTCAAA TTGGTTGTAGCAGCCTCAAGCTACCTGGATTATAAATTATTTTTTTGATGACAAAAGAAATATAAAAAAATATATTCCAC GTAGCCTGGGGCTGCTACAATCAATTTGTAATTCTTGAAATAGTTAGTCAAATTGGGTTTCTCATATTCCATAATCTTGC TAATCCAATCTGTAAAATGCAAATAACTTTTCAATTTGTAATTACAGGACGAAAAGGTGTGAAAGGTGCACAGCCAGGTC CAGTATTATCATGGGCCCAACGGGTTAAAATTGCCGTTGGAGCTGCAAAAGGGCTAGAGTACTTGCATGAAAAAGCACAA CCACATATTATCCATCGTGATATTAAGTCTAGCAATGTCCTACTCTTTGATGATGATGTTGCTAAGATTGCGGATTTTGA TTTATCAAATCAAGCTCCTGATATGGCAGCACGCCTTCACTCCACACGTGTTCTTGGAACCTTTGGTTATCATGCTCCTG AGTGAGTTCACTTCTTAATATTTGATACAAATAGACTGTAAACTTGGTTAACTCACTTAAATGAAGCAATTTTAGCTCAG GGCCCAAAGGTCCTTCATTTTTATGCTGAATGGACTTTGCTCATGTTGCAGATATGCAATGACTGGTCAGCTGAGTTCAA AGAGTGATGTTTACAGCTTTGGTGTTGTCCTCCTTGAACTACTCACTGGTCGTAAACCTGTTGATCATACATTACCACGT GGCCAACAGAGTCTTGTGACATGGGTATAACCCTCAAGATACTGACATTACTATAACTGATGCACCAATTTTTTAGTTGA TTATTTTGATTCTGAATTGACTTTCTGCACCATGTGTAATCAGGCAACACCGAGACTTAGTGAAGATAAAGTGAAGCAAT GTGTTGACGCTAGACTCGGTACAGATTACCCTCCTAAGGCCATTGCAAAGGTATGTCACTAGAGCAGAAAATTCTTGACT ACTAAAATCATAATCCTGCATCTTCTTACATCTCTTCTTGATTTTTCATCAATTCCTATTTCTCAAATTTTGTGTTGCTG AAACAGATGGCTGCTGTTGCTGCTTTGTGTGTGCAATACGAAGCTGATTTTCGGCCAAATATGAGCATTGTGGTAAAAGC TCTACAACCTCTTTTGCACGCTCGACCTGCACCTAGTGAAACATCAAACTTGTGATTCTCTCCTTTGCCGCAGCCGCTAC CAAATGGAATGACAGTGATTGAGATTTGTTTTGTTGAGTTGAAAATGATATTATACTAGTAGTTATCTGGGTAAATAGCA GACATAATGTGCTTTCCCCCCTTCTTTTACTGGTCTCAATTGAATGTGAATAAATTTATACATGTACTAATGAAAGTAGC CTTTGCCTATTCTTTTAATGGATATTATTCTTGAGGTCTTGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50 Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100 Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150 gRNA TTG-CGCACTTTCTGTTAC Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200 gRNA TTGCGCACTTTCTGTTACAC Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250 Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300 Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350 Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400 Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450 Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500 Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550 Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600 Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650 Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700 Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
CRT0006-15-10 (T1 )	CRT0006	CTG0005-1	CGR0005-1	gene : Solyc12g098980 (Pti1a)	-5 bp/-5 bp Is tDNA present: No Mutation sequence: GAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACACAATTCAGCAGGTTAG TTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGAT ATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATT TGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGG GAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGT AATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAA CATTTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2527 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 2576 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 50 Wildtype 2577 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 2626 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 100 Wildtype 2627 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 2676 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 150 Wildtype 2677 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 2726 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 200 Wildtype 2727 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 2776 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 250 Wildtype 2777 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 2826 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 300 Wildtype 2827 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 2876 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 350 Wildtype 2877 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 2926 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 400 Wildtype 2927 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 2976 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 450 Wildtype 2977 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 3026 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 500 Wildtype 3027 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 3076 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 550 Wildtype 3077 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 3126 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 551 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 600 Wildtype 3127 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 3176 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 601 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 650 Wildtype 3177 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 3226 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 651 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 700 Wildtype 3227 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 3276 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 701 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 750 Wildtype 3277 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 3326 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 751 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 800 Wildtype 3327 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 3376 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 801 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 850 Wildtype 3377 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 3426 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 851 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 900 Wildtype 3427 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 3476 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 901 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 950 gRNA GCTGAATTGTGTGCCATGAA Wildtype 3477 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 3525 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 951 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 999

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0006-15-10 (T1 )

CRT0006

CTG0005-2

CGR0005-2

gene : Solyc05g053230 (Pti1b)

azygous

Is tDNA present: No

Mutation sequence:

GGATAACTATGAGCTGCTTCGGTTGTTGTGACGATGATGACATGCATAAACCTGCTGATCATGGACCATTCATGACCAAC
AATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGACTCTTTGAAATAATAAATTCAGCTG
CAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAGCTCAACCTTAACCACTGAATACTGA
TGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCCTATCAGGTCTATAAGTAAATTAGTG
CAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACCTGGTATGGGAGATATGCCTGCTCTG
CAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAGTCAGGTGAAAGTTGTAGTGTTTAGT
ACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCATGATTTTACTCGCTGAGATTCTATT
AATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTTTAGGCTACAATGCAGGGCAGCGTGT
AACAGAAAGTGCGCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGTTCCTTCTATAACTGTTGATGAGTTAA
AAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATGGAAGGGTATACCATGGTGTCCTGAAA
AGTGGGCGGGCTGCAGCCATTAAAAAGTTAGACTCGAGCAAGCAACCTGATCGAGAATTTTCAGCACAGGTCAGGTCGTT
TGGATTGTCCTACTTTGAACTATTCTATATTATACATAAACCTCTTACCTTGCTTGGATATGATGTTACCATTTCAGGTT
TCCATGGTATCAAGACTAAAACATGAAAACGTGGTTGAGTTACTCGGTTATAGTGTGGATGGCGGTCTTCGTGTGCTGGC
TTATGAGTATGCCCCCAATGGATCTCTCCATGACATTCTTCATGGTTAGTTTCTATAAGCCTTTGTGTATCCATTCTATG
CATGTTGACCAATTAATGATTTGGTTGTTCTAATCTATATATACCGGTGAAATTCAGATTTGGTTGTTCTAATTTATATC
CATCTTGGTACGTTGAGTTTGGAAATGATTGCGCAATCCAAATCCAACTCGGTCATGCGCCTTCCCTATAGTGTGCACTT
TAAGTACTTGACTACTGCACCCTCAAGAAGTTGTCAAAGCTCCTCCCCCAATATATCCCCTTTCTTGAAGTACCTCACCA
GGGTCTTTTTTGACATGTGTCATGGAAGTGTCATGTAAAGTTGCTCTAATCAAGAGTAGACTTGCACCAACACTTCCTCA
GATTTTATGCTACTAATTGGACAAACCCTTAAGTATCTCCTTCTTAATTTCAGATTTTATCCTTTTCTTCTCTGTTGTGC
ATATACATTTCTTTCTCTTGTCCTCTTCTCAAATATATATTTCATTCATAAGTTCCATTTGTTACGTACTTGACTGTGCA
TATAGTGAAAAAGTTTTCTACATATTATATTGGTGGTGACTGGACAGACTTCTGAAAATGATAAGTTGTTAAAATGGAAA
ATGTCTACATATAAGTTTTAAGTTCCTCAGATTGAGTAGCTATTAATCCTAATGACAATAGGGGTTTGAACTCATGCTTG
CTTTAACCTAACCTACTTGGATCCAGATTTTGGTATATTGGGTTTCAATGTATAATTCTGTCCAATCTGGTTCAAGACTG
TGTTAAACATTTTGGACGTGTAAAATTGGACAGGAAGTGTGCACTGGGTTAAGATATAGGGTTTACGGGATATCTGAAGG
TAGAATATTATTATTTTTAGTGTGCTTCCATTCCCCTAGTAGCCTTTTGATAGTCCAAACTTGGAAAGACTGCTGTCACT
AAGGTCATTGCGATTGGCCCACCTCATTTATCAGATATCGGAAGGATAATCTATCAAGGAAGTAGGGAGAGGAAGAGAGC
TTGAAGTGGGTTTCTACATCATACTGATGAAGCTTTATACGGAGATGGTTGTTCCTATATATTTTGACCCAATGTAATTG
AATTTGATTGCAGTCGCGTAAGATGCTCTTCTTTTTCTTCAAAATTTAGTCTAACCCACCTTTTTCTCATGTTAATTTCA
GCGATTGGCACTGCTCCGTTAGTAGGTAAAAGAAAAGGATAAAATCCTAGTATCTTGGAAGTCCATCATTATCCTGCCAA
ATCAATTGCATCCTTCCTCTCTCTCTCTCCCTTATATGTAAATTCTAATATATGGGAACAGCTCCAAAAAGTGATGTTTT
GGGGTTGTATTTACCTATCCATTTCCACTATGTTAAGCAAAATAAAACTTCTGCCGTGCAATTGCTGTTTAATGAAAATT
GTTGTCATTTGCTGACAGCATGTTGTATTTCTATTTAACAGAGCAAATTCCGTAACAAGCCTTTGTTTTCTCTTACAATT
TTCCTTATTTGAACTATTTTCTGACTACGAGGGTATCTATCTCTGTACATATGCGTGTGTATACATATTCCTGTTTTATT
TGGAAGTTTTTGTTGTGTTTTCCTTCTTTATGTATGGCTGATCATTCTCATTTTTTTTACTTTTCTGGAACAACTATTGA
TTGTGATGGTTGTCTCAGCCAAGTTAATGTGTCATCTCATACTAATGCTGAAAACTGATCCGACTACCTATTTTTTCAAA
TTGGTTGTAGCAGCCTCAAGCTACCTGGATTATAAATTATTTTTTTGATGACAAAAGAAATATAAAAAAATATATTCCAC
GTAGCCTGGGGCTGCTACAATCAATTTGTAATTCTTGAAATAGTTAGTCAAATTGGGTTTCTCATATTCCATAATCTTGC
TAATCCAATCTGTAAAATGCAAATAACTTTTCAATTTGTAATTACAGGACGAAAAGGTGTGAAAGGTGCACAGCCAGGTC
CAGTATTATCATGGGCCCAACGGGTTAAAATTGCCGTTGGAGCTGCAAAAGGGCTAGAGTACTTGCATGAAAAAGCACAA
CCACATATTATCCATCGTGATATTAAGTCTAGCAATGTCCTACTCTTTGATGATGATGTTGCTAAGATTGCGGATTTTGA
TTTATCAAATCAAGCTCCTGATATGGCAGCACGCCTTCACTCCACACGTGTTCTTGGAACCTTTGGTTATCATGCTCCTG
AGTGAGTTCACTTCTTAATATTTGATACAAATAGACTGTAAACTTGGTTAACTCACTTAAATGAAGCAATTTTAGCTCAG
GGCCCAAAGGTCCTTCATTTTTATGCTGAATGGACTTTGCTCATGTTGCAGATATGCAATGACTGGTCAGCTGAGTTCAA
AGAGTGATGTTTACAGCTTTGGTGTTGTCCTCCTTGAACTACTCACTGGTCGTAAACCTGTTGATCATACATTACCACGT
GGCCAACAGAGTCTTGTGACATGGGTATAACCCTCAAGATACTGACATTACTATAACTGATGCACCAATTTTTTAGTTGA
TTATTTTGATTCTGAATTGACTTTCTGCACCATGTGTAATCAGGCAACACCGAGACTTAGTGAAGATAAAGTGAAGCAAT
GTGTTGACGCTAGACTCGGTACAGATTACCCTCCTAAGGCCATTGCAAAGGTATGTCACTAGAGCAGAAAATTCTTGACT
ACTAAAATCATAATCCTGCATCTTCTTACATCTCTTCTTGATTTTTCATCAATTCCTATTTCTCAAATTTTGTGTTGCTG
AAACAGATGGCTGCTGTTGCTGCTTTGTGTGTGCAATACGAAGCTGATTTTCGGCCAAATATGAGCATTGTGGTAAAAGC
TCTACAACCTCTTTTGCACGCTCGACCTGCACCTAGTGAAACATCAAACTTGTGATTCTCTCCTTTGCCGCAGCCGCTAC
CAAATGGAATGACAGTGATTGAGATTTGTTTTGTTGAGTTGAAAATGATATTATACTAGTAGTTATCTGGGTAAATAGCA
GACATAATGTGCTTTCCCCCCTTCTTTTACTGGTCTCAATTGAATGTGAATAAATTTATACATGTACTAATGAAAGTAGC
CTTTGCCTATTCTTTTAATGGATATTATTCTTGAGGTCTTGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA   3465
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA     50

Wildtype        3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA   3515
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA    100

Wildtype        3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC   3564
                     |||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev      101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC    150
gRNA                                                TTG-CGCACTTTCTGTTAC

Wildtype        3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC   3614
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC    200
gRNA                 TTGCGCACTTTCTGTTACAC

Wildtype        3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA   3664
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA    250

Wildtype        3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA   3714
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA    300

Wildtype        3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC   3764
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC    350

Wildtype        3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT   3814
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT    400

Wildtype        3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT   3864
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT    450

Wildtype        3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT   3914
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT    500

Wildtype        3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC   3964
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC    550

Wildtype        3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA   4014
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA    600

Wildtype        4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA   4064
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA    650

Wildtype        4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT   4114
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT    700

Wildtype        4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA   4147
                     |||||||||||||||||||||||||||||||||
MutationRev      701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA    733

CRT0006-15-10 (T1 )

CRT0006

CTG0005-1

CGR0005-1

gene : Solyc12g098980 (Pti1a)

-5 bp/-5 bp

Is tDNA present: No

Mutation sequence:

GAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACACAATTCAGCAGGTTAG
TTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGAT
ATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATT
TGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGG
GAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGT
AATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAA
CATTTA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2527 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA   2576
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA     50

Wildtype        2577 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA   2626
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA    100

Wildtype        2627 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA   2676
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA    150

Wildtype        2677 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT   2726
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT    200

Wildtype        2727 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA   2776
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA    250

Wildtype        2777 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT   2826
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT    300

Wildtype        2827 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG   2876
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG    350

Wildtype        2877 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG   2926
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG    400

Wildtype        2927 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA   2976
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA    450

Wildtype        2977 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA   3026
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA    500

Wildtype        3027 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC   3076
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC    550

Wildtype        3077 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT   3126
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      551 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT    600

Wildtype        3127 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT   3176
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      601 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT    650

Wildtype        3177 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT   3226
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      651 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT    700

Wildtype        3227 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT   3276
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      701 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT    750

Wildtype        3277 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC   3326
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      751 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC    800

Wildtype        3327 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG   3376
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      801 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG    850

Wildtype        3377 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT   3426
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      851 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT    900

Wildtype        3427 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT   3476
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      901 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT    950
gRNA                                    GCTGAATTGTGTGCCATGAA

Wildtype        3477 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT   3525
                     |||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      951 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT    999