Plant Crispr Database

Plant Information

Name: CRT0138-7-7

Seed stock: 4971
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0138-7

Transformation: CRT0138
transformation CRT0138 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0138-7-7 (T1 )	CRT0138	CTG0138-2	CGR0138-2	gene : Solyc02g094060 (Transporter gene)	-2 bp/-2 bp Is tDNA present: No Mutation sequence: ACTCATACTGAACCTTATCTGTTTTGGCAGAGATAATGAAATAGATTTGTTGTATTTACATGTCATTATGTGTCTCTGAT TTAGGATCAATGAAAATGATCTCTTCAAGCATGATTGGAGAAAAAGATCAAGAGTTCAAACATTACAGTATGTCTTCATC AAATGGACACTCGCATTCCTGGTCGGCCTTTTTACCGGAGGTCGCCACCCTCATCAGTCTCTCAATCGAAAATATCTCTG GTTACAAACTCCGCGCTACTGTCAACTATATTGATGACAAAAGGTTAATAAGTAGTAAAATTTATTTCTCGTCTCGTTTG TTCTAATGACTTACAATTATGATAGTCTGACTTAATTAAAACAATTATGTAGGTACTTTATGGGATTTGCATTTTTTGCG GGTGCTAACTTTCTGTTGACATTGCTAGCTGCCTTTATCACTGTTTATTTTGCACCTACGGCTGCCGGGCCTGGAGTTCC AGAAATTAAAGCCTATCTCAATGGGGTAGACACTCCCAATATGTTCGGGGCAACTACACTCTTTGTTAAGGTTAATGCTG ATTTTTATCAACAGTGTTCTCATTTACCCTCTAGCATGACTTGAGACCACAACTTATCCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2368 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT 2417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT 50 Wildtype 2418 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT 2467 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT 100 Wildtype 2468 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG 2517 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG 150 Wildtype 2518 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT 2567 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT 200 Wildtype 2568 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT 2617 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT 250 Wildtype 2618 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA 2667 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA 300 Wildtype 2668 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA 2717 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA 350 Wildtype 2718 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG 2767 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG 400 Wildtype 2768 AGACTGATGAGGGTGGCGACACCTCCGGTAAAAAGGCCGACCAGGAATGC 2817 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 AGACTGATGAGGGTGGCG--ACCTCCGGTAAAAAGGCCGACCAGGAATGC 448 gRNA GATGAGGGTGGCGACACCTC Wildtype 2818 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT 2867 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 449 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT 498 Wildtype 2868 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA 2917 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 499 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA 548 Wildtype 2918 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA 2967 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 549 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA 598 Wildtype 2968 ACAGATAAGGTTCAGTATGAGT 2989 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 599 ACAGATAAGGTTCAGTATGAGT 620	Morphology : Retarded growth; Germination: 90%
CRT0138-7-7 (T1 )	CRT0138	CTG0138-12	CGR0138-12	gene : Solyc05g005910 (Peptide Transporter 1)	azygous Is tDNA present: No Mutation sequence: GAAATACCAGCAGCATCATTCAGTGTATTCATGATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGT CCCTTTGCTATCAAAATATACTGGACAGCCAAGAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCT GTATGTCTATGGCGTTGTCAGCAATAACAGAAAGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCA AGTGCGTTAGTGAACATGTCTGCTATGTGGCTCGTACCACAGTATGCACTACTCGGAGTGGCTGAGGGTTCCCATTCTGT TGGACAGATTGAGTTCTTCTACTCTCTGTTACCCAAAAGCATGTCCAGCATGGCATCAGCTATGTACACTGTTGGGACTG CTGTGTCGAGTTTGGTTGTAAGTATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAACTGGAGGCAAAACTAGCTGGCTT TCGAGCAACATTAATAGGGGTCACCTAGATTACTATTTCTGGCTACTTACTTTTTTGAGTTTGCTCAACTTCTTCTATTT TC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 175 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA 224 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA 50 Wildtype 225 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC 274 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC 100 Wildtype 275 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA 324 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA 150 Wildtype 325 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC 374 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC 200 Wildtype 375 AGTCCCAACAGTGTACATAGCTGATGCCATGCTGGACATGCTTTTGGGTA 424 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AGTCCCAACAGTGTACATAGCTGATGCCATGCTG---------------- 234 Wildtype 425 ACAGAGAGTAGAAGAACTCAATCTGTCCAACAGAATGGGAACCCTCAGCC 474 MutationRev 234 -------------------------------------------------- 234 gRNA TCAGCC Wildtype 475 ACTCCGAGTAGTGCATACTGTGGTACGAGCCACATAGCAGACATGTTCAC 524 \|\|\|\|\|\|\|\|\|\|\| MutationRev 235 ---------------------------------------GACATGTTCAC 245 gRNA TCAGCCACTCCGAGTAGTGC Wildtype 525 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC 574 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 246 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC 295 Wildtype 575 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT 624 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 296 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT 345 Wildtype 625 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT 674 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 346 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT 395 Wildtype 675 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA 724 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 396 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA 445 Wildtype 725 TTGTTAAAGCAATAATC 741 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 446 TTGTTAAAGCAATAATC 462	Morphology : Retarded growth; Germination: 90%

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0138-7-7 (T1 )

CRT0138

CTG0138-2

CGR0138-2

gene : Solyc02g094060 (Transporter gene)

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

ACTCATACTGAACCTTATCTGTTTTGGCAGAGATAATGAAATAGATTTGTTGTATTTACATGTCATTATGTGTCTCTGAT
TTAGGATCAATGAAAATGATCTCTTCAAGCATGATTGGAGAAAAAGATCAAGAGTTCAAACATTACAGTATGTCTTCATC
AAATGGACACTCGCATTCCTGGTCGGCCTTTTTACCGGAGGTCGCCACCCTCATCAGTCTCTCAATCGAAAATATCTCTG
GTTACAAACTCCGCGCTACTGTCAACTATATTGATGACAAAAGGTTAATAAGTAGTAAAATTTATTTCTCGTCTCGTTTG
TTCTAATGACTTACAATTATGATAGTCTGACTTAATTAAAACAATTATGTAGGTACTTTATGGGATTTGCATTTTTTGCG
GGTGCTAACTTTCTGTTGACATTGCTAGCTGCCTTTATCACTGTTTATTTTGCACCTACGGCTGCCGGGCCTGGAGTTCC
AGAAATTAAAGCCTATCTCAATGGGGTAGACACTCCCAATATGTTCGGGGCAACTACACTCTTTGTTAAGGTTAATGCTG
ATTTTTATCAACAGTGTTCTCATTTACCCTCTAGCATGACTTGAGACCACAACTTATCCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2368 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT   2417
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT     50

Wildtype        2418 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT   2467
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT    100

Wildtype        2468 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG   2517
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG    150

Wildtype        2518 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT   2567
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT    200

Wildtype        2568 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT   2617
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT    250

Wildtype        2618 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA   2667
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA    300

Wildtype        2668 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA   2717
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA    350

Wildtype        2718 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG   2767
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG    400

Wildtype        2768 AGACTGATGAGGGTGGCGACACCTCCGGTAAAAAGGCCGACCAGGAATGC   2817
                     ||||||||||||||||||  ||||||||||||||||||||||||||||||
MutationRev      401 AGACTGATGAGGGTGGCG--ACCTCCGGTAAAAAGGCCGACCAGGAATGC    448
gRNA                      GATGAGGGTGGCGACACCTC

Wildtype        2818 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT   2867
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      449 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT    498

Wildtype        2868 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA   2917
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      499 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA    548

Wildtype        2918 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA   2967
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      549 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA    598

Wildtype        2968 ACAGATAAGGTTCAGTATGAGT   2989
                     ||||||||||||||||||||||
MutationRev      599 ACAGATAAGGTTCAGTATGAGT    620

Morphology : Retarded growth; Germination: 90%

CRT0138-7-7 (T1 )

CRT0138

CTG0138-12

CGR0138-12

gene : Solyc05g005910 (Peptide Transporter 1)

azygous

Is tDNA present: No

Mutation sequence:

GAAATACCAGCAGCATCATTCAGTGTATTCATGATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGT
CCCTTTGCTATCAAAATATACTGGACAGCCAAGAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCT
GTATGTCTATGGCGTTGTCAGCAATAACAGAAAGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCA
AGTGCGTTAGTGAACATGTCTGCTATGTGGCTCGTACCACAGTATGCACTACTCGGAGTGGCTGAGGGTTCCCATTCTGT
TGGACAGATTGAGTTCTTCTACTCTCTGTTACCCAAAAGCATGTCCAGCATGGCATCAGCTATGTACACTGTTGGGACTG
CTGTGTCGAGTTTGGTTGTAAGTATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAACTGGAGGCAAAACTAGCTGGCTT
TCGAGCAACATTAATAGGGGTCACCTAGATTACTATTTCTGGCTACTTACTTTTTTGAGTTTGCTCAACTTCTTCTATTT
TC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         175 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA    224
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 CCATCATTGTCAGGTTCATATAACCGGCAGATGAGCAGAAAATAGAAGAA     50

Wildtype         225 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC    274
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GTTGAGCAAACTCAAAAAAGTAAGTAGCCAGAAATAGTAATCTAGGTGAC    100

Wildtype         275 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA    324
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 CCCTATTAATGTTGCTCGAAAGCCAGCTAGTTTTGCCTCCAGTTGATGAA    150

Wildtype         325 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC    374
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 AGCCAATCCACACCACTCACCAGAATACTTACAACCAAACTCGACACAGC    200

Wildtype         375 AGTCCCAACAGTGTACATAGCTGATGCCATGCTGGACATGCTTTTGGGTA    424
                     ||||||||||||||||||||||||||||||||||                
MutationRev      201 AGTCCCAACAGTGTACATAGCTGATGCCATGCTG----------------    234

Wildtype         425 ACAGAGAGTAGAAGAACTCAATCTGTCCAACAGAATGGGAACCCTCAGCC    474
                                                                       
MutationRev      234 --------------------------------------------------    234
gRNA                                                             TCAGCC

Wildtype         475 ACTCCGAGTAGTGCATACTGTGGTACGAGCCACATAGCAGACATGTTCAC    524
                                                            |||||||||||
MutationRev      235 ---------------------------------------GACATGTTCAC    245
gRNA                 TCAGCCACTCCGAGTAGTGC

Wildtype         525 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC    574
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      246 TAACGCACTTGGGTCGTCCTCATGTCCTTCCTCTATTGCCAATTGTCGTC    295

Wildtype         575 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT    624
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      296 TTATGCTTTCTGTTATTGCTGACAACGCCATAGACATACAGGTGACTATT    345

Wildtype         625 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT    674
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      346 AAGCCAATACCCATTCGGATGACAGGACTTAGCCCTCTTGGCTGTCCAGT    395

Wildtype         675 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA    724
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      396 ATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAGGTAATCCAGA    445

Wildtype         725 TTGTTAAAGCAATAATC    741
                     |||||||||||||||||
MutationRev      446 TTGTTAAAGCAATAATC    462

Morphology : Retarded growth; Germination: 90%